Method for breeding pittosporum tobira seedlings based on tissue culture technology

Abstract

The invention discloses a method for breeding pittosporum tobira seedlings based on a tissue culture technology. The method comprises the following steps of: selecting excellent single plants, selecting and preparing explants, performing induction culture, performing multiplication culture, performing rooting culture, hardening seedlings and transplanting. According to the method disclosed by theinvention, the pittosporum tobira seedlings are cultured by reasonably selecting the explants, controlling an induction culture medium and induction culture conditions, controlling a multiplication culture medium and multiplication culture conditions and controlling a rooting culture medium and rooting culture conditions. According to the method, a large number of excellent pittosporum tobira seedlings consistent with female parent characters can be provided in a short time, so that the problem that the reproductive rate of cutting propagation and seed propagation is low in the prior art is solved.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for breeding Pittosporum seedlings based on tissue culture technology. Background technique [0002] Scaevola sericea, also known as cleats horn tree, water plant, and fine-leaf water plant, belongs to the perennial evergreen subshrub of the family Scaevola sericea. Pittosporum likes high temperature, humid and sunny environment, good salt tolerance, strong wind resistance, drought resistance, cold resistance, strong resistance to pollution and pest damage, and fast growth speed, so it can be used as windproof and sand-fixing vegetation in islands and coastal zones alternative tree species. In addition, after research and development, it was found that Pittosporum also contains a variety of secondary metabolites, which have important medicinal value and can treat cataracts, stomach problems, scaly skin ringworm, knife wounds and animal bites, and have...

AI Technical Summary

Problems solved by technology

At present, the main propagation methods of Pittodia are seed propagation and cutting propagation, but because the seeds are not easy to collect and the germination rate is low, the reproduction rate of Pitotonia is low, and at the same time, it is difficult for the offspring to maintain the excellent traits of the original variety due to the instability of seed inheritance. , while the cutting propagation is limited by the source of the parent plant of Pittonia pratense, the propagation efficiency is low, and it is difficult to carry out large-scale production

With the development of biotechnology, a tissue culture technology specially used for the rapid propagation of plant seedlings began to appear. The tissue culture technology has the advantages of fast reproduction, short cycle, and can provide a large number of excellent seedlings with the same traits as the female parent in a short period of time. For the characteristics of production, it is expected to become a new method to provide the provenance of Pittosporum, but there is no mature report on the tissue culture of Pittosporum at present

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