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Pinctada martensi Kunitz-type serine protease inhibitor gene, coded protein and application

A technology of serine protease and Pinctada martensii, applied in the field of genetic engineering, can solve the problems such as few reports of antimicrobial peptides and no reports of antimicrobial peptide sequences of Pinctada martensii

Active Publication Date: 2020-09-22
GUANGDONG OCEAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Antimicrobial peptides are the most important link in the biological immune mechanism of shellfish, and are also the most important line of defense for shellfish against pathogen invasion. Understanding the composition and characteristics of various antimicrobial peptide molecules in pinnacle oysters is crucial for developing the source of antimicrobial peptides in pinnacle oysters. New biological antibiotics are of great significance, but there are few reports on antimicrobial peptides from Pinctada martensii, and there is no antimicrobial peptide sequence report in the database

Method used

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  • Pinctada martensi Kunitz-type serine protease inhibitor gene, coded protein and application
  • Pinctada martensi Kunitz-type serine protease inhibitor gene, coded protein and application
  • Pinctada martensi Kunitz-type serine protease inhibitor gene, coded protein and application

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Embodiment 1

[0035] 1 Molecular screening of immune effectors in Pinctada martensii

[0036] From the online antimicrobial peptide database (APD3, http: / / aps.unmc.edu / AP / main.php), PubMed (https: / / www.ncbi.nlm.nih.gov / pubmed / ) and the National Center for Biotechnology Information (NCBI) database (https: / / www.ncbi.nlm.nih.gov / ) to obtain all amino acid sequences with previously validated antimicrobial activity, subsequently, a local reference AMP database was constructed, based on the Pinctada martensii genome Data comparison search. The gene sequence with the highest comparison rate was collected, and then analyzed by using the online Blast in the NCBI database to predict the type of the collected immune effector molecule PmKuPI gene.

[0037] 2 PmKuPI gene-related primer design

[0038] The specific primers of PmKuPI gene were designed using Primer Premier5.0. The 5' end and 3' end specific primers are designed according to the principle of RACE amplification, and the full-length cDNA ...

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Abstract

The invention provides a pinctada martensi Kunitz-type serine protease inhibitor gene, coded protein and application, and belongs to the technical field of application of protein. A nucleotide sequence of the pinctada martensi Kunitz-type serine protease inhibitor gene is shown in SEQ ID No.1 as shown in the description, and an animo acid sequence of the coded protein is shown in SEQ ID No.2 as shown in the description. The provided protein coded by the gene has inhibiting effects on escherichia coli, pseudomonas aeruginosa, aeromonas hydrophila, vibrio parahemolyticus and vibrio harveyi.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a Kunitz-type serine protease inhibitor gene of Pinctada martensii, encoded protein and application. Background technique [0002] In recent years, due to reasons such as high-density farming, eutrophication of aquaculture waters, and deterioration of the ecological environment, various infectious diseases caused by bacteria or viruses have frequently occurred in shellfish cultured species. In the prevention and control process, a large number of traditional drugs such as antibiotics are used, which destroys the micro-ecological balance of the water environment, makes some pathogens resistant to the drugs, and cannot effectively control the occurrence of the disease, resulting in a large number of diseases of Pinctada martensii Death caused huge economic losses to my country's pearl industry. Therefore, understanding the immune mechanism of Pinctada marten...

Claims

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Application Information

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IPC IPC(8): C07K14/81C12N15/15A61K38/57A61P31/04
CPCC07K14/8114A61P31/04A61K38/00Y02A50/30
Inventor 梁海鹰何军军申铖皓房晓宸卢金昭
Owner GUANGDONG OCEAN UNIVERSITY
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