Cosmetic composition comprising styphnolobium japonicum fruit fermented solution or extract liquid thereof as effective ingredient for reducing skin wrinkle and preparation method therefor
A technology for cosmetic compositions and active ingredients, applied in the field of preparation methods, can solve the problems of poor skin absorption, insignificant effects, and difficulty in obtaining substantial effects, etc., and achieve the effects of promoting fibroblast proliferation, improving wrinkles and skin wrinkles. Effects without side effects
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[0067] Hereinafter, the preferred implementation of the present invention will be described with reference to the accompanying drawings. Preferably, the preparation method of the cosmetic composition for improving skin wrinkles comprising pagoda tree fruit fermentation liquid as an active ingredient of the present invention includes the following steps, but is not limited here.
[0068] The present invention is characterized in that it comprises step (S10), obtaining the fruit extract of Sophora japonica; step (S20), inoculating the Lactobacillus strain in the fruit extract of Sophora japonica to obtain the fermentation liquid; step (S30), obtaining the extract from the above steps (S20) after removing the culture bacteria in the pagoda tree fruit fermented liquid, utilize the water-bath extraction method to obtain pagoda tree fruit fermented extract; ) in the Sophora japonica fruit fermented extract to obtain a cosmetic composition by adding stabilizers, vitamins, pigments, p...
preparation example
[0083] Preparation example. Preparation method of Sophora japonica fruit extract
[0084] The pagoda tree fruit was washed with purified water, dried, and then pulverized to obtain a fine pulverized product through a 100-mesh sieve. Add 10% (V / V) ethanol aqueous solution so that the concentration of pagoda tree fruit pulverization becomes 100g / L, under water-bath condition, carry out reflux extraction 3 times, carry out 5 hours each time, after cold soaking, utilize 6 μm Filter paper (Whatman #3) was used to prepare an extract, which was used as the extract for the fermentation process.
[0085] In addition, the extract was concentrated under reduced pressure and freeze-dried at 50° C. or lower, and used for efficacy evaluation.
Embodiment
[0086] Example. The preparation method of pagoda tree fruit fermentation liquid and pagoda tree fruit fermentation extract
[0087] Add Lactobacillus rhamnosus (Lactobacillusrhamnosus) vitaP1 strain (preservation number: KACC92054P) and LB broth (LB Broth) medium to the Sophora japonica fruit extract prepared in the above-mentioned preparation example, and under 30 ℃ of aerobic conditions , fermented for 96 hours. After culturing, the culture solution was centrifuged to remove the culture bacteria for the first time to obtain a filtrate, and the above-mentioned filtrate was filtered to 0.2 μm by a filter again, and then sterilized (121, 15 minutes, 1.5 pressure), to No longer cultured. Purified water was added to the fermentation product of pagoda tree fruit obtained by sterilization, and the reflux water bath extraction was carried out 3 times for 5 hours each time, and after cold soaking, it was filtered with 6 μm filter paper (what man #3) . The extract obtained by fil...
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