Injectable double-drug-loading compound chitosan hydrogel and preparation method thereof

A technology of compounding chitosan water and dual drugs is applied in the field of medical biomaterials, which can solve the problem that the drug-loaded hydrogel cannot be applied in stages, and achieves the effects of good application prospects, resistance to inflammation, and no special requirements.

Active Publication Date: 2020-06-23
XIAN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] An object of the present invention is to provide an injectable double drug-loaded composite chitosan hydr

Method used

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  • Injectable double-drug-loading compound chitosan hydrogel and preparation method thereof
  • Injectable double-drug-loading compound chitosan hydrogel and preparation method thereof

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preparation example Construction

[0032] A kind of preparation method of injectable double-drug loaded composite chitosan hydrogel of the present invention comprises the following steps:

[0033] Step 1, preparing liposomes, fully dissolving lipids and osteoporosis drugs with a mass ratio of 0.5-1.5:0.01-0.5 in ether, then evaporating to remove ether in the solution, adding phosphate buffer to the solution, mixing Evenly, the drug-loaded liposome solution is obtained, and the drug-loaded liposome solution is dried and ground to obtain liposomes, and the lipid is phospholipid or sterol;

[0034] Step 2, taking an appropriate amount of chitosan and adding it into an acetic acid solution with a concentration of 0.525g / l to 3.15g / l, stirring evenly to obtain a light yellow transparent chitosan solution, the amount of chitosan in the chitosan solution The concentration is 2g / l~50g / l;

[0035] Step 3, Preparation of Hydrogel

[0036] Step 3.1, add liposomes into the chitosan solution, then add calcium nitrate, dis...

Embodiment 1

[0041] A kind of preparation method of injectable loaded double drug composite chitosan hydrogel is prepared, comprising the following steps:

[0042] Step 1, preparation of osteoporosis drug-loaded liposomes

[0043] Dissolve 1 g of phospholipids and 0.05 g of active vitamin D in ether in a container. After fully dissolving, remove the ether by rotary evaporation to form a liposome film on the surface of the container. Add 5 mL of phosphate buffer at pH=7 to the solution. The liposome membrane is fully shaken to hydrate and fall off to obtain a liposome solution, which is then dried and ground to obtain the osteoporosis drug-loaded liposome.

[0044] Step 2, prepare chitosan solution

[0045] Weigh 0.1g of chitosan and add it into 15mL of acetic acid solution, the concentration of the acetic acid solution is 0.525g / l, and fully dissolve it by magnetic stirring at 40°C to obtain a light yellow transparent liquid, namely chitosan solution;

[0046] Step 3, Preparation of Hydr...

Embodiment 2

[0052] A kind of preparation method of injectable loaded double drug composite chitosan hydrogel is prepared, comprising the following steps:

[0053] Step 1, preparation of osteoporosis drug-loaded liposomes

[0054] Dissolve 1.5g of phospholipids and 0.05g of alendronate sodium in ether in a container. After fully dissolving, remove the ether by rotary evaporation to form a liposome film on the surface of the container. Add 10mL of phosphate at pH=7 to the solution The buffer solution is fully shaken to hydrate and shed the liposome membrane to obtain a liposome solution, which is then dried and ground to obtain the osteoporosis drug-loaded liposome.

[0055] Step 2, prepare chitosan solution

[0056] Weigh 0.3g of chitosan and add it into 35mL of acetic acid solution, the concentration of the acetic acid solution is 1.5g / l, and fully dissolve it by magnetic stirring at 40°C to obtain a light yellow transparent liquid, namely chitosan solution;

[0057] Step 3, Preparation...

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Abstract

The invention discloses an injectable double-drug-loading compound chitosan hydrogel. The compound chitosan hydrogel contains liposomes, a chitosan solution, antibiotics, hydroxyapatite and a cross-linking agent, wherein the liposomes contain lipoids and osteoporosis drugs in a mass ratio of (0.5-1.5) to (0.01-0.5), the chitosan solution consists of chitosan and acetic acid; and the hydroxyapatiteis prepared from inorganic minerals and an alkaline solution. The invention further discloses a preparation method of the injectable double-drug-loading compound chitosan hydrogel. By virtue of different cross-linking degrees of the hydrogel prepared by virtue of the method, the hydrogel can be released in different stages, is capable of resisting inflammations and treating osteoporosis for a long time and has good application prospects in the biomedical field.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and relates to an injectable double-drug loaded composite chitosan hydrogel and a preparation method thereof. Background technique [0002] With the aging of the world's population, a series of bone defects such as osteoporosis, rheumatoid arthritis, bone cracks, and bone tumors are caused, which greatly increases the clinical demand for orthopedic drugs and orthopedic filling materials. Common causes of bone defects include inflammation, tumors, open fractures, etc. Therefore, it is necessary to combine anti-inflammation, anti-tumor, and fracture healing drugs for treatment while repairing bone defects. [0003] Bone defect is a common clinical orthopedic disease, and at present, autologous or allogeneic bone transplantation is mostly used for treatment. Transplantation therapy can cause damage to the donor site and donors are difficult to obtain, so its application is limited. In...

Claims

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Application Information

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IPC IPC(8): A61K9/06A61K45/06A61K47/36A61L27/46A61L27/52A61L27/54A61P19/10A61P29/00A61K31/59A61K31/593A61K31/65A61K31/66A61K31/7036A61K38/14A61K9/127
CPCA61K9/06A61K9/0024A61K47/36A61K45/06A61L27/52A61L27/54A61L27/46A61P29/00A61P19/10A61K31/7036A61K38/14A61K31/65A61K31/59A61K31/66A61K31/593A61L2400/06A61L2300/406A61L2300/41A61L2300/626A61K9/127C08L5/08A61K2300/00Y02A50/30
Inventor 汤玉斐孙雅妮陈磊赵康吴子祥蒋吓树
Owner XIAN UNIV OF TECH
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