Antigen epitope polypeptide located on nostructal protein 1 of dengue virus and application of antigen epitope polypeptide

A non-structural protein, antigenic epitope technology, applied in the direction of viral peptides, viruses, fusion polypeptides, etc., can solve the problems of indistinguishability, difficult immunological diagnosis and differential diagnosis, etc.

Active Publication Date: 2020-07-07
中国人民解放军南部战区疾病预防控制中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the extensive cross-reactivity of antibodies between flaviviruses, it is difficult to distinguish whether it is the result of flavivirus vaccination or the result of viral infection. Antibodies make immunological diagnosis and differential diagnosis very difficult. The key to solving this problem lies in the screening of high-quality antigens

Method used

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  • Antigen epitope polypeptide located on nostructal protein 1 of dengue virus and application of antigen epitope polypeptide
  • Antigen epitope polypeptide located on nostructal protein 1 of dengue virus and application of antigen epitope polypeptide

Examples

Experimental program
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Effect test

Embodiment 3

[0055] Example 3 Establishment of DENV-ELISA-IgM Detection Technology Based on Recombinant NS269 Trimeric Antigen, Method Specificity and Clinical Evaluation

[0056]Recombinant NS269 trimer antigen-coated enzyme-linked plate (diluted to 2 μg / ml in PBS), 100 μl per well, discarded after 14 hours at 4°C overnight, rinsed with distilled water 3 times, patted dry, added 100 μl of 1% BSA to each well, room temperature 2h, discard the liquid. After adding 100 μl sample diluent to each well, add 10 μl serum of the sample to be tested, incubate at 37°C for 30 minutes, discard the solution, wash the plate 5 times with washing solution, discard the solution, pat dry, and add horseradish peroxidase-labeled anti-human IgM (1:1000) 100μl, incubate at 37°C for 20min, discard the solution and wash the plate 5 times and pat dry. Add TMB color development solution: 50 μl each of solution A and B, develop color at 37°C in the dark for 10 minutes, add 50 μl of stop solution to each well, and r...

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Abstract

The invention discloses an antigen epitope polypeptide located on a nostructal protein 1 of a dengue virus and application of the antigen epitope polypeptide. An antigen polypeptide with high reactivity is screened out from a sequence of the nostructal protein 1 of the dengue virus and is subjected to recombinant expression. Verified by tests, the polypeptide can have relatively high reactivity toIgM in blood serum of a dengue virus infected patient. The antigen epitope polypeptide disclosed by the invention has a huge potential value in early diagnosis on DENV infections and epidemiologicalinvestigations.

Description

technical field [0001] The invention relates to an antigenic epitope polypeptide of dengue virus, in particular to an antigenic epitope polypeptide located in nonstructural protein 1 of dengue virus and application thereof. Background technique [0002] Dengue fever caused by dengue virus (DENV) is a major public health problem in tropical and subtropical countries. Dengue fever epidemics occur almost every year in southern China, especially in the southeastern coastal provinces. DENV belongs to the Flavivirus genus of the family Flaviviridae, members of the Flaviviridae family also include yellow fever virus (yellow fever virus, YFV), West Nile virus (West Nile virus, WNV), Japanese encephalitis virus (Japanese encephalitis virus, JEV) and tick-borne encephalitis virus (tick-borne encephalitis virus, TBEV) and so on. DENV has serotypes I to IV (DENV-1, -2, -3 and -4). Either type of DENV infection can be asymptomatic or cause mild self-limited disease (dengue fever, dengu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/18C07K19/00G01N33/569
CPCC07K14/005G01N33/56983C12N2770/24122C07K2319/00Y02A50/30
Inventor 陈月任瑞文刘乐斌陈荣华刘军张锦海余楠刘朵朵
Owner 中国人民解放军南部战区疾病预防控制中心
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