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Bacillus subtilis, bacterial fertilizer as well as preparation method and application of bacterial fertilizer

A technology of Bacillus subtilis and Bacillus amylogenus, applied in the direction of botany equipment and method, biochemical equipment and method, application, etc., can solve the problems that the content of coliform group and the total number of bacterial colonies cannot be reduced, so as to inhibit growth and improve Lettuce Yield and Effect of Reducing Lettuce Diseases

Active Publication Date: 2020-08-14
BEIJING UNIV OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Traditional bacterial fertilizer, such as CN201710124044.9 discloses a special microbial selenium-enriched bacterial fertilizer for lettuce and its preparation method. The bacterial fertilizer provided by it can increase the selenium content of lettuce, inhibit fungal diseases, and increase the yield of lettuce, but it cannot Reducing the content of coliform bacteria and the total number of bacterial colonies carried by lettuce

Method used

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  • Bacillus subtilis, bacterial fertilizer as well as preparation method and application of bacterial fertilizer
  • Bacillus subtilis, bacterial fertilizer as well as preparation method and application of bacterial fertilizer
  • Bacillus subtilis, bacterial fertilizer as well as preparation method and application of bacterial fertilizer

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preparation example Construction

[0033] The present invention provides the preparation method of bacterial fertilizer described in above-mentioned technical scheme, comprises the following steps:

[0034] (1) inoculating Bacillus amyloliquefaciens Y1 into a liquid medium and culturing it to obtain a liquid fermentation broth of Bacillus amyloliquefaciens Y1;

[0035] (2) inoculating Bacillus subtilis Y2 in a liquid medium and culturing it to obtain a liquid fermentation broth of Bacillus subtilis Y2;

[0036] (3) mixing Bacillus amyloliquefaciens Y1 liquid fermentation liquid and Bacillus subtilis Y2 liquid fermentation liquid to obtain bacterial fertilizer;

[0037] The steps (1) and (2) are not in sequence.

[0038] In the present invention, bacillus amyloliquefaciens Y1 is inoculated and cultivated in a liquid medium to obtain a liquid fermentation liquid of bacillus amyloliquefaciens Y1. In the present invention, the temperature of the cultivation is preferably 37-40°C, more preferably 38-39°C; the time...

Embodiment 1

[0049] (1) Stretch Bacillus amyloliquefaciens Y1 with an inoculation loop and culture it on a nutrient agar plate for 12 hours, then take a single colony and insert it into 10 mL (20% Erlenmeyer flask liquid volume) liquid medium, and place it at 40°C and 150r Cultivate under the condition of 12h / min to obtain seed solution; mix 3.5mL seed solution with 175mL liquid medium in a volume ratio of 2:100, and cultivate for 40h at a temperature of 40°C and 150r / min to obtain the Bacillus amyloliquefaciens Y1 liquid fermentation broth; the liquid medium is 10g / L starch, 4g / L calcium chloride, 4g / L yeast extract, 4g / L tryptone, 2g / L urea, pH=7;

[0050] (2) the cultivation method and the condition of bacillus subtilis Y2 are identical with the cultivation method and the condition of bacillus amyloliquefaciens Y1 in the step (1), the difference is that the liquid medium is 10g / L starch, 4g / L calcium chloride, 4g / L yeast extract, 4g / L tryptone, 2g / L ammonium chloride, pH=7;

[0051] (3...

Embodiment 2

[0069] (1) Stretch Bacillus amyloliquefaciens Y1 with an inoculation loop and culture it on a nutrient agar plate for 12 hours, then take a single colony and insert it into 10 mL (20% Erlenmeyer flask liquid volume) liquid medium, and place it at 40°C and 150r Cultivate under the condition of 10h / min to obtain seed solution; mix 3.5mL seed solution with 175mL liquid medium in a volume ratio of 2:100, and cultivate for 40h at a temperature of 40°C and 150r / min to obtain the Bacillus amyloliquefaciens Y1 liquid fermentation broth; the liquid medium is 8g / L starch, 2g / L calcium chloride, 2g / L yeast extract, 2g / L tryptone, 1.5g / L urea, pH=6.5;

[0070] (2) the cultivation method and condition of bacillus subtilis Y2 are identical with the cultivation method and condition of bacillus amyloliquefaciens Y1 in step (1), the difference is that the liquid medium is 8g / L starch, 2g / L calcium chloride, 2g / L yeast extract, 2g / L tryptone, 1.5g / L ammonium chloride, pH=6.5;

[0071] (3) The ...

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Abstract

The invention belongs to the technical field of microbial fertilizer, and particularly relates to Bacillus subtilis, bacterial fertilizer as well as a preparation method and application of the bacterial fertilizer. According to the Bacillus subtilis Y2 provided by the invention, the preservation number is CGMCC NO.18752, and the Bacillus subtilis Y2 has an inhibiting effect on coliform bacteria and total bacterial count of bacteria in lettuce. According to the bacterial fertilizer containing the Bacillus subtilis, not only can yield increase and disease reduction of the lettuce be realized, but also the content of the coliform bacteria carried by the lettuce and the total bacterial count of bacteria of the lettuce can be reduced.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers, and in particular relates to a strain of Bacillus subtilis, bacterial fertilizers and a preparation method and application thereof. Background technique [0002] Lettuce, also known as leaf lettuce (Lactuca sativa), originated in Europe and was first introduced into my country from the southeast coastal areas. As consumers' demand for fresh lettuce increases, the planting of lettuce increases. In 2015, the total output of leafy vegetables accounted for 39.5% of the total vegetable output. In order to increase the yield of lettuce, excessive use of chemical fertilizers has resulted in excess soil nitrogen, decreased fertility, and imbalanced flora. At the same time, during the production process of farmers, coliform bacteria and harmful microorganisms can adhere to the surface of lettuce through irrigation water (fertilizer), animal manure or artificial pollution, and use the lost l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05G3/60C05G3/80A01N63/22A01P1/00A01P21/00C12R1/125C12R1/07
CPCC12N1/20C05G3/60C05G3/80A01N63/22C12R2001/125C12N1/205
Inventor 高秀芝高逸陈湘宁刘慧君刘慧徐全明钟连全姜奕冰
Owner BEIJING UNIV OF AGRI