A strain of Paecilomyces lilacinus and its application in inhibiting plant growth
A technology of Paecilomyces lilacinus and Paecilomyces lilacinus, which is applied in the field of microorganisms, can solve the problems of rising populations of drug-resistant weeds, river pollution, excessive pesticide residues in soil, etc., so as to prolong the germination time, have obvious effects and inhibit the obvious effect
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Embodiment 1
[0055] The screening, separation and identification of embodiment 1 Paecilomyces lilacinus
[0056] 1. Isolation and purification of bacterial strains
[0057] Wash the stems and roots of purslane (from a certain place in Guangzhou, Guangdong) with tap water, dry them in the air, soak them in 75% (w / w) alcohol for 2 minutes, rinse them with sterile water, and rinse them with 0.1% (w / w) mercuric chloride Soak for 1.5min, rinse with sterile water; wash leaves with tap water, dry, soak in 75% (w / w) alcohol for 2min, rinse with sterile water, soak in 0.1% (w / w) mercuric chloride for 30s, rinse with sterile water rinse. The sterile water-coated plate (LB) cleaned for the last time was used as a blank control to exclude microbial interference on the surface of purslane, and the roots, stems and leaves were cut into small pieces and planted on PDA medium, and cultivated in a 28°C incubator. The grown bacteria were continuously purified by the plate line drawing method until they be...
Embodiment 2
[0067] The influence of different solvent extracts of embodiment 2 Paecilomyces lilacinus culture fluid on plant growth
[0068] (1) Prepare 1 L of PDB culture solution (ie, liquid PDB medium), put it into a 2 L Erlenmeyer flask, seal it, and sterilize it under high temperature and high pressure at 121°C for 30 minutes before use. Under sterile conditions, Paecilomyces lilacinus Scaumcx04 (a strain with a diameter of 0.5 cm) was inoculated in shake flasks filled with PDB culture solution, and then the inoculated shake flasks were placed on a shaker for 7 days, and the shaker The temperature is 28°C and the rotation speed is 180r / min. After culturing for one week, the culture solution was taken out to obtain the corresponding endophytic bacteria solution.
[0069] (2) Filter the obtained bacterial liquid with gauze (to remove large truffles) to obtain the supernatant, and divide it into three equal parts, then add an equal volume of n-butanol, ethyl acetate and petroleum in th...
Embodiment 3
[0077] Embodiment 3, the influence of the ethyl acetate extract of Paecilomyces lilacinus culture fluid on the growth of different plants
[0078] (1) Prepare 1L of PDB culture solution, put it into a 2L Erlenmeyer flask, seal it, and sterilize it under high temperature and high pressure at 121°C for 30 minutes before use. Under sterile conditions, Paecilomyces lilacinus Scaumcx04 (a strain with a diameter of 0.5 cm) was inoculated in shake flasks filled with PDB culture solution, and then the inoculated shake flasks were placed on a shaker for 7 days, and the shaker The temperature is 28°C and the rotation speed is 180r / min. After culturing for one week, the culture solution was taken out to obtain the corresponding endophytic bacteria solution.
[0079](2) Filter the obtained bacterial solution with gauze to obtain a supernatant, and then add an equal volume of ethyl acetate to the supernatant for extraction. After extraction, the obtained organic phase was transferred to ...
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