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Protein separation membrane based on interface assembly mode as well as preparation method and application thereof

A protein separation and interface technology, applied in the field of protein separation membranes, can solve the problems of complex preparation process, and achieve the effects of easy large-area preparation, good biodegradability, and low energy consumption

Inactive Publication Date: 2020-09-01
JILIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the defects such as the complex preparation process of polymer membranes in the prior art, the present invention provides a protein separation membrane based on an interface assembly method, a preparation method and the application of the separation membrane. The preparation method is simple and easy to realize large-area preparation At the same time, it has the characteristics of low cost, low energy consumption, and environmental protection, which can avoid the cumbersome steps and environmental pollution problems in the traditional polymer membrane synthesis process

Method used

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  • Protein separation membrane based on interface assembly mode as well as preparation method and application thereof
  • Protein separation membrane based on interface assembly mode as well as preparation method and application thereof
  • Protein separation membrane based on interface assembly mode as well as preparation method and application thereof

Examples

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Effect test

Embodiment 1

[0038] Mix 1ml of 2mg / ml BSA in PBS buffer solution (pH=7) and 1ml of 3mg / ml CTAB aqueous solution in equal volumes in a petri dish with a diameter of 3.5cm, then add 400μL of 2-ethyl-1-hexanol solution, Then add 25 μL of glutaraldehyde solution to the solution, and incubate at room temperature for 1-2 hours, and a layer of BSA protein two-dimensional nano film can be observed at the oil-water interface.

Embodiment 2

[0040] In this embodiment, the PBS buffer solution (pH=7) and 3 mg / ml BSA in Example 1 were replaced with an equal volume of 4 mg / mL BSA in PBS buffer solution (pH=7) and 1 ml of 6 mg / ml CTAB aqueous solution. CTAB aqueous solution, the other steps are the same as in Example 1 to obtain a separation membrane.

Embodiment 3

[0042] In this embodiment, replace the PBS buffer solution (pH=7) of 2 mg / ml BSA and the 3 mg / ml BSA in Example 1 with an equal volume of 6 mg / mL BSA in PBS buffer solution (pH=7) and 1 ml of 9 mg / ml CTAB aqueous solution. CTAB aqueous solution, the other steps are the same as in Example 1 to obtain a separation membrane.

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Abstract

The invention discloses a protein separation membrane based on an interface assembly mode as well as a preparation method and application thereof, and belongs to the technical field of protein separation membranes. The separation membrane is a two-dimensional nano-film formed by self-assembly after bovine serum albumin (BSA) and a surfactant are subjected to electrostatic interaction on an oil-water interface. The separation membrane provided by the invention has the advantages of low cost, environmental friendliness, simple preparation process and the like, and has good biocompatibility. According to the characteristics of interface assembly, the size of the separation membrane is determined by the size of the interface, so that large-area preparation is easier to realize. The separationmembrane has a selective separation effect on molecules with different charges, can completely intercept molecules with negative charges, has a high diffusion rate on small molecules with positive charges, and is an ideal separation membrane.

Description

technical field [0001] The invention belongs to the technical field of protein separation membranes, and in particular relates to a protein separation membrane based on an interface assembly method, a preparation method and an application thereof. Background technique [0002] In recent years, membrane separation technology has developed rapidly and has been listed as a key development project by many countries in the world. [0003] As a high-tech, membrane separation has been widely used in environmental protection, water treatment, chemical industry, metallurgy, energy, food and other fields. In the research field of membrane separation, the research on membrane materials is an important content. At present, the commonly used membrane materials are mainly organic synthetic polymer materials. However, the preparation methods of polymer membranes are complicated, which brings inevitable limitations in the preparation process and affects the separation performance of biomem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D69/02B01D71/06B01D67/00C09B57/00C09B29/08C09B21/00C09B67/54
CPCB01D67/0002B01D67/0006B01D69/02B01D71/06C09B21/00C09B29/0803C09B57/00C09B67/0096
Inventor 李茹玉刘俊秋
Owner JILIN UNIV
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