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Handheld dairy product risk pollutant detector and detection method

A handheld, pollutant technology, used in instruments, measuring devices, scientific instruments, etc., can solve problems such as false positives and incomplete detection items, and achieve the effects of less sample volume, reduced drift probability, and strong selectivity.

Inactive Publication Date: 2020-08-28
迅检(义乌)科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the biotoxins produced by feed mildew eventually remain in dairy products, the human body faces biosafety risks such as aflatoxin, ochratoxin, and vomitoxin. The current chromatography and mass spectrometry technologies cannot meet the needs of enterprises for self-inspection. ELISA There are still some "false positives" and incomplete testing items in the rapid methods, which lead to the bottleneck problem of enterprises' self-inspection of biological toxins.

Method used

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  • Handheld dairy product risk pollutant detector and detection method
  • Handheld dairy product risk pollutant detector and detection method
  • Handheld dairy product risk pollutant detector and detection method

Examples

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Embodiment 1

[0063] Such as figure 1 As shown, the handheld dairy product risk pollutant detector provided by the present invention includes an optical system, a microfluidic sample pool 3 and a signal processing system.

[0064] The optical system includes an LED excitation light source 1, a convex lens 2, a biconical optical fiber sensor 4, an optical fiber connector 5, a jumper wire 6, an optical filter 7, and a photodiode detector 8.

[0065] The microfluidic sample pool 3 includes a sample inlet and a sample outlet.

[0066] The signal processing system includes a fluorescent signal photoelectric conversion module 9, a weak signal preamplifier 10, a digital signal processing module 11 and a display screen 12;

[0067] The excitation light emitted by the LED excitation light source 1 enters the biconical optical fiber sensor 4 at a certain angle through the convex lens 2. The excitation light propagates in the biconical optical fiber sensor 4 in the form of total internal reflection,...

Embodiment 2

[0071] Utilize the method for the hand-held rapid detection instrument that embodiment 1 provides to detect pollutant aflatoxin, comprising:

[0072] 1) The optical fiber of the bitapered optical fiber sensor in the handheld rapid detection instrument is modified according to the following method for biorecognition molecules:

[0073] After immersing the optical fiber in the piraha solution for 30 minutes, silanizing it with an APTES aqueous solution with a mass percent concentration of 4.5% after cleaning with ultrapure water;

[0074] In the piraha solution, the volume ratio of concentrated sulfuric acid and hydrogen peroxide is 3:1;

[0075] 2) Soak the silanized optical fiber obtained in step 1) in 4% glutaraldehyde aqueous solution, shake and react at room temperature for 1 hour, and obtain a tapered optical fiber with bifunctional groups on the surface;

[0076] Then soak the obtained tapered optical fiber with bifunctional groups on its surface in AFM with a concentrat...

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Abstract

The invention discloses a handheld dairy product risk pollutant detector and a detection method. According to the invention, the advantages of fluorescence detection and biological recognition principles are combined, so that the handheld dairy product risk pollutant detector has the characteristics of high detection sensitivity, high detection speed, simple operation, good selectivity and the like; by adopting the biconical optical fiber sensor, the excitation efficiency and the fluorescence collection efficiency of excitation light can be effectively improved, and the sensitivity of an instrument is improved. The method can be used for high-sensitivity rapid detection of dairy product risk pollutants.

Description

technical field [0001] The invention belongs to the field of food detection, and relates to a hand-held dairy product risk pollutant detector and a detection method. Background technique [0002] Food is the foundation of the people, and safety is the first priority in food. As the process of economic globalization is accelerating, food safety is not only related to the health of consumers, but also affects international trade in food and agricultural products, and has become one of the focal issues of the international community. Because the biotoxins produced by feed mildew eventually remain in dairy products, the human body faces biosafety risks such as aflatoxin, ochratoxin, and vomitoxin. The current chromatography and mass spectrometry technologies cannot meet the needs of enterprises for self-inspection. ELISA There are still some problems of "false positive" and incomplete testing items in rapid methods, which lead to the bottleneck problem of enterprises' self-insp...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N33/533
CPCG01N21/6402G01N21/6428G01N33/533
Inventor 王宏亮
Owner 迅检(义乌)科技有限公司
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