Urease-nanogold composite material with adjustable urease activity as well as preparation method and application of composite material

A technology of composite materials and urease activity, applied in biochemical equipment and methods, nanotechnology for materials and surface science, nanotechnology, etc., can solve problems such as complexity, bulkiness, and low detection efficiency, and achieve an improved degree of decomposition Effect

Active Publication Date: 2020-06-26
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, the activity of urease reaches the strongest at a temperature of about 60°C, and the activity is not high at room temperature, which leads to problems such as low detection efficiency.
At present, in order to speed up the detection, it is mainly realized by heating, but the heating method often requires a water bath or an oven, which is bulky and complicated.

Method used

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  • Urease-nanogold composite material with adjustable urease activity as well as preparation method and application of composite material
  • Urease-nanogold composite material with adjustable urease activity as well as preparation method and application of composite material
  • Urease-nanogold composite material with adjustable urease activity as well as preparation method and application of composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Preparation of hollow porous nano gold

[0022] Add 640μL of 28mM hydroquinone to 18mL, 90mM PVP solution, add 40μL, 60mM AgNO 3 After the solution is protected from light, add 640μL of 25mM chloroauric acid drop by drop, shake while adding, shake vigorously for 5min and then let stand for 60min, add 200μL of concentrated ammonia water, react in a shaker at 37℃ for 2h, centrifuge and disperse into 2mL water to obtain 1.5mg / mL of porous hollow nano-gold solution.

[0023] (2) Synthesis of gold nanoparticles modified by urease

[0024] Add 240 μL of the nano-gold solution obtained in step (1) to 360 μL, 1 mg / mL urease, mix and shake overnight, and centrifuge to obtain urease-functionalized nano-gold.

[0025] (3) Light and heat control urease activity

[0026] Use with a power of 1.5W / cm 2 The near-infrared light with a wavelength of 808nm irradiates the urease-modified gold nanospheres and a separate urease solution for 10 minutes, and the results are as follows figure 2 As...

Embodiment 2

[0029] (1) Preparation of hollow porous nano gold

[0030] Add 600μL of 30mM hydroquinone to 17mL, 100mM PVP solution, add 50μL, 50mM AgNO 3 After the solution is protected from light, add 600μL of 30mM chloroauric acid drop by drop, shake while adding, shake vigorously for 6min and let stand for 70min, add 300μL of 12mM concentrated ammonia water, react in a shaker at 35℃ for 3h, centrifuge and disperse into 1.5mL water to obtain 2mg / mL of porous hollow nano-gold solution.

[0031] (2) Synthesis of gold nanoparticles modified by urease

[0032] Add 300 μL of the nano-gold solution obtained in step (1) to 400 μL, 1.5 mg / mL urease, mix and shake overnight, and centrifuge to obtain urease-functionalized gold nanoparticles.

Embodiment 3

[0034] (1) Preparation of hollow porous nano gold

[0035] Add 700μL of 25mM hydroquinone to 19mL, 80mM PVP solution, add 30μL, 70mM AgNO 3 After the solution is protected from light, add 700μL of 20mM chloroauric acid drop by drop, shake while adding, shake vigorously for 4min and let stand for 50min, add 100μL of 14mM concentrated ammonia water, react at 40℃ shaker for 1h, centrifuge and disperse into 3mL water to obtain 1.0mg / mL of porous hollow nano-gold solution.

[0036] (2) Synthesis of gold nanoparticles modified by urease

[0037] Add 200 μL of the nano-gold solution obtained in step (1) to 300 μL, 0.5 mg / mL urease, mix and shake overnight, and centrifuge to obtain urease-functionalized nano-gold.

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Abstract

The invention discloses a urease-nanogold composite material with adjustable urease activity as well as a preparation method and application of the composite material. The composite material is formedby combining urease and nanogold, wherein the mass ratio of the urease to the nanogold is (0.5-1.5):(0.5-1.5). The urease modified nanogold synthesized by method can effectively control the catalyticactivity of the urease through a photothermal effect; a mixture of urea and phenol is used as a substrate, and under laser irradiation conditions, the degree of decomposition of the substrate by theurease-modified nanogold within the same reaction time is greatly improved; and the irradiation power of laser is changed, the degree of decomposition of the substrate by the urease-modified nanogoldchanges, and the catalytic activity of the urease can be adjusted by changing the laser power.

Description

Technical field [0001] The invention belongs to the technical field of nano materials and biological detection, and specifically relates to a urease-nano gold composite material with adjustable urease activity, and a preparation method and application thereof. Background technique [0002] Urease is a nickel-containing oligomerase with absolute specificity and can specifically catalyze the decomposition of urea to release ammonia and carbon dioxide. It is often used to determine urea in urine and blood. Under normal circumstances, the activity of urinary enzyme reaches its strongest at a temperature of about 60°C, and the low activity at room temperature leads to problems such as low detection efficiency. At present, to speed up the detection speed is mainly achieved by heating, but the heating method often requires a water bath or an oven, which is bulky and complicated. In recent years, nanomaterials with photothermal effects have received widespread attention. Because nanopar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C12Q1/58B22F1/00B22F9/24B82Y30/00B82Y40/00
CPCC12N11/14C12N9/80C12Q1/58C12Y305/01005B22F9/24B82Y30/00B82Y40/00B22F1/0553B22F1/07B22F1/054
Inventor 衣馨瑶童柳娟胡盛强王建秀
Owner CENT SOUTH UNIV
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