Kit and method for rapidly detecting total amount of ethylene oxide and 2-chloroethanol in medical instrument
A technology of ethylene oxide and medical equipment, which is applied in the field of chemical detection, can solve the problems of not realizing the simultaneous detection of ethylene oxide and 2-chloroethanol, and can not meet the demand, and achieve fast speed, easy operation and high sensitivity Effect
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Embodiment 1
[0049] Embodiment 1 reagent preparation and standard color scale solution and standard color card making
[0050] 1. Reagent preparation
[0051] The sodium hydroxide solution solution (reagent A) that configuration concentration is 0.1mol / L respectively, the potassium iodide solution (reagent B) that concentration is 0.2mol / L, the periodic acid and hydrochloric acid mixing that concentration is respectively 10g / L and 6mol / L Solution (reagent C), the sodium thiosulfate solution solution (reagent D) that concentration is 40g / L, the disodium edetate solution (reagent E) that concentration is 100g / L, the 4- Amino-3-hydrazine-5-mercapto-1,2,4-triazolene solution (reagent F), potassium periodate solution with a concentration of 15 g / L (reagent G).
[0052] 2. Preparation method of standard color scale solution and standard color comparison card
[0053] Use reagent A as the constant volume solution to prepare 2-chloroethanol standard work with concentrations of 0 μmol / mL, 0.005 μ...
Embodiment 2
[0056] Embodiment 2: the rapid detection example of oxirane and 2-chloroethanol total amount
[0057] The reagents used in this example were prepared in Example 1.
[0058] 1. Detection method
[0059] Step 1, preparation of sample liquid;
[0060] Accurately weigh 1.0g±0.01g of the sample to be tested and place it in a 50mL centrifuge tube, add 20mL of reagent A, add 3 drops of reagent B (about 150μL), place in a water bath at 60°C for 20min, take 2mL of the supernatant and centrifuge in 15mL In the tube, add 3 drops of reagent C (about 150 μL), cover the lid, shake well, and place it in a water bath at 60°C for 5 minutes, add reagent D dropwise until the yellow color just disappears, add 8 drops of reagent E (about 150 μL) and 8 drops of reagent F (about 150 μL), vortexed to mix, and then let stand for 10 minutes, then added 3 drops of reagent G (about 150 μL), vortexed to mix, and let stand for 5 minutes.
[0061] Step 2, colorimetric determination
[0062] After the re...
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