Hepatic stellate cell targeted magnetic resonance molecular imaging agent capable of assessing liver fibrosis degree and preparation method of hepatic stellate cell targeted magnetic resonance molecular imaging agent

A technology for hepatic stellate cells and liver fibrosis, which is applied in the field of hepatic stellate cell-targeted magnetic resonance molecular imaging agents and its preparation, can solve the problems of changes, inconsistencies, and sample sampling that cannot be followed up accurately in patients with chronic liver diseases Errors in pathological observation and other problems, to achieve the effect of inhibiting clinical treatment ability and high sensitivity

Inactive Publication Date: 2020-07-14
HARBIN MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, liver biopsy is invasive and can cause corresponding complications, so it is not generally accepted by patients, which causes a large proportion of patients with chronic liver disease to delay diagnosis and treatment; at the same time, in asymptomatic patients, Difficulty repeating liver biopsies, which prevents accurate follow-up of changes in patients with chronic liver disease; in addition, sample sampling errors and inconsistencies in pathological observations affect the accuracy of liver biopsies

Method used

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  • Hepatic stellate cell targeted magnetic resonance molecular imaging agent capable of assessing liver fibrosis degree and preparation method of hepatic stellate cell targeted magnetic resonance molecular imaging agent
  • Hepatic stellate cell targeted magnetic resonance molecular imaging agent capable of assessing liver fibrosis degree and preparation method of hepatic stellate cell targeted magnetic resonance molecular imaging agent
  • Hepatic stellate cell targeted magnetic resonance molecular imaging agent capable of assessing liver fibrosis degree and preparation method of hepatic stellate cell targeted magnetic resonance molecular imaging agent

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Embodiment 1

[0025] 1) Disperse 0.01-10g iron acetylacetonate in 2-200mL oleylamine, add 0.1-200mL trioctylphosphine; the resulting mixture is heated to 120°C, reacted for 30 minutes, then continued to heat up to 300-350°C, reacted for 0.5 -10 hours; after the reaction is stopped, the reaction liquid is naturally cooled to room temperature, and then ethanol, isopropanol or acetone is added in a volume ratio of 1:3-5 for centrifugation, and the obtained precipitate is oil-soluble iron phosphide nanoparticles;

[0026] 2) Dissolve 0.56g of N,N-carbonyldiimidazole (CDI) in 20ml of dimethyl sulfoxide (DMSO), add 1g of retinol after complete dissolution, and stir at 30°C for 24 hours under dark conditions to obtain activation of retinol.

[0027] 3) Dissolve 0.1-500mg oil-soluble ferric phosphide nanoparticles in 1-50mL chloroform; dissolve 0.5-500mg retinol in 1-50mL chloroform; then fully mix the two solutions and place in a fume hood Stand still to make the liquid volatilize and disappear, ...

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Abstract

The present invention discloses a hepatic stellate cell targeted magnetic resonance molecular imaging agent capable of assessing liver fibrosis degree and a preparation method of the hepatic stellatecell targeted magnetic resonance molecular imaging agent, and belongs to the technical field of medicine and biological imaging. The hepatic stellate cell targeted magnetic resonance molecular imagingagent can specifically target hepatic stellate cells by coupling retinol molecules and AKT inhibitors to detect degree and development liver fibrosis process. The hepatic stellate cell targeted magnetic resonance molecular imaging agent is characterized in that the coupled retinol has a magnetic resonance imaging function, at the same time, the coupled AKT inhibitors have a function of treating liver fibrosis, and a purpose of integration of diagnosis and treatment is reached. The hepatic stellate cell targeted magnetic resonance molecular imaging agent is characterized by being high in imaging sensitivity.

Description

technical field [0001] The invention belongs to the technical field of medicine and biological imaging, and in particular relates to a hepatic stellate cell-targeted magnetic resonance molecular imaging agent capable of evaluating the degree of liver fibrosis and a preparation method thereof. Background technique [0002] Liver fibrosis and cirrhosis are common chronic progressive liver diseases, which are a repair response of the liver to chronic damage. Various cytokines secreted by damaged hepatocytes and activated Kupffer cells can lead to the activation of hepatic stellate cells (HSCs) into myofibroblasts, and the activated stellate cells synthesize and secrete a large amount of collagen-based extracellular The matrix is ​​deposited in the liver, leading to the generation of liver fibrosis, which can further develop into cirrhosis. [0003] Hepatic fibrosis is chronic injury leading to excessive deposition of extracellular matrix (ECM) in the liver, and hepatic stellat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/10A61K47/52A61K31/4375A61P1/16
CPCA61K49/10A61K47/52A61K31/4375A61P1/16
Inventor 周洋王凯黄圣航张子谦
Owner HARBIN MEDICAL UNIVERSITY
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