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Method for rapidly detecting varicella virus titer by using fluorescence method

A fluorescence detection and chickenpox technology, which is applied in rapid detection of varicella-zoster virus titer, varicella-zoster virus titer detection, high-throughput field, can solve the problems of shortened detection time, achieve fast detection speed, reduce The effect of human judgment error and high degree of automation

Active Publication Date: 2020-08-25
CHANGCHUN KEYGEN BIOLOGICAL PROD
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Problems solved by technology

[0010] During the research of VZV virus-related antibodies, the inventor mutated the existing antibody against VZV envelope protein gE to obtain a mutated antibody, and unexpectedly found that the mutated antibody is especially suitable for the detection of VZV virus titer , especially when used for fluorescence detection, it can achieve comparable accuracy to the traditional plaque method, but its detection time is significantly shortened

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  • Method for rapidly detecting varicella virus titer by using fluorescence method
  • Method for rapidly detecting varicella virus titer by using fluorescence method
  • Method for rapidly detecting varicella virus titer by using fluorescence method

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Embodiment Construction

[0047] The following embodiments further illustrate the content of the present invention, but should not be construed as limiting the present invention. Without departing from the spirit and essence of the present invention, the modifications or replacements made to the methods, steps and conditions of the present invention all belong to the present invention range.

[0048] The most critical technology for detecting varicella-zoster virus titer by fluorescence method is the selection of virus dilution factor and fluorescent staining. Only by selecting the appropriate virus dilution range and the correct fluorescent staining method can an accurate virus titer be obtained.

[0049] 1. Test material

[0050] (1) Cell matrix: diploid cells, such as MRC-5 cells, 2BS cells, KMB-17 cells, etc.;

[0051] (2) Cell culture medium: MEM + 10% newborn bovine serum + 1% glutamine + 2% NaHCO 3 ;

[0052] (3) Virus diluent: MEM + 2% newborn bovine serum + 1% glutamine + 2% NaHCO 3 , or P...

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Abstract

The invention provides an antibody for detecting varicella-zoster virus (VZV) titer and a related detection method. The method comprises the following steps: inoculating diploid cells into a 96-well plate, culturing, infecting a sample inoculated with unknown virus titer, culturing, adding an antibody specifically combined with VZV virus and an FITC labeled secondary antibody, carrying out fluorescence staining, and observing the infected lesion number under a microscope to obtain the virus titer of the sample. The method provided by the invention is strong in specificity, sensitive, rapid andeasy to observe, can obtain the same detection accuracy as a traditional speckle method, and can significantly shorten the detection time.

Description

Technical field: [0001] The invention belongs to the technical field of biological detection, and in particular relates to the titer detection of varicella-zoster virus and a high-throughput and rapid detection method for varicella-zoster virus titer established by a fluorescent immunological method. Background technique: [0002] Varicella-zoster virus (VZV), which belongs to the genus Human Herpesvirus, is a human herpesvirus type 3. It was first isolated from the skin herpes fluid of children with chickenpox by Japanese scholar Takahashi M in 1971. Follow-up studies have shown that VZV belongs to the subfamily of herpesvirus α, is a double-stranded DNA virus, consists of 124.9kb, has a diameter of 150-200nm, contains 71 genomes, and encodes 67 proteins, but the virus itself has only one serotype and has neurotropism. sex. VZV has no animal storage host, and humans are the only natural host. The skin is the main target organ of the virus. The initial infection of human...

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Application Information

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IPC IPC(8): G01N33/577G01N33/569C07K16/08
CPCC07K16/088G01N33/56994G01N33/577G01N2333/04
Inventor 李春明赵海波梁慧颖闫磊王玮朱晓文周慧沈红杰
Owner CHANGCHUN KEYGEN BIOLOGICAL PROD
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