High-androstenedione-producing recombinant mycobacterium and construction method and application of high-androstenedione-producing recombinant mycobacterium

A technology of androstenedione and mycobacteria, which is applied in the field of biotechnology and biochemical industry, can solve the problems of poor transformation ability of strains, influence on production efficiency, and easy degradation of steroids, so as to reduce the generation of by-products and improve production efficiency , The effect of simple process method
CN111454871AActive Publication Date: 2020-07-28TIANJIN UNIV
16 Cites 2 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2020-07-28

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The present invention discloses a high-androstenedione-producing recombinant mycobacterium and a construction method and an application of the high-androstenedione-producing recombinant mycobacterium.The construction method is as follows: cholesterol oxidase gene ChoM2, sterone C27 monooxygenase gene Smo2 carrying an RBS fragment and 17[beta]-hydroxysterol dehydrogenase gene Hsd4A carrying an RBSfragment are introduced into mycobacterium sp. CICC 21097 to obtain the high-androstenedione-producing recombinant mycobacterium MNCSH. A fermentation main product of the recombinant strain obtainedby the method is 4-androstenedione, the yield is 90% or more, the selectivity is 90% or more, formation of by-products is reduced and the yield of the androstenedione is increased. Compared with an original strain, the recombinant strain has better catalytic conversion performance and can improve production efficiency, and the processing method is simple and efficient.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the fields of biotechnology and biochemical industry, and specifically relates to a recombinant mycobacterium with high androstenedione production, a construction method and application thereof. Background technique

[0002] Steroid hormone drugs refer to hormone drugs with a steroid structure in their molecular structure, which is an important class of clinically important drugs, mainly including adrenocortical hormones and sex hormones. Steroid hormone drugs are widely used in the treatment of various diseases such as rheumatism, cardiovascular disease, cancer, skin disease and endocrine disorders due to their numerous pharmacological effects, such as anti-infection, anti-allergy, anti-virus and anti-shock Wait. After decades of research and development, steroid hormone drugs have formed a large class of steroid drugs with various types, wide clinical applications and strong demand, and have become the second largest drug af...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More