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SERS labeled nanoprobe with gold-hexanethiol-mesoporous silicon structure, and preparation method and application thereof

A nanoprobe, hexanethiol technology, applied in nanotechnology, nanotechnology, nanotechnology and other directions for materials and surface science, can solve the problems of difficult pH accurate monitoring, complicated operation, photobleaching, etc. , the detection process is simple, the effect of high sensitivity

Pending Publication Date: 2020-07-31
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods for monitoring cell pH mainly include fluorescence method, etc., but these methods have certain limitations due to their own characteristics, such as toxicity, photobleaching, complicated operation, etc. Therefore, it is of great significance to establish a fast, simple and accurate detection method

Method used

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  • SERS labeled nanoprobe with gold-hexanethiol-mesoporous silicon structure, and preparation method and application thereof
  • SERS labeled nanoprobe with gold-hexanethiol-mesoporous silicon structure, and preparation method and application thereof
  • SERS labeled nanoprobe with gold-hexanethiol-mesoporous silicon structure, and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0068] (1) Preparation of gold nanoparticles

[0069] Under constant stirring, 100 mL of 1 mM chloroauric acid (HAuCl 4 ) solution was heated to boiling, and then 6 mL of 38.8 mM trisodium citrate aqueous solution was added. At this time, the color change of the solution is: light yellow-colorless-black-purple-dark red, wait for the solution to turn dark red and continue to heat and reflux for 15-20min. Finally, cool to normal temperature to prepare 30nm gold nanoparticles, see figure 2 a.

[0070] (2) Preparation of 4-MBA-labeled gold nanoparticles modified by hexanethiol

[0071] Take the above 1mL 1mmol / L gold nanoparticles, add 5μL 100μM hexanethiol solution to react for 30 minutes, centrifuge (6000rpm, 12min) after the reaction, resuspend with 1mL boric acid buffer, and react with 30μL 100mM 4-MBA for 30min After the reaction, centrifuge (8000rpm, 10min), and then resuspend in 1mL boric acid buffer to obtain gold nanoparticles covered with Raman signal molecules, tha...

Embodiment 2

[0086] Take 1 mL of the prepared gold nanoparticle solution, put it into an EP tube, add 30 μL of 100 μM 4-MBA, react at room temperature for 30 minutes, centrifuge at 8000 rpm, remove the supernatant, and resuspend with 1 mL boric acid buffer to prepare SERS-labeled naked Gold nanoparticles (referred to as Au-MBA), the prepared nanoparticles and the gold nanoparticles wrapped by mesoporous silicon in step (4) of Example 1 (referred to as Au-MBA-HT-Si) are placed at the same time The repetitive diagram of Figure 4 Shown; the time stability diagram is shown in Figure 5 shown. according to Figure 4 and Figure 5 It can be seen that gold nanoparticles wrapped with mesoporous silicon have better reusability and time stability than SERS-labeled bare gold nanoparticles, and are more suitable for SERS-labeled nanoprobes.

[0087] Therefore, the mesoporous silicon-wrapped gold nanoparticles prepared by the present invention have the following obvious advantages: (1) the hexanet...

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Abstract

The invention discloses an SERS labeled nanoprobe with a gold-hexanethiol-mesoporous silicon structure, and a preparation method and application thereof. According to the preparation method, introducing hexanethiol and mesoporous silicon into the surfaces of gold nanoparticles marked by 4-MBA to form an SERS marked nanoprobe with a gold-hexanethiol-mesoporous silicon structure, and fixing signal molecules by utilizing molecular intervals formed by hexanethiol so as to weaken thermotactic motion of the signal molecules under laser thermal induction, so that a more stable Raman signal is formed;the coated mesoporous silicon forming a structure similar to a molecular sieve, so that the influence of biomacromolecules in cells on signal molecules is avoided, and the stability and accuracy of Raman signals are further improved. The nanoprobe disclosed by the invention can be massively prepared in advance. During operation, only the nanoprobe needs to be introduced into cancer cells for co-mixed culture, and detection can be immediately carried out by washing after completion: the collection time is 1s, and then the pH value is read out from a standard curve, so that rapid quantitative detection is realized. The nanoprobe can be applied to clinical diagnosis and screening of early-stage cancers.

Description

technical field [0001] The invention belongs to the technical field of biological detection, in particular to a gold-hexanethiol-mesoporous silicon-structured SERS-labeled nanoprobe and its preparation method and application, in particular to a novel SERS substrate monitoring cell based on mesoporous silicon-wrapped pH method. Background technique [0002] As an important reference for the change of acid-base in the microenvironment in the body, the pH value of the cell can reflect the physiological health status, affect the physiological and pathological activities of the human body, and is closely related to the occurrence and development of tumors. It is a hot research topic at present. At present, the methods for monitoring cell pH mainly include fluorescence method, etc., but these methods have certain limitations due to their own characteristics, such as toxicity, photobleaching, complicated operation, etc. Therefore, it is of great significance to establish a fast, s...

Claims

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Application Information

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IPC IPC(8): G01N21/65G01N33/58B22F9/24B22F1/00B82Y30/00B82Y40/00
CPCG01N21/658G01N33/587B22F9/24B82Y30/00B82Y40/00B22F1/054
Inventor 胡勇军张文建江宁静王棋庄秀眉王俊杰余星星
Owner SOUTH CHINA NORMAL UNIVERSITY
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