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PRP preparing device and PRP preparing method

A technology for preparing devices and centrifuge tubes, which is applied to chemical instruments and methods, semi-permeable membrane separation, centrifuges, etc., can solve the problems of high risk of secondary centrifugal infection, time-consuming, joint swelling, etc., to reduce the risk of pollution, The effect of reducing the total amount of blood drawn and increasing the concentration

Pending Publication Date: 2020-08-07
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to ensure the effective concentration, the second centrifugation method is mainly used, but it will take a lot of time to wait for the preparation. At the same time, the second centrifugation has a greater risk of infection. In the separation process, in order to collect as many platelets as possible, a large number of white blood cells will be collected at the same time. , for some diseases and repairs, such as osteoarthritis, cartilage regeneration and bone regeneration, it can cause early joint swelling after injection, aggravate pain, and reduce curative effect in the later stage

Method used

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Examples

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Embodiment 1

[0048] A PRP preparation device that can obtain high-concentration PRP more quickly, reduce the risk of contamination and preparation time, reduce the total amount of blood drawn by patients, reduce blood waste, and solve the problem of filtering out red blood cells while avoiding red blood cells covering the filter membrane, such as figure 1 , figure 2 As shown, specifically set to the following structure:

[0049] Basically, it includes a sterile protective cover 1 and a filter membrane 2 .

[0050] The aseptic protection cover 1 is roughly a dome casing, and an accommodating space is formed on its inner side. In this embodiment, in order to facilitate the observation of the filtration process, the aseptic protection cover 1 is a sterile transparent plastic shell or a sterile clear plastic film for easy and timely disposal if filtration problems are observed. The top center of the sterile protective cover 1 is provided with an extension tube, and the end of the extension ...

Embodiment 2

[0056] This embodiment is further optimized on the basis of the above-mentioned embodiments, further to better realize the present invention, especially adopt the following configuration structure:

[0057] The PRP preparation device also includes a collection funnel 5, the centrifuge tube connection port 4 of the sterile protective cover 1 is sealed and connected with the collection funnel 5, and the centrifuge tube connection port 4 of the sterile protective cover 1 can be directly sealed and connected to the drain of the collection funnel 5. The inlet port can also wrap the collection funnel 5 and be sealed and connected with the discharge port of the collection funnel 5, and make the centrifuge tube connection port 4 communicate with the discharge end of the collection funnel 5. The collection funnel 5 is conducive to collecting the filtrate, which can accelerate Filtrate collection. A sterile cap is installed at the discharge end of the collection funnel 5, and the discha...

Embodiment 3

[0060] This example provides a method for preparing PRP. This PRP preparation method uses blood collection tubes, centrifuge tubes, centrifuges and the PRP preparation device in Example 2 to prepare PRP, which can obtain high-concentration PRP more quickly and reduce the risk of contamination. Compared with the preparation time, reducing the total amount of blood drawn by patients, reducing blood waste and solving the problem of filtering red blood cells while avoiding red blood cells covering the filter membrane, the PRP preparation method includes the following steps:

[0061] Step S1: Connect the centrifuge tube to the centrifuge tube connection port of the sterile protective cover.

[0062] After step S1 is completed, proceed to step S2: collect the patient's own venous blood in a blood collection tube under aseptic conditions, wherein the blood volume in the blood collection tube is controlled at 20-30ml.

[0063] After step S2 is completed, proceed to step S3: remove the...

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Abstract

The invention provides a PRP preparing device and a PRP preparing method, and relates to the technical field of PRP preparation. The problems that the PRP preparation time is long, the contamination risk is high and the total blood drawing volume of a patient is high in the prior art are solved. The PRP preparing device comprises a sterile protection cover and a filtration membrane arranged in thesterile protection cover. The sterile protection cover is provided with a blood sampling tube connecting port and a centrifugal tube connecting port. The circumference of the filtration membrane is connected with the inner wall of the sterile protection cover. The filtration membrane is located between the blood sampling tube connecting port and the centrifugal tube connecting port. The filtration membrane comprises at least one single-layer polyester fiber non-woven fabric without charges. The filtration aperture of the filtration membrane is smaller than or equal to 7 [mu]m. In the method,PRP is prepared through a sampling tube, a centrifugal tube, a centrifugal machine and the PRP preparing device, high-concentration PRP can be more rapidly obtained, the contamination risk is reduced,the preparation time is shortened, the total blood drawing volume of the patient is reduced, blood waste is reduced, and the purpose that red blood cells are prevented from covering a filtration membrane when being filtered out is realized.

Description

technical field [0001] The invention relates to the technical field of PRP preparation, in particular to a PRP preparation device and a preparation method. Background technique [0002] PRP (Platelet-rich plasma) is a plasma component containing a large number of platelets obtained by centrifuging and extracting autologous blood, and the platelet content contained in it is significantly higher than that in normal human plasma. [0003] Platelets are very small blood cells with a diameter of less than 2 μm, usually invisible to the naked eye, and the content of platelets in normal human blood is at least 100,000 to 150,000 platelets per 1 μl. According to the content of white blood cells and fibrin in PRP, it can be divided into various types, such as leukocyte-reducing PRP, leukocyte-rich PRP, defibrin PRP and platelet-rich fibrin PRP. Growth factors (growth factors, GFs) in PRP are very rich, mainly produced by α-granules released by activated platelets, such as vascular e...

Claims

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Application Information

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IPC IPC(8): B04B5/10B01D61/00
CPCB04B5/10B01D61/00
Inventor 余曦向小娜蒋红英刘岩何红晨
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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