Kit and method for rapidly detecting total amount of ethylene oxide and 2-chloroethanol in medical instrument

A technology of ethylene oxide and medical equipment, which is applied in the field of chemical detection, can solve the problems of not realizing the simultaneous detection of ethylene oxide and 2-chloroethanol, and can not meet the demand, and achieve fast speed, easy operation and high sensitivity Effect

Pending Publication Date: 2020-08-14
陕西省食品药品监督检验研究院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, first of all, the above-mentioned technology does not realize the simultaneous detection of ethylene oxide and 2-chloroethanol; secondly, the above-mentioned technology uses acetylacetone for color development, while this technology uses 4-amino-3-hydrazine-5-mercapto-1,2 , 4-triazine (AHMT) color development; moreover, according to its instruction manual, through technical reproduction, according to its method for detection, the shortest time-consuming is about 140min
This is undoubtedly unable to meet the demand for solving the technical problem of rapid detection.

Method used

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  • Kit and method for rapidly detecting total amount of ethylene oxide and 2-chloroethanol in medical instrument
  • Kit and method for rapidly detecting total amount of ethylene oxide and 2-chloroethanol in medical instrument
  • Kit and method for rapidly detecting total amount of ethylene oxide and 2-chloroethanol in medical instrument

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 reagent preparation and standard color scale solution and standard color card making

[0050] 1. Reagent preparation

[0051] The sodium hydroxide solution solution (reagent A) that configuration concentration is 0.1mol / L respectively, the potassium iodide solution (reagent B) that concentration is 0.2mol / L, the periodic acid and hydrochloric acid mixing that concentration is respectively 10g / L and 6mol / L Solution (reagent C), the sodium thiosulfate solution solution (reagent D) that concentration is 40g / L, the disodium edetate solution (reagent E) that concentration is 100g / L, the 4- Amino-3-hydrazine-5-mercapto-1,2,4-triazolene solution (reagent F), potassium periodate solution with a concentration of 15 g / L (reagent G).

[0052] 2. Preparation method of standard color scale solution and standard color comparison card

[0053] Use reagent A as the constant volume solution to prepare 2-chloroethanol standard work with concentrations of 0 μmol / mL, 0.005 μ...

Embodiment 2

[0056] Embodiment 2: the rapid detection example of oxirane and 2-chloroethanol total amount

[0057] The reagents used in this example were prepared in Example 1.

[0058] 1. Detection method

[0059] Step 1, preparation of sample liquid;

[0060] Accurately weigh 1.0g±0.01g of the sample to be tested and place it in a 50mL centrifuge tube, add 20mL of reagent A, add 3 drops of reagent B (about 150μL), place in a water bath at 60°C for 20min, take 2mL of the supernatant and centrifuge in 15mL In the tube, add 3 drops of reagent C (about 150 μL), cover the lid, shake well, and place it in a water bath at 60°C for 5 minutes, add reagent D dropwise until the yellow color just disappears, add 8 drops of reagent E (about 150 μL) and 8 drops of reagent F (about 150 μL), vortexed to mix, and then let stand for 10 minutes, then added 3 drops of reagent G (about 150 μL), vortexed to mix, and let stand for 5 minutes.

[0061] Step 2, colorimetric determination

[0062] After the re...

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Abstract

The invention provides a kit and a method for rapidly detecting the total amount of ethylene oxide and 2-chloroethanol in a medical instrument. The kit comprises a sodium hydroxide solution, a potassium iodide solution, a mixed solution of periodic acid and hydrochloric acid, a sodium thiosulfate solution, an ethylenediamine tetraacetic acid disodium solution, a 4-amino-3-hydrazino-5-mercapto-1,2,4-triazol solution, a potassium periodate solution and a standard colorimetric card. The method is simple and convenient to operate, low in cost, high in speed and high in sensitivity, can be used forrapidly and simultaneously detecting the residual quantity of ethylene oxide and 2-chloroethanol in a medical instrument on site, can obtain an experimental result within 40 minutes without using special instruments and equipment, and is suitable for rapid detection on site.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to the field of testing or analyzing materials by means of measuring their chemical or physical properties, and more specifically to the rapid detection of the total amount of residual ethylene oxide and 2-chloroethanol. Background technique [0002] Ethylene oxide (EO) is a broad-spectrum low-temperature sterilant, and its sterilization mechanism is that ethylene oxide reacts with carboxylic acids, amino groups, sulfur groups and hydroxyl groups on microbial proteins to produce an alkylation reaction to prevent microbial growth. Normal function, kills microorganisms. It can kill various microorganisms at room temperature and is widely used in the disinfection of medical devices. EO has strong penetrability and can penetrate micropores to reach the corresponding depth inside the product, thereby greatly improving the sterilization effect. However, while killing microorg...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/29
CPCG01N21/78G01N21/293
Inventor 刘海静李涛杨若朦王建山林芳赵俊楠马元斌丁慧王松袁磊王一欣贾丽华马鹏飞裴小龙
Owner 陕西省食品药品监督检验研究院
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