Micro-particle suspension volume concentration micro-fluidic device

Abstract

The invention discloses a micro-particle suspension volume concentration micro-fluidic device, which is composed of a sample inlet, series spiral flow channels, a cross-flow filtering flow channel, aY-shaped bifurcated flow channel, a concentrated sample outlet and a confluence flow channel connected with a blank liquid outlet, wherein the sample inlet is connected with the series spiral flow channels, the cross-flow filtering flow channel is arranged between the series spiral flow channels, the tail ends of the series spiral flow channels comprise a sample outlet end and a liquid outlet end,the Y-shaped bifurcated flow channel is connected to the sample outlet ends of the tail ends of the series spiral flow channels, the concentrated sample outlet is connected with the Y-shaped bifurcated flow channel, and the confluence flow channel is connected with the liquid outlet end of the tail ends of the series spiral flow channels. The device can realize high-flux and high-magnification concentration of a micron particle suspension, the optimal recovery rate is as high as 99.99%, precise flow resistance matching is not needed, the operation is simple, the damage to cells is low, the activity of the treated cells is as high as 95%, the structure is simple, and the cost is low.

Description

technical field [0001] The present invention relates to a volume concentration microfluidic device, more particularly, to a micron particle suspension volume concentration microfluidic device. Background technique [0002] Biological suspensions containing cells or particles are important medical samples. The counting and detection of cells or particles in these biological suspensions can provide important information for disease diagnosis. The detection of cells or particles in biological suspensions requires sample Concentration pretreatment improves the sensitivity and detection rate of detection by increasing the concentration of target components in the sample. Currently, the commonly used sample concentration methods include: high-speed centrifugation and membrane filtration. The centrifugation method uses strong centrifugal force to force the cells or particles to settle to the bottom of the centrifuge tube, and then removes the supernatant to reduce the sample volume...

AI Technical Summary

Problems solved by technology

However, the centrifugation method is expensive and cannot be used in complex working conditions such as the field. At the same time, it is not capable of efficiently concentrating samples with extremely low cell / particle concentration or super-large volume samples, and strong centrifugal force will cause damage and death of biological particles such as cells.

Membrane filtration uses filter holes of specific pore size on the filter membrane to block cells or particles, and increase the concentration of the sample by filtering out the blank liquid. This method is simple and direct, but it is difficult to recover cells blocked on the filter membrane. The clogging problem will cause the efficiency to decrease sharply with the use time, and the fixed filter hole is only suitable for the filtration of cells or particles of a specific size, and the scope of application is small

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