Arsenic trioxide nanoparticles carrying transition metal complex and preparation method of arsenic trioxide nanoparticles

An arsenic trioxide, transition metal technology, applied in the fields of nanotechnology, nanotechnology, nanotechnology for materials and surface science, and can solve the problems of short maintenance time, low bioavailability, and low concentration

Pending Publication Date: 2020-06-26
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, ATO exists in the form of arsenous acid in aqueous solution, resulting in low concentration at the lesion site, short maintenance time, and low bioavailability

Method used

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  • Arsenic trioxide nanoparticles carrying transition metal complex and preparation method of arsenic trioxide nanoparticles
  • Arsenic trioxide nanoparticles carrying transition metal complex and preparation method of arsenic trioxide nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 3

[0025] The preparation of embodiment 1 arsenic trioxide nanoparticles

[0026] Accurately weigh 20mg PEG 5K -PLGA 20k Dissolve 2.5mg CO-520 in 1mL dichloromethane to form an organic phase; accurately weigh 50mg arsenic trioxide and dissolve it in sodium hydroxide, then adjust the pH with hydrochloric acid (pH=8) to prepare arsenic trioxide solution; take 50μL arsenic trioxide solution respectively Add water and 0.5mL organic phase, probe ultrasonic (over 2s, stop 2s) for 1 minute to obtain emulsion, mix the two emulsions and vortex for 5 minutes, then add to 5mL water probe ultrasonic (over 2s, stop 2s) for 2 minutes , followed by rotary evaporation at 40°C for 20 minutes to remove dichloromethane to obtain nanoparticles.

Embodiment 2

[0027] Example 2 Preparation of arsenic trioxide nanoparticles loaded with arsenic-nickel metal complex

[0028] Accurately weigh 20mg PEG 5K -PLGA 20k Dissolve 2.5mg CO-520 in 1mL dichloromethane to form an organic phase; accurately weigh 50mg of arsenic trioxide and dissolve it in sodium hydroxide, then adjust the pH with hydrochloric acid (pH=8) to prepare arsenic trioxide solution, and accurately weigh 60mg of acetic acid Dissolve nickel in 1mL water to obtain nickel acetate solution; add 50μL arsenic trioxide solution and nickel acetate solution to 0.5mL organic phase respectively, probe ultrasonically (super 2s, stop 2s) for 1 minute to obtain emulsion, mix the two emulsions and vortex for 5 Minutes later, it was added to 5mL water and the probe was sonicated (super 2s, stop 2s) for 2 minutes, and then 40°C rotary evaporation for 20 minutes to remove dichloromethane to obtain nanoparticles. The electron microscope photo is shown in figure 1 .

Embodiment 3

[0029] Example 3 Preparation of arsenic trioxide nanoparticles loaded with arsenic-manganese metal complex

[0030] Accurately weigh 20mg PEG 5K -PLGA 20k Dissolve 2.5mg CO-520 in 1mL dichloromethane to form an organic phase; accurately weigh 50mg of arsenic trioxide and dissolve it in sodium hydroxide, then adjust the pH with hydrochloric acid (pH=8) to prepare arsenic trioxide solution, and accurately weigh 60mg of acetic acid Dissolve manganese in 1mL water to obtain manganese acetate solution; add 50μL of arsenic trioxide solution and manganese acetate solution to 0.5mL organic phase respectively, probe ultrasonically (super 2s, stop 2s) for 1 minute to obtain emulsion, mix the two emulsions and vortex for 5 Minutes later, it was added to 5mL of water and the probe was sonicated (super 2s, stop 2s) for 2 minutes, followed by rotary evaporation at 40°C for 20 minutes to remove dichloromethane to obtain nanoparticles.

[0031] Determination of cell viability: K562 cells we...

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Abstract

The invention discloses arsenic trioxide nanoparticles carrying a transition metal complex. The arsenic trioxide nanoparticles include the arsenite complex and a carrier, the arsenite complex is carried on the carrier, and the arsenite complex is composed of arsenic and transition metal ions, wherein the weight ratio of the arsenic to the transition metal ion is 1:(1-3). Based on the fact that arsenic ions can form an arsenite complex with most transition metals, the nanoparticles using high-molecular polymer polyethylene glycol-poly(lactic-co-glycolic acid) as the carrier material are designed and synthesized. The invention aims to provide an efficient preparation method of the arsenic trioxide nanoparticles. When the nanoparticles reach a lesion, an acidic tumor microenvironment can decompose the arsenite complex to release ATO to achieve the effect of treating diseases.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to arsenic trioxide nanoparticles loaded with transition state metal complexes and a preparation method. Background technique [0002] Arsenic trioxide, commonly known as arsenic, has the molecular formula As 2 o 3 , which can be abbreviated as ATO. ATO has been used as a medicine in Western and traditional Chinese medicine for more than two thousand years. ATO has been approved by the US FDA for the treatment of relapsed and refractory acute promyelocytic leukemia (APL), with a remission rate of 70%-90%. However, ATO exists in the form of arsenous acid in aqueous solution, resulting in low concentration at the lesion site, short maintenance time and low bioavailability. Therefore, it is necessary to study [0003] Study a new type of arsenic trioxide preparation. [0004] A variety of studies have shown that nanodrugs can increase the concentra...

Claims

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Application Information

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IPC IPC(8): A61K33/36A61K9/14A61K47/34A61P35/00B82Y5/00B82Y30/00
CPCA61K9/146A61K33/36A61P35/00B82Y5/00B82Y30/00
Inventor 穆朝峰范露慧
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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