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Tumor timely drug accurate treatment model based on chicken embryo chorionic allantoic sac

A technology of chorionic villi and chicken embryos, applied in the field of tumor timely drug precise treatment model and its establishment, can solve the problem that the observable time is only 8-10 days, and achieve the effect of alleviating pain

Pending Publication Date: 2020-08-28
任宏政
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

In addition, it is cheap, easy to operate, and easy to observe, but the disadvantage is that the observable time is only 8-10 days

Method used

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  • Tumor timely drug accurate treatment model based on chicken embryo chorionic allantoic sac
  • Tumor timely drug accurate treatment model based on chicken embryo chorionic allantoic sac
  • Tumor timely drug accurate treatment model based on chicken embryo chorionic allantoic sac

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specific Embodiment

[0042] 1. Chicken embryo PDX model establishment

[0043] 1.1 Fertilized egg preparation for incubation

[0044] Fertilized eggs should be placed at around 13°C for incubation. The temperature should not be too high or too low. Even if it is around 13°C, the storage time should not exceed 1 week.

[0045] 1.2 Constant temperature incubation of fertilized eggs

[0046] The incubation temperature of the eggs is 37.5°C and the humidity is about 62%.

[0047] 1.3 Embryo incubation;

[0048] The chicken fertilized eggs are prepared for incubation, the fertilized eggs are incubated at a constant temperature, and the embryos are incubated until the eighth day.

[0049] Open the egg shell, put the embryo, egg white and egg yolk completely into a plastic weighing box, and the egg yolk must not be damaged. During the whole process, you can see the beating heart of millet to mung bean size.

[0050] chicken embryo development image 3 As shown, E8-16 days, the optimal time for tumor...

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Abstract

The invention discloses a tumor timely drug accurate treatment model based on the chicken embryo chorionic allantoic sac. The model comprises chicken embryo PDX model establishment and tumor transplantation drug detection and timely drug accurate treatment analysis; the chicken embryo PDX model establishment and tumor transplantation drug detection comprises the following steps: incubation preparation of fertilized eggs, constant-temperature incubation of the fertilized eggs and embryo incubation are performed; tissue / cells with biological activity are planted on the surface of the CAM, and atarget detection drug is added to the surface; and observation and HE, IHC and molecular pathology detection are carried out; the timely drug accurate treatment analysis comprises the following steps:preliminary treatment of a patient operation specimen is performed, and after fresh tissue treatment or tissue library cryopreservation and conventional pathological examination or molecular pathological gene analysis are completed, targeted drugs and conventional chemotherapeutic drug suggestions are proposed; tissue inoculation on the chicken embryo PDX model is carried out to acquire drug simulation treatment suggestions; comprehensive analysis and individualized accurate treatment are carried out; treatment effect monitoring and analysis are perforemd. The CAM PDX model is applied, and the curative effect and side effects of chemotherapeutic drugs for tumor treatment can be monitored in time.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a tumor timely drug precise treatment model based on chicken embryo choriourinary sac and a method for establishing the same. Background technique [0002] In recent years, with the increase of tumor treatment methods, its effect has not been effectively improved, but its side effects have been bothering patients and medical workers. Therefore, how to carry out timely, effective and precise individualized treatment of tumors is the focus of scientists' research. [0003] PDX models are used as experimental models for cancer research and have grown in popularity in recent years. They can be used to study tumor markers and biological pathways, drug efficacy, etc. for the establishment of personalized cancer treatments. At present, immunodeficiency mice are widely used, but its disadvantage is that the tumor is not intuitive and the observation period is long. The primary culture of ...

Claims

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Application Information

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IPC IPC(8): C12N5/073C12N5/09C12Q1/02
CPCC12N5/0604C12N5/0693G01N33/5011G01N33/5088C12N2503/02G01N2500/10
Inventor 任宏政樊琳蕊王金胜张灏
Owner 任宏政
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