Inducer for promoting vegetative propagation of plants and application thereof

Abstract

The invention relates to the technical field of biology, and in particular, relates to an inducer for promoting vegetative propagation of plants and an application thereof. The inducer comprises 1000parts of arginine, 20 parts of naphthylacetic acid, 20 parts of 6-benzyladenine, 2 parts of epibrassinolide, 100-300 parts of sodium nitroprusside, and 20 parts of jasmonic acid methyl ester or 20 parts of salicylic acid. A germination accelerating agent comprises 50 parts of gibberellin 4+7, 10 parts of indoleacetic acid and 20-40 parts of fluazinone. According to the inducer disclosed by the invention, the number of sub-bulbs generated by induction on a mother bulb can be remarkably increased; the germination rate of the sub-bulbs is remarkably improved by the germination accelerating agent;artificial induction asexual clone propagation is successfully achieved by the inducer, and compared with a traditional seedling raising method, the time for producing small seed stems for field transplanting is shortened by one year, the cultivation time is shortened, and the investment of manpower and material resources for planting is reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an inducer for promoting asexual reproduction of plants and its application. Background technique [0002] Gansu Fritillaria (F.przewalshii Maxim) is one of the medicinal plants of the precious medicinal material Chuan Fritillaria, mainly distributed in the alpine shrubs and grasses above 2800 meters above sea level in Minxian, Wudu, Zhouqu, Yuzhong and other places in Gansu Province , Cool and shade is the main feature of the climate in its distribution area. Gansu fritillary grows slowly, has harsh environmental requirements, low reproduction coefficient, and high incidence of root rot, which has severely restricted the development of artificial planting industry. It is urgent to solve the reproduction problem of Fritillaria fritillaria in Gansu. Under natural conditions, Fritillaria can only be propagated by seeds, and the seeds are morphologically and physiologically mature. It...

AI Technical Summary

Problems solved by technology

Under natural conditions, Fritillaria can only be propagated by seeds, and the seeds are morphologically and physiologically mature. It takes 2 years to breed small stems through seedlings, and root rot, blight and other seedling diseases are serious, which affect Fritillaria seedlings At the same time, Fritillaria seedlings are susceptible to the impact of low temperature in early spring and cold in late spring, the seedling rate is low, and farming operations are time-consuming, labor-intensive and labor-intensive, resulting in the planting of Fritillaria cannot meet market demand

[0003] The patented Gansu Fritillaria test-tube bulblet cultivation method (CN201510393001.1) discloses a technique for rapidly producing test-tube bulblets through tissue culture, which effectively solves the problems of low protocorm induction rate, long test-tube bulblet culture cycle, and cumbersome steps problem, but the production cost is high, and it requires specialized technicians to operate, which requires high skill of the laborers; the invention patent of a bulb flap propagation method of Gansu Fritillaria (CN201410400906.2) discloses a bulb split, gradually changing temperature The storage method has the advantages of simple operation and effective shortening of the cultivation period of Fritillaria fritillaria, but the reproduction coefficient is low

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