Hydroxyapatite-coated magnetic drug-loaded nanoparticles, preparation method thereof and application of hydroxyapatite-coated magnetic drug-loaded nanoparticles in preparation of osteosarcoma phototherapy drugs

Abstract

The invention discloses hydroxyapatite-coated magnetic drug-loaded nanoparticles as well as a preparation method and application thereof in preparation of osteosarcoma phototherapy drugs. The hydroxyapatite-coated magnetic drug-loaded nanoparticle comprises a polydopamine magnetic nanoparticle core, and the outer side of the polydopamine magnetic nanoparticle core is sequentially coated with a drug layer and a hydroxyapatite layer; the hydroxyapatite layer is adopted to completely wrap the drug layer, loaded drugs such as a photosensitizer cannot be released in advance, and the drug targetingeffect of the magnetic drug-loaded nanoparticles is accurate and effective; the photosensitizer and other drugs do not generate optical toxic and side effects on normal tissues; hydroxyapatite can behydrolyzed in the tumor environment with the pH being about 5, then a large amount of photosensitizer is released and retained in the tumor, it is more suitable for in-vivo application. Hydroxyapatitecan promote osteoblast proliferation, kill tumor cells, sterilize and promote bone tissue repair at the same time, and three purposes are achieved at a time.

Description

technical field [0001] The invention belongs to the technical field of photodynamic therapy, and in particular relates to a hydroxyapatite-wrapped magnetic drug-carrying nanoparticle, a preparation method thereof, and an application in preparing osteosarcoma phototherapy drugs. Background technique [0002] Osteosarcoma is a common malignant bone tumor with a high incidence rate, which usually occurs in adolescents aged 10-20. The currently recognized main treatment for osteosarcoma is comprehensive treatment based on high-dose chemotherapy and surgery. Under this comprehensive treatment, the 5-year survival rate of osteosarcoma patients in my country has increased from less than 20% to 55% now. %-75%. However, the side effects of chemotherapy are obvious, the most common ones are bone marrow suppression and severe digestive system lesions. Therefore, there is a need for a method with good antitumor effect and small side effects to treat osteosarcoma. [0003] Photodynamic...

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Problems solved by technology

[0005] The deficiencies of this calcium carbonate-wrapped polydopamine drug-loaded magnetic nanoparticles and its preparation method are: (1) when ICG and calcium carbonate are adsorbed onto Fe 3 o 4 On the surface of @PDA nanoparticles, the photosensitizer ICG and Ca 2+ , CO 3 2- At the same time added to Fe 3 o 4 @PDA nanoparticle suspension, which makes calcium carbonate and ICG in the same radial direction of the magnetic nanoparticles, in the process of guiding the magnetic nanoparticles to the target, the calcium carbonate will be more or less hydrolyzed, and also That is, before the magnetic nanoparticles reach the target, ICG is released, which leads to poor targeting of ICG by magnetic nanoparticles, and ICG still partially remains in normal tissues, causing phototoxic side effects on this part of normal tissues; (2) When the calcium carbonate-coated polydopamine drug-loaded magnetic nanoparticles are used in the treatment of osteosarcoma, their effect on promoting the differentiation of osteoblasts is not obvious

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