Preparation of carbon quantum dot fluorescent probe and application of carbon quantum dot fluorescent probe in selective detection of active oxygen

A carbon quantum dot and fluorescent probe technology, which is applied in the field of rapid preparation of carbon quantum dot fluorescent probes, can solve the problems of biological hazards and cumbersome modification process

Pending Publication Date: 2020-08-07
EAST CHINA UNIV OF SCI & TECH
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These organic molecules are potentially harmful t...

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  • Preparation of carbon quantum dot fluorescent probe and application of carbon quantum dot fluorescent probe in selective detection of active oxygen
  • Preparation of carbon quantum dot fluorescent probe and application of carbon quantum dot fluorescent probe in selective detection of active oxygen
  • Preparation of carbon quantum dot fluorescent probe and application of carbon quantum dot fluorescent probe in selective detection of active oxygen

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Embodiment Construction

[0033] Preparation of a carbon quantum dot fluorescent probe and its application in selective detection of active oxygen:

[0034] (1) Synthesis of carbon quantum dots

[0035] All kinds of carbon quantum dots are obtained by one-step hydrothermal reaction. Specifically, 0.67 g of carbon source was ultrasonically dissolved in 5 mL of ultrapure water, followed by the addition of 0.185 mL of ethylenediamine. The above solution was transferred to a glass autoclave with a capacity of 25 mL, sealed and heated to 120° C. in an oven for 5 hours. After the reaction, cool to room temperature, adjust the pH to neutral with hydrochloric acid solution or sodium hydroxide solution, and further perform dialysis and filtration.

[0036]During the dialysis process, a dialysis bag with a molecular cut-off of 500 Da was used to dialyze in ultrapure water for 12 hours to remove small molecular impurities. After dialysis, the product is suction-filtered with a filter membrane with a diameter o...

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Abstract

The invention belongs to the technical field of nanomechanics and molecular biology, and relates to preparation of a carbon quantum dot fluorescent probe and application of the carbon quantum dot fluorescent probe in selective detection of active oxygen. Carbon quantum dots with specific detection functions on different active oxygen can be prepared by the method. A series of different carbon sources are used as precursors, and different carbon quantum dots are synthesized through a one-step hydrothermal reaction. XPS characterization shows that the types and contents of oxygen-containing groups and nitrogen-containing groups on the surfaces of the carbon quantum dots generated by different carbon sources are different, and various groups have different binding capacities with active oxygen such as superoxide anions (O2<.->) and hydroxyl radicals (.OH), so that the carbon quantum dots can be used for specific detection of related active oxygen. Meanwhile, an MTT method proves that thecarbon quantum dot synthesized by the method has good biocompatibility, can be used for in-vivo cell imaging and online detection, detects the content of active oxygen generated by stimulating livingcells by a drug, and is used for related cell counting.

Description

technical field [0001] The invention belongs to the technical fields of nanomaterials and molecular biology, and specifically relates to a method for rapidly preparing a carbon quantum dot fluorescent probe and effectively regulating the type and content of its surface functional groups, and the prepared carbon quantum dots are free from superoxide anion and hydroxyl groups. Based on the specific detection of various reactive oxygen species and the application in intracellular imaging. Background technique [0002] Active oxygen is a by-product of oxygen metabolism in organisms. Excessive active oxygen can affect the DNA methylation process and intracellular signal transduction, and change the expression of related genes, thereby inducing major diseases such as cancer and cardiovascular disease. On the other hand, compared with normal cells, the content of reactive oxygen species released by cancer cells is higher. Therefore, the detection of various reactive oxygen species ...

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/6428G01N21/643G01N21/6458G01N2021/6432Y02A50/30
Inventor 蓝闽波蔡璇赵红莉孙文倩张欢欢陈利娟陈开茶
Owner EAST CHINA UNIV OF SCI & TECH
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