Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of rotundine in preparation of medicine for treating fatty liver

A fatty liver and drug technology, applied in the field of medicine, can solve problems such as discomfort and adverse reactions to the body, achieve good therapeutic effects, save development costs, and shorten the research and development cycle

Pending Publication Date: 2020-07-17
SICHUAN UNIV
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Under normal circumstances, when the effect of lifestyle intervention in 3-6 months is not good, drug treatment for metabolic disorders is tried, and lipid-lowering drugs (such as statins) or liver-protecting drugs (such as silymarin, etc.) are generally used. The drug itself has many adverse reactions, and when it is used for the treatment of fatty liver, the original purpose of the drug may also become a new adverse reaction that causes body discomfort
However, there is no report on the use of rotundine in the treatment of fatty liver

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of rotundine in preparation of medicine for treating fatty liver
  • Application of rotundine in preparation of medicine for treating fatty liver
  • Application of rotundine in preparation of medicine for treating fatty liver

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Embodiment 1, the effect of rotundine on the cellular lipid deposition model that sodium oleate induces L02 cell establishment

[0030] experimental method:

[0031] (1) Sodium oleate induces L02 cells to establish a cell lipid deposition model:

[0032] 1. Use tweezers to take out the clean glass slide soaked in alcohol, and put it into a 24-well plate after the alcohol evaporates;

[0033] 2. Human normal liver cells (L02 cells) in the logarithmic growth phase were digested from a culture dish with a diameter of 10 cm, and diluted to a density of 10 according to the cell density at 1:6-1:10. 5 cells / mL of cell suspension, inoculate the cell suspension on slides in a 24-well plate, add 800 μL of cell suspension to each well plate, add RPMI1640 complete medium containing 10% fetal bovine serum, and put in a cell culture incubator (37°C, 5% CO 2 ), wait for the cells to climb the sheet;

[0034] 3. After 24 hours of cell climbing, prepare sodium oleate mother solutio...

Embodiment 2

[0047] Embodiment 2, the effect of rotundine on the fatty liver rat model of SD rat high-fat feed feeding

[0048] experimental method:

[0049] (1) Establishment of SD rat model of fatty liver fed with high-fat diet

[0050] 1. 8-week-old SD male rats were weighed, randomly divided into five groups according to body weight, 8 rats in each group, and adaptively fed for 3 days.

[0051] 2. The rats in the control group (SD rats without modeling) were fed with common feed, the rats in the model group were fed with high-fat feed, and the rats in the high, medium and low dose groups of rotundin were fed with high-fat feed. The feeding model was established for 12 weeks, during which the food intake, water intake and body weight of the rats were recorded.

[0052] (2) Administration

[0053] 1. After successful modeling, a rotundine solution was prepared as a solvent with a mass concentration of 0.3% CMC-Na solution;

[0054] 2. The rats in the control group and the model group w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides an application of rotundine in preparation of a medicine for treating fatty liver, and belongs to the field of medicines. The invention mainly provides application of a compoundshown as a formula I or salt thereof in preparation of the medicine for treating the fatty liver and / or lowering lipid. Researches find that the rotundine can significantly inhibit lipid deposition in the liver of a fatty liver patient, has a lipid lowering effect, a good treatment effect on the fatty liver, and no harm to the health of the fatty liver patient, and provides a novel method for treating the fatty liver; and meanwhile, the method is a novel use of the old medicine, can greatly save the development time and cost, is more detailed in listed pharmacokinetics and safety data, can achieve the second-stage clinical evaluation through the development of the novel use, shortens the research and development period, saves the development cost, and achieves the maximum utilization of resources.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the use of rotundine in the preparation of medicines for treating fatty liver. Background technique [0002] Fatty liver refers to the excessive accumulation of lipids in liver cells caused by various reasons, especially triglycerides. The total fat content of normal human liver accounts for 5% of the liver weight, and contains phospholipids, triacylglycerols, fatty acids, cholesterol and cholesteryl lipids. If the total fat exceeds 5% of the liver weight, it is called fatty liver. The more the total fat exceeds, the more severe the fatty liver will be. Fatty liver has become the number one chronic liver disease in my country. Alcoholic and non-alcoholic fatty liver disease are lifestyle-related chronic liver diseases, which require long-term comprehensive treatment combined with a reasonable diet, increased exercise, and a good attitude. At present, there is no specific dr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/4375A61P1/16A61P3/06
CPCA61K31/4375A61P1/16A61P3/06
Inventor 张媛媛孙晓东
Owner SICHUAN UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com