Method for analyzing drug resistance of tumor cells
A technology of tumor cells and drug resistance, applied in the field of tumor treatment, can solve problems such as failure of tumor chemotherapy, and achieve simple and efficient results
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Embodiment 1
[0038] Dissolve 0.4g aminodextran in 25mL water and heat until dissolved. The temperature was then lowered to 30°C. Nitrogen gas was passed for 1 h, and dilute nitric acid solution containing 0.05 g of ceric ammonium nitrate was added under the protection of nitrogen gas. After 5 min, 0.06 mL of methyl acrylate was added. After 0.5h, add 0.012g of diallyl disulfide and react for 4h. After the reaction was completed, it was dialyzed at room temperature for 3 days to obtain aminodextran nanoparticles.
[0039]The aminodextran nanoparticles were dissolved in water, and the pH was adjusted to 8.5 with alkali to form an aqueous solution of aminodextran nanoparticles with a concentration of 8 mg / mL. The cyanine dye CY5.5 succinimide was dissolved in dimethyl sulfoxide to form a cyanine dye CY5.5 succinimide dimethyl sulfoxide solution (CY5.5-NHS solution) at a concentration of 10 mg / mL. The aminodextran nanoparticle solution and the CY5.5-NHS solution are mixed according to a ma...
Embodiment 2
[0041] Dissolve 1g of hydroxymethyl chitosan in 25mL of water and heat until dissolved. The temperature was lowered to 30°C. Nitrogen was passed for 1 hour, and dilute nitric acid solution containing 0.06 g of ceric ammonium nitrate was added under the protection of nitrogen. After 5 min, 0.05 mL of methyl acrylate was added. After 0.5h, add 0.012g of diallyl disulfide and react for 4h. After the reaction was completed, it was dialyzed for 3 days to obtain hydroxymethyl chitosan nanoparticles.
[0042] The hydroxymethyl chitosan nanoparticles were dissolved in water to form an aqueous solution of hydroxymethyl chitosan nanoparticles with a concentration of 5 mg / mL. The cyanine dye CY5.5 succinimide was dissolved in dimethyl sulfoxide to form a cyanine dye CY5.5 succinimide dimethyl sulfoxide solution (CY5.5-NHS solution) at a concentration of 10 mg / mL. The hydroxymethyl chitosan nano particle solution and the CY5.5-NHS solution are mixed according to a mixing ratio of 50-2...
Embodiment 3
[0044] Dissolve 0.5 g of dextran (molecular weight of 40,000 Da) and 0.5 g of chitosan in 25 mL of water and heat until dissolved. The temperature was lowered to 30°C. Nitrogen was blown for 1 hour, and dilute nitric acid solution containing 0.06 g of cerium ammonium nitrate was added under the protection of nitrogen, 0.05 mL of methyl acrylate was added after 5 minutes, 0.012 g of diallyl disulfide was added after 0.5 hours, and the reaction was carried out for 4 hours. After the reaction was completed, it was dialyzed for 3 days to obtain dextran / chitosan nanoparticles.
[0045] Dextran / chitosan nanoparticles were dissolved in water to form an aqueous solution of dextran / chitosan nanoparticles with a concentration of 10 mg / mL. Dissolve the graphene quantum dots in water to form an aqueous solution of graphene quantum dots with a concentration of 2 mg / mL. The dextran / chitosan nano particle aqueous solution and the graphene quantum dot aqueous solution are mixed according to...
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