Quintuple fluorescence polymerase chain reaction (PCR) detection system, application thereof and quintuple fluorescence PCR detection product

Abstract

The invention relates to the technical field of biology, and in particular to a quintuple fluorescence polymerase chain reaction (PCR) detection system, an application thereof and a quintuple fluorescence PCR detection product. The detection system is used for performing fluorescence detection by using fluorescent dyes with emission wavelengths of 470 nm-525 nm, 523 nm-564 nm, 571 nm-612 nm, 628 nm-692 nm and 678 nm-718 nm respectively. According to the detection system, fluorescent dyes with emission wavelengths positioned in five specific wave bands are used as detection dyes for quintuple fluorescence PCR, quintuple detection in a same PCR reaction system can be realized through five detection channels (wave bands) capable of being used for simultaneous detection in the same PCR reaction system, and crosstalk phenomenon exists among detection results of the five detection channels. The results are accurate and the detection system is suitable for high-throughput detection.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a five-fold fluorescent PCR detection system and its application and products. Background technique [0002] Fluorescent PCR technology still belongs to PCR technology in essence, but the difference is that fluorescent PCR adds a chemical group that can emit fluorescence at a specific wavelength after being excited by excited light on the basis of the original PCR technology, and realizes it through the accumulation of fluorescent signals. The purpose of real-time monitoring of the whole process of PCR. At present, the signal characterization methods involved in fluorescent PCR technology are mainly divided into dye method and probe method. Dye-based fluorescent PCR mostly uses SYBR Green I as the signal carrier. The characteristic of this dye is that it can bind to the minor groove in the DNA double helix structure. When the dye binds to DNA and is emitted by light of a certain wav...

AI Technical Summary

Problems solved by technology

However, the current fluorescent PCR technology is limited by the lack of available fluorescent channels and the difficult optimization of the same-tube multiplex PCR system, so it is difficult to meet the above two requirements

According to the survey, the same-tube multi-color fluorescent PCR method that can be commercialized on a large scale currently only achieves four colors in the same tube, and some of the same-tube multi-color fluorescent PCR methods have serious crosstalk between the detection channels.

Images