Detection method for judging intestinal alkaline phosphatase activity level in intestines and related application

A technology of phosphatase activity and detection method, which is applied in the detection field of judging the intestinal alkaline phosphatase activity level in the intestinal tract, and can solve the problems of unfavorable promotion, high cost of detection reagents and detection methods, etc.

Pending Publication Date: 2020-09-04
李鑫荣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method for detecting alkaline phosphatase is to detect by feces sampling, and the cost of detection reagents and detection methods is relatively high, which is not conducive to popularization

Method used

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  • Detection method for judging intestinal alkaline phosphatase activity level in intestines and related application
  • Detection method for judging intestinal alkaline phosphatase activity level in intestines and related application
  • Detection method for judging intestinal alkaline phosphatase activity level in intestines and related application

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Effect test

Embodiment 1

[0071] Objective: To study metabolic surgery in patients with type 2 diabetes and obesity

[0072] Methods: This study investigated 104 severely obese patients and 66 obese patients with type 2 diabetes treated in Changchun Jiahe Surgical Hospital from 2015 to 2019. There were 21 males and 83 females with severe obesity, aged 15-50 years, with an average age of 30.61 years. There were 33 males and 33 obese patients with type 2 diabetes, aged 17-68 years, with an average age of 41.91 years. The changes of urine pH, blood sugar, blood lipid and body weight of patients before and after metabolic surgery were detected by clinical laboratory. Note: The clinical research was approved by the Ethics Committee of Changchun Jiahe Surgical Hospital.

[0073] result:

[0074] Metabolic surgery significantly reduced blood sugar and blood lipids in patients with type 2 diabetes and obesity, and significantly increased urine pH levels in patients with diabetes and obesity (Table 1, 2, 3, ...

Embodiment 2

[0096] The inventors of the present invention hypothesize that the acidic urine pH (4.5-5.5) of patients with metabolic syndrome (hypertension, hyperlipidemia, and hyperglycemia) may be caused by excessive acid load in the gastrointestinal tract. and high-protein foods. In order to further investigate whether there is a relationship between urine and intestinal pH levels, the following test method was performed:

[0097] 1. Comparative study on the pH value of urine after eating acid and alkali between healthy and unhealthy people

[0098] A total of 30 test participants were divided into 2 groups according to whether the urine pH was > 6, namely the healthy group and the non-healthy group. Participants in the two groups took 500ml of Sprite, 8 tablets of sodium bicarbonate (0.5g / tablet), 180g of braised pork (cooked), 180g of high-temperature, low-oil, and low-oil, respectively, from the first day of the test to the fourth day of the test at dinner time and before going to b...

Embodiment 3

[0109] Purpose: Determination of alkaline phosphatase activity in human plasma, small intestine and stool

[0110] Methods: The whole blood of the test participants was drawn into non-pyrogenic blood collection tubes containing heparin sodium, centrifuged at 4°C (3500g / min, 8min), and the plasma was collected; 1.00g of crushed small intestine tissue was taken, and Tris-Hcl buffer (50mmol / L, pH8.0) 10mL; take 1.00g of test participant's feces, add Tris-Hcl buffer (50mmol / L, pH8.0) 10mL, shake and mix well, centrifuge at 4℃ (12000g / min, 10min), The supernatant was taken, diluted, and its activity was measured at a wavelength of 510 nm using an alkaline phosphatase kit (Leagene Biological Ltd, catalog #TE0005).

[0111] result:

[0112] The human small intestine contains high concentrations of alkaline phosphatase, which is released in large quantities into the feces and accumulates to even higher concentrations of alkaline phosphatase (Table 10).

[0113] Table 10. Alkaline Pho...

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Abstract

The invention discloses a detection method for judging the intestinal alkaline phosphatase activity level in intestines and a related application and belongs to the field of detection. The gastrointestinal acid-alkaline load level and the pH-related intestinal alkaline phosphatase activity level in intestines are indirectly judged through pH detection of urine, the gastrointestinal acid-alkaline load is positively correlated with the pH of the urine, and the intestinal alkaline load is positively correlated with the intestinal alkaline phosphatase activity level; and the edible dose and curative effect of a taken intestinal alkalization preparation in treatment of intestinal alkaline phosphatase activity-related diseases can be judged. The study also shows that the dose when the pH of theurine is 6.4 or above after treatment through taking the intestinal alkalization preparation (for example, intestinal alkalization and fat absorption blocking mineral explosion particles containing microcapsule coated sodium butyrate) is beneficial to treatment of the intestinal alkaline phosphatase activity-related diseases (hyperglycemia, hyperlipidemia and colitis). The method is fast, simple and effective through the pH detection of the urine.

Description

technical field [0001] The invention relates to the field of detection, in particular to a detection method for judging the activity level of intestinal alkaline phosphatase in the intestinal tract and related applications. Background technique [0002] Type 2 diabetes is a chronic metabolic disease that occurs when tissues do not produce enough insulin and cannot use insulin effectively (insulin resistance). Tissue insulin resistance and insufficient insulin production leading to chronically elevated blood glucose levels with adverse effects on the eyes, heart, kidneys and feet are known as complications of diabetes (1). Type 2 diabetes is often accompanied by hyperlipidemia, hypertension and obesity, which is called metabolic syndrome. Thus, type 2 diabetes is generally part of the metabolic syndrome (2). [0003] In recent years, large-scale human clinical studies have shown that the urine pH of patients with metabolic syndrome (hypertension, hyperlipidemia, and hypergl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/42G01N33/493
CPCC12Q1/42G01N33/493
Inventor 高辰哲李傲辰徐庆李鑫荣韩建春惠觅宙
Owner 李鑫荣
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