Soft tissue repairing material as well as preparation method and application thereof

A repair material and soft tissue technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of poor color texture integration, difficult maintenance, and difficult surgery of the recipient site

Active Publication Date: 2020-07-03
SHENZHEN LANDO BIOMATERIALS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Autologous tissue transplantation has always been the gold standard in the industry, but there are still deficiencies, such as pain and complications caused by the new trauma site, limited supply of transplanted tissue, grafted mucosal necrosis, poor color texture integration of the recipient site, and difficult surgery Da et al. In order to effectively avoid these shortcomings, soft tissue repair materials have been gradually developed and utilized.
[0003] When oral soft tissue repair materials are used for oral soft tissue repair, they must have good biocompatibility, be degradable in vivo, allow gingival tissue to grow in, have certain mechanical support properties (can maintain the volume of soft tissue), and prevent wound inflammation or Cracking can promote wound healing. However, the current oral soft tissue repair materials are still difficult to maintain the balance between tissue volume and tissue integrity (inflammation or cracking), that is, they cannot solve the problem of having good mechanical support performance while having good The issue of biocompatibility

Method used

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  • Soft tissue repairing material as well as preparation method and application thereof
  • Soft tissue repairing material as well as preparation method and application thereof
  • Soft tissue repairing material as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0031] like figure 1 Shown, the preparation method of the soft tissue repair material of one embodiment, comprises the following steps:

[0032] Step S110: Decellularize, dehydrate and degrease the animal tissue to obtain a dry product.

[0033] At this time, the main component of the obtained dried product is collagen fibers.

[0034] Specifically, the steps of decellularizing, dehydrating and degreasing the animal tissue include: alternately washing the animal tissue with a strong alkali solution and a strong acid solution for decellularization, and then using an organic solvent to dehydrate and degrease the washed animal tissue respectively deal with.

[0035] Specifically, the mass percent concentration of the strong alkali solution is 1%-5%. More specifically, the strong alkali solution is selected from at least one of sodium hydroxide aqueous solution and potassium hydroxide aqueous solution.

[0036] Specifically, the mass percent concentration of the strong acid so...

Embodiment 1

[0065] The preparation process of the soft tissue repair material of this embodiment is as follows:

[0066] (1) The aqueous solution of potassium hydroxide of 3% and the hydrochloric acid of 0.5% by mass percent concentration are carried out alternate cleaning with the dermal tissue of pig, alternately 2 times; 70%, 80%, 90%, and 100% ethanol for 3 hours each, and then soaked in absolute ethanol for 12 hours to obtain a dry product.

[0067] (2) Grind the dried product obtained in step (1) using a cutting mill, and then sieve it so that the median particle diameter of the dried product is 1 mm to obtain a powdery dried product, and dissolve the powdered dried product at a pH of 3 in the acid solution to obtain a slurry, wherein the dry matter in the slurry has a mass percentage of 7%. Wherein, the acid solution is hydrochloric acid.

[0068] (3) Adding a cross-linking agent to the slurry in step (2) to carry out a cross-linking reaction, and then dialysis to remove the rema...

Embodiment 2

[0073] The preparation process of the soft tissue repair material of this embodiment is as follows:

[0074] (1) The peritoneum of the pig is washed alternately with 1% sodium hydroxide aqueous solution and 1% hydrochloric acid with a mass percentage concentration of 4 times; then soaked in 70% mass percentage concentration respectively , 80%, 90% and 100% acetone solutions for 0.5 hours each, and then soaked in acetone for 24 hours to obtain dry matter.

[0075] (2) Grind the dried product obtained in step (1) using a cutting mill, and then sieve it so that the median particle diameter of the dried product is 2 mm to obtain a powdered dried product, and dissolve the powdered dried product at a pH of 2 to obtain a slurry, wherein the mass percentage of the dry matter in the slurry is 4%. Wherein, the acid solution is acetic acid.

[0076] (3) Adding a cross-linking agent to the slurry in step (2) to carry out a cross-linking reaction, and then dialysis to remove the remainin...

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Abstract

The invention relates to a soft tissue repairing material as well as a preparation method and application thereof. The preparation method of the soft tissue repairing material comprises the followingsteps: carrying out decellularization, dehydration and degreasing on animal tissues, so as to obtain a dried substance; dissolving the dried substance into an acid solution, so as to obtain slurry; adding a cross-linking agent into the slurry to generate cross-linking reaction, so as to obtain reaction liquid; drying and crushing human amniotic membranes, so as to obtain amniotic membrane powder;and dispersing the amniotic membrane powder into the reaction liquid, and carrying out drying, so as to obtain the soft tissue repairing material, wherein the mass ratio of the amniotic membrane powder to the dried substance in the soft tissue repairing material is (1 to 4)-(1 to 1). The soft tissue repairing material prepared by virtue of the preparation method has relatively good mechanical supporting performance and good biocompatibility.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a soft tissue repair material and its preparation method and application. Background technique [0002] Sufficient amounts of attached keratinized tissue help maintain teeth, periodontal ligaments, and implants. The incidence rate of receding gums in healthy people is as high as more than 50%, and the degree of receding increases the weight of with people's age. The clinical method of treating gingival recession—periodontal gingival surgery has gradually developed and matured, and its application scope has also expanded from patients with periodontal disease to patients with dental aesthetics before implant restoration and orthodontic patients. It is currently very important clinically. Technology. Restorative materials used in periodontal gingival surgery can be roughly divided into two categories, namely, autologous soft tissue grafts and soft tissue replacement materials....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/50A61L27/58A61L27/36
CPCA61L27/50A61L27/58A61L27/3633A61L27/3641A61L2430/34A61L2430/40
Inventor 钟梅玲李丽花朱勇军康文亭佘振定谭荣伟
Owner SHENZHEN LANDO BIOMATERIALS
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