Application of rotundine in preparation of medicine for treating fatty liver

A fatty liver and drug technology, applied in the field of medicine, can solve problems such as discomfort and adverse reactions to the body, achieve good therapeutic effects, save development costs, and shorten the research and development cycle

Pending Publication Date: 2020-07-17
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, when the effect of lifestyle intervention in 3-6 months is not good, drug treatment for metabolic disorders is tried, and lipid-lowering drugs (such as statins) or liver-protecting drugs (such as silymarin, etc.) are generally used. The drug

Method used

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  • Application of rotundine in preparation of medicine for treating fatty liver
  • Application of rotundine in preparation of medicine for treating fatty liver
  • Application of rotundine in preparation of medicine for treating fatty liver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the effect of rotundine on the cellular lipid deposition model that sodium oleate induces L02 cell establishment

[0030] experimental method:

[0031] (1) Sodium oleate induces L02 cells to establish a cell lipid deposition model:

[0032] 1. Use tweezers to take out the clean glass slide soaked in alcohol, and put it into a 24-well plate after the alcohol evaporates;

[0033] 2. Human normal liver cells (L02 cells) in the logarithmic growth phase were digested from a culture dish with a diameter of 10 cm, and diluted to a density of 10 according to the cell density at 1:6-1:10. 5 cells / mL of cell suspension, inoculate the cell suspension on slides in a 24-well plate, add 800 μL of cell suspension to each well plate, add RPMI1640 complete medium containing 10% fetal bovine serum, and put in a cell culture incubator (37°C, 5% CO 2 ), wait for the cells to climb the sheet;

[0034] 3. After 24 hours of cell climbing, prepare sodium oleate mother solutio...

Embodiment 2

[0047] Embodiment 2, the effect of rotundine on the fatty liver rat model of SD rat high-fat feed feeding

[0048] experimental method:

[0049] (1) Establishment of SD rat model of fatty liver fed with high-fat diet

[0050] 1. 8-week-old SD male rats were weighed, randomly divided into five groups according to body weight, 8 rats in each group, and adaptively fed for 3 days.

[0051] 2. The rats in the control group (SD rats without modeling) were fed with common feed, the rats in the model group were fed with high-fat feed, and the rats in the high, medium and low dose groups of rotundin were fed with high-fat feed. The feeding model was established for 12 weeks, during which the food intake, water intake and body weight of the rats were recorded.

[0052] (2) Administration

[0053] 1. After successful modeling, a rotundine solution was prepared as a solvent with a mass concentration of 0.3% CMC-Na solution;

[0054] 2. The rats in the control group and the model group w...

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Abstract

The invention provides an application of rotundine in preparation of a medicine for treating fatty liver, and belongs to the field of medicines. The invention mainly provides application of a compoundshown as a formula I or salt thereof in preparation of the medicine for treating the fatty liver and/or lowering lipid. Researches find that the rotundine can significantly inhibit lipid deposition in the liver of a fatty liver patient, has a lipid lowering effect, a good treatment effect on the fatty liver, and no harm to the health of the fatty liver patient, and provides a novel method for treating the fatty liver; and meanwhile, the method is a novel use of the old medicine, can greatly save the development time and cost, is more detailed in listed pharmacokinetics and safety data, can achieve the second-stage clinical evaluation through the development of the novel use, shortens the research and development period, saves the development cost, and achieves the maximum utilization of resources.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the use of rotundine in the preparation of medicines for treating fatty liver. Background technique [0002] Fatty liver refers to the excessive accumulation of lipids in liver cells caused by various reasons, especially triglycerides. The total fat content of normal human liver accounts for 5% of the liver weight, and contains phospholipids, triacylglycerols, fatty acids, cholesterol and cholesteryl lipids. If the total fat exceeds 5% of the liver weight, it is called fatty liver. The more the total fat exceeds, the more severe the fatty liver will be. Fatty liver has become the number one chronic liver disease in my country. Alcoholic and non-alcoholic fatty liver disease are lifestyle-related chronic liver diseases, which require long-term comprehensive treatment combined with a reasonable diet, increased exercise, and a good attitude. At present, there is no specific dr...

Claims

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Application Information

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IPC IPC(8): A61K31/4375A61P1/16A61P3/06
CPCA61K31/4375A61P1/16A61P3/06
Inventor 张媛媛孙晓东
Owner SICHUAN UNIV
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