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A natural ingredient that inhibits the synthesis of melanin and its application in cosmetics

A technology of natural ingredients and uses, applied in the direction of cosmetics, cosmetic preparations, medical preparations containing active ingredients, etc., can solve the problems of research on the activity and whitening of polysaccharide F1 without purification

Active Publication Date: 2020-12-18
广东梵蜜琳生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Shi Ruiqin et al. isolated three highly homogeneous purified polysaccharides F1, F2, and F3 from Chlorella (Separation, Purification and Physicochemical Properties of Chlorella Polysaccharides, Food Science, September 2019). At present, there is no purified polysaccharide F1 that inhibits melanin. Research on synthesis, inhibition of tyrosinase activity and whitening

Method used

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  • A natural ingredient that inhibits the synthesis of melanin and its application in cosmetics
  • A natural ingredient that inhibits the synthesis of melanin and its application in cosmetics
  • A natural ingredient that inhibits the synthesis of melanin and its application in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1: Enzyme test

[0027] 1. Test materials

[0028] Purified polysaccharide F1 was prepared according to the above-mentioned literature method and stored at -20°C.

[0029] The purity of positive control β-arbutin is not less than 98%.

[0030] The water used in the experiment was Wahaha pure water, and the chemical reagents were all conventional reagents.

[0031] 2. Test method

[0032] Reference method (synthesis of cinnamic acid-coumarin ester analogs and study on their inhibition of tyrosinase activity, Organic Chemistry, Chin.J.Org.Chem.2020,40) Determination of purified polysaccharide F1 and positive control against tyrosine Enzyme inhibition rate:

[0033] The purified polysaccharide F1 and the positive control were respectively dissolved in 50% DMSO aqueous solution and diluted into solutions with different concentrations. After mixing 130 μL phosphate buffer solution (50 mM, pH=6.8), 10 μL tyrosinase solution (final concentration 33.3 U / mL) and 10 ...

Embodiment 2

[0039] Embodiment 2: cell test

[0040] 1. Test materials

[0041] Purified polysaccharide F1 was prepared according to the above-mentioned literature method and stored at -20°C.

[0042] Mouse melanoma B16-F10 cells were purchased from ATCC.

[0043] DMEM high glucose medium and FBS were purchased from Gibco.

[0044] 2. Test method

[0045] 1. Cell culture

[0046] Mouse melanoma B16F10 cells were incubated with DMEM high glucose medium containing 10% FBS and 1% double antibody at 37°C, 5% CO 2 , cultured and passaged under saturated humidity conditions.

[0047] 2. Determination of inhibition of melanin content

[0048]Reference method (Structure modification of curcumol and inhibition of melanin content research, natural product research and development, Nat Prod Res Dev 2020, 32), using NaOH lysis method to determine the content of melanin in cells:

[0049] B16F10 cells in the logarithmic growth phase were taken, digested to make a cell suspension, and seeded in a...

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Abstract

The invention discloses a natural component for inhibiting melanin synthesis and application of the natural component in cosmetics. It is found in the invention that purified polysaccharide F1 separated from chlorella disclosed in the prior art has tyrosinase inhibitory activity; and synthesis of melanin can be inhibited by inhibiting the expression of melanin synthesis key proteins MITF and TYR,and finally, the content of melanin in cells is reduced. Therefore, the purified polysaccharide F1 has whitening activity and has the prospect of being developed into whitening cosmetics (including but not limited to facial cleansers, face creams, hand creams, emulsions, masks and the like).

Description

technical field [0001] The invention belongs to the field of cosmetics and relates to a natural ingredient for inhibiting melanin synthesis and its application in cosmetics. Background technique [0002] Melanin is a biopigment synthesized and secreted by the cells itself, which is insoluble in water and soluble in alkaline solutions. It belongs to protein derivatives and can absorb ultraviolet rays to protect the skin from various damages caused by ultraviolet rays, including photoaging and inflammation. and cancer etc. However, excessive synthesis or abnormal synthesis of melanin, such as freckles and vitiligo, will have a certain impact on the face, and even bring psychological and mental stress to the patient, seriously affecting the patient's living conditions. Melanin is synthesized by melanocytes located in the basal cell layer of the epidermis developed from neural crest cells, and then transported to keratinocytes through dendritic structures where it is rearranged...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K8/73A61Q19/02
CPCA61K8/73A61K2800/782A61K2800/805A61K2800/84A61Q19/02C08B37/0003
Inventor 王伟陈鹏其他发明人请求不公开姓名
Owner 广东梵蜜琳生物科技有限公司