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Surface-enhanced Raman rapid detection method for rhodamine B in chili powder chili oil

A surface-enhanced Raman and detection method technology, applied in Raman scattering, measuring devices, instruments, etc., can solve the problems of high cost, cumbersome processing, and high requirements for operators

Inactive Publication Date: 2020-07-07
OPTOTRACE SUZHOU TECH
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Problems solved by technology

[0003] The detection methods of rhodamine B in condiments used in existing reports include ultra-high performance liquid chromatography-tandem mass spectrometry, solid phase extraction-ultra high performance liquid chromatography-electrospray tandem mass spectrometry, liquid chromatography-tandem mass spectrometry, and liquid chromatography-tandem mass spectrometry. Mass spectrometry internal standard method, although the detection limit is low, the sensitivity and accuracy are high, but the pretreatment process is cumbersome, the cycle is long, the cost is high, the requirements for operators are high, and the practicability is greatly limited

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  • Surface-enhanced Raman rapid detection method for rhodamine B in chili powder chili oil

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Embodiment Construction

[0032] The present invention will be described in further detail below in conjunction with the examples, but the present invention is not limited to the following examples.

[0033] A surface-enhanced Raman rapid detection method for rhodamine B in chili powder, specifically comprising the following steps:

[0034] (1) Using rhodamine B standard substance and blank paprika, prepare 0.02 mg / kg rhodamine B positive paprika sample and blank negative paprika sample;

[0035] (2) Add 1 g sample and 3 ml n-hexane-acetone mixed solution (volume ratio 1:9) into a 10 ml centrifuge tube, shake for 20 s, and let stand to separate layers;

[0036] (3) Take all the supernatant in a 2 ml centrifuge tube, centrifuge at 10,000 r / min for 10 s, and then layer;

[0037] (4) Take 1.5 ml of the supernatant in an industrial pigment purification tube, shake it for 20 s, let it stand for 30 s, then discard the supernatant;

[0038] (5) Add 0.5 ml methanol, shake for 20 s, let stand for 30 s, take 0...

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Abstract

The invention discloses a surface-enhanced Raman rapid detection method for rhodamine B in chili powder chili oil, and belongs to the technical field of food safety detection, and the method comprisesthe following steps: extracting a to-be-detected sample with a mixed solution of n-hexane and acetone, adsorbing and eluting with acidic aluminum oxide, removing capsochrome interference, uniformly mixing the obtained lower-layer to-be-detected clear liquid with a proper amount of a reinforcing reagent A and a reinforcing reagent B, and detecting with a Raman spectrometer. According to the detection method disclosed by the invention, an extraction reagent is improved, adsorption and desorption processes are newly added, the detection limit is obviously reduced, the minimum standard addition amount of a real sample can be detected to be 0.05 ppm, and the detection method is simple and convenient in sample pretreatment, small in dosage, rapid, simple and convenient, high in precision and low in cost.

Description

technical field [0001] The invention belongs to the technical field of food safety detection, and in particular relates to a surface-enhanced Raman rapid detection method for rhodamine B in chili powder and chili oil. Background technique [0002] Rhodamine B (Rhodamine B), also known as rose red B, or alkaline rhodamine, commonly known as flower pink, is a synthetic dye with a bright pink color. Rhodamine B has strong fluorescence in solution, and it is used as cell fluorescence staining agent in the laboratory, colored glass, special fireworks and firecrackers and other industries. It was once used as a food additive, but it was proved by experiments that rhodamine B can cause cancer, and it is not allowed to be used as food dyeing. [0003] The detection methods of rhodamine B in condiments used in existing reports include ultra-high performance liquid chromatography-tandem mass spectrometry, solid phase extraction-ultra high performance liquid chromatography-electrospra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/658
Inventor 常化仿马宁倪天瑞贾梦伟章祥尧伟峰
Owner OPTOTRACE SUZHOU TECH
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