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Peripheral blood TCR marker for prostate cancer, and detection kit and application thereof

A technology of prostate cancer and markers, applied in the field of genetic engineering, can solve the problems of over-diagnosis of prostate cancer, missed opportunities for canceration, over-treatment, etc., and achieve the effects of lower detection costs, simple sampling, high specificity and accuracy

Pending Publication Date: 2020-09-18
CHENGDU EXAB BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, because prostate cancer has no obvious symptoms in the early stage, or the symptoms are similar to those of benign prostatic hyperplasia and prostatitis, many patients will miss the opportunity of early detection of cancer due to reasons such as resistance to digital rectal examination
On the other hand, according to research in the United States, the use of PSA and other methods to screen for prostate cancer has the problems of overdiagnosis and overtreatment

Method used

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  • Peripheral blood TCR marker for prostate cancer, and detection kit and application thereof
  • Peripheral blood TCR marker for prostate cancer, and detection kit and application thereof
  • Peripheral blood TCR marker for prostate cancer, and detection kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 Obtaining Prostate Cancer TCR Marker CDR3 Sequence Set by Immunogram Analysis

[0044] 1. Sampling and immune map analysis (method reference patent ZL201910300069.9)

[0045] Collect 1301 control groups (including healthy people and patients with non-tumor diseases, 1300 people for model building, 1 healthy person for verification), 11 prostate cancer patients (10 people for model building, 1 person for verification) and the peripheral blood of a subject with unknown health status (10mL per person), and the amino acid sequence of the TCR epitope 3 (CDR3) of the subject and the control group was obtained by high-throughput sequencing to ensure the functionality of each sample The total number of CDR3 sequences of TCR should not be less than 30,000;

[0046] 2. The CDR3 sequences of the TCR of each sample are randomly sampled without replacement, so that the total number of CDR3 sequences of each sample is 30,000. For any specific CDR3 sequence X, the number o...

Embodiment 2

[0052] Example 2 Verification of the specificity of the prostate cancer TCR marker CDR3 sequence set

[0053] 1. Sampling and immune map analysis (method reference patent ZL201910300069.9)

[0054] Peripheral blood (10 mL per person) was collected from 339 non-prostate cancer tumor patients and 2 subjects with unknown health status, and the epitope 3 (CDR3) amino acid of TCR in the subjects and the control group was obtained by high-throughput sequencing Sequences, to ensure that the total number of CDR3 sequences of functional TCRs in each sample is not less than 30,000; random non-replacement sampling is performed on the CDR3 sequences of TCRs in each sample, so that the total number of CDR3 sequences in each sample is 30,000.

[0055] 2. Randomly select 100 healthy people and 45 non-tumor disease patients from the control group in Example 1.

[0056] 3. According to 100 healthy people, 45 non-tumor disease patients, 10 prostate cancer patients from embodiment 1, and 339 no...

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Abstract

The invention discloses a peripheral blood TCR marker for prostate cancer, and a detection kit and application of the peripheral blood TCR marker. The peripheral blood TCR marker comprises at least one of proteins of which the sequences are shown as SEQ ID NO. 1-100. The peripheral blood TCR marker, the detection kit and the application thereof are based on a high-throughput sequencing method, only a small amount of peripheral blood needs to be collected for extracting RNA, an immune map library is established by treating samples, a characteristic TCR sequence in prostate cancer peripheral blood is firstly determined through high-throughput sequencing and TCR data analysis, and then a test result of a sample to be tested is compared with the characteristic TCR sequence so as to determine whether a patient has prostate cancer or not. The peripheral blood TCR marker can simultaneously compare a huge number of prostate cancer specific TCR sequences, has higher specificity and accuracy compared with single detection of one or more markers, and improves the diagnosis efficiency.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a peripheral blood TCR marker of prostate cancer, a detection kit and application thereof. Background technique [0002] Prostate cancer refers to epithelial malignant tumors that occur in the prostate. According to the 2004 WHO "Pathology and Genetics of Tumors of the Urinary System and Male Genital Organs", the pathological types of prostate cancer include adenocarcinoma (acinar adenocarcinoma), ductal adenocarcinoma, urothelial carcinoma, squamous cell carcinoma, and adenosquamous carcinoma. Cancer, of which prostate adenocarcinoma accounts for more than 95%, therefore, what we usually call prostate cancer refers to prostate adenocarcinoma. [0003] The main risk factors for prostate cancer include heredity and family factors, and are also related to living habits. It has been reported that the incidence of prostate cancer is related to sexual activit...

Claims

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Application Information

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IPC IPC(8): G01N33/574A61K39/00A61P35/00
CPCG01N33/57434G01N33/57488A61K39/001111A61P35/00G01N2333/7051A61K2039/884
Inventor 张志新杨鑫卓越文学平治想林
Owner CHENGDU EXAB BIOTECH CO LTD
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