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Peripheral blood TCR marker for endometrial cancer and detection kit and application thereof

An endometrial cancer and marker technology, applied in the field of genetic engineering, can solve the problems of CA125 elevation, unpredictable recurrence, elevation, etc., and achieve the effects of simple sampling, low labor cost, high specificity and accuracy

Pending Publication Date: 2020-09-04
CHENGDU EXAB BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is worth noting that in patients with peritoneal inflammation or radiation injury, CA125 may be abnormally elevated
In contrast, patients with isolated vaginal metastases do not have elevated CA125 levels, so recurrence cannot be predicted in the absence of other clinical findings

Method used

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  • Peripheral blood TCR marker for endometrial cancer and detection kit and application thereof
  • Peripheral blood TCR marker for endometrial cancer and detection kit and application thereof
  • Peripheral blood TCR marker for endometrial cancer and detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1: Obtaining endometrial cancer TCR marker CDR3 sequence set by immunographic analysis

[0049] 1. Sampling and immune map analysis

[0050] Collect 1301 control groups (including healthy people and patients with non-tumor diseases, 1300 people for model building, 1 healthy person for verification), 21 endometrial cancer patients (20 people for model building, 1 person for Verification) and peripheral blood (10mL per person) of a subject with unknown health status, the amino acid sequence of the epitope 3 (CDR3) of the TCR of the subject and the control group was obtained by high-throughput sequencing, ensuring the identity of each sample The total number of CDR3 sequences of functional TCRs should not be less than 30,000;

[0051] 2. The CDR3 sequence of the TCR of each sample is randomly sampled without replacement, so that the total number of CDR3 sequences of each sample is 30,000. For any specific CDR3 sequence X, the number of repeated occurrences in the...

Embodiment 2

[0057] Example 2: Verification of the specificity of the endometrial cancer TCR marker CDR3 sequence set

[0058] 1. Sampling and immune map analysis

[0059] Peripheral blood (10mL per person) was collected from 319 tumor patients without endometrial cancer and 6 subjects with unknown health status, and the epitope 3 (CDR3 ) amino acid sequence to ensure that the total number of CDR3 sequences of functional TCRs in each sample is not less than 30,000; random non-replacement sampling is performed on the CDR3 sequences of TCRs in each sample, so that the total number of CDR3 sequences in each sample is 30,000 .

[0060] 1. Randomly select 100 healthy people and 45 non-tumor disease patients from the control group in Example 1.

[0061] 3. According to 100 healthy people, 45 non-tumor disease patients, and 25 endometrial cancer patients from Example 1, and 319 non-endometrial cancer tumor patients and 6 unknown health conditions newly acquired in Example 2 The subject's immun...

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Abstract

The invention discloses a peripheral blood TCR marker of endometrial cancer as well as a detection kit and application thereof. The marker comprises at least one of proteins as shown in sequences SEQID NO.1-100. The invention also discloses a kit for detecting the peripheral blood TCR marker of endometrial cancer. The method is based on a high-throughput sequencing method, and includes steps of:drawing only a small amount of peripheral blood and extracting RNA, treating a sample to establish an immune map library, carrying out high-throughput sequencing and TCR data analysis, determining acharacteristic TCR sequence in peripheral blood of the endometrial cancer, and then comparing a test result of the sample to be tested with the characteristic TCR sequence so as to determine whether the endometrial cancer exists or not. According to the invention, a huge number of endometrial cancer specific TCR sequences can be compared at the same time, and compared with single detection of oneor more markers, the peripheral blood TCR marker has higher specificity and accuracy, and the diagnosis efficiency is improved.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a peripheral blood TCR marker of endometrial cancer, a detection kit and application thereof. Background technique [0002] Endometrial cancer is a group of epithelial malignant tumors that occur in the endometrium, and it is more common in perimenopausal and postmenopausal women. It is one of the most common female reproductive system tumors, with nearly 200,000 new cases each year, 75% to 95% of which recur within 2 to 3 years after surgery, and it is the third common gynecological malignancy that causes death. The cause of the disease is not very clear. The key to improving the prognosis lies in early detection, early diagnosis and early treatment. Currently, there is no screening method, and only clinical diagnosis under the guidance of a gynecologist is performed when clinical symptoms such as vaginal bleeding, vaginal discharge, and lower abdominal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/574A61K39/00A61P35/00
CPCG01N33/57442G01N33/57488A61K39/001111A61P35/00G01N2333/7051
Inventor 张志新杨鑫卓越钟雪梅杨诗涵高浩
Owner CHENGDU EXAB BIOTECH CO LTD
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