Non-labeled electrochemical immunosensor for detecting prostate specific antigen, and preparation method of non-labeled electrochemical immunosensor
A prostate-specific immunosensor technology, which is applied in the field of non-labeled electrochemical immunosensors for prostate-specific antigen detection and their preparation, can solve the problems of reducing the specific surface area of graphene aerogels, agglomeration of graphene sheets, etc., and achieves excellent results. Biocompatibility, excellent electrical conductivity, the effect of increasing the loading capacity
Active Publication Date: 2020-09-29
JIANGXI SCI & TECH NORMAL UNIV
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However, during the preparation of graphene aerogels, some graphene sheets tend to agglomerate, which reduces the specific surface area of graphene aerogels
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[0033] An electrochemical immunosensor for non-labeled detection of prostate specific antigen, comprising a working electrode, a reference electrode and a counter electrode, wherein the working electrode is made of a composite material of graphene airgel-molybdenum disulfide / gold nanoparticles As a modification material, a prostate-specific antibody serves as a molecular recognition element. The working electrode is a composite material of graphene airgel-molybdenum disulfide / gold nanoparticle, prostate-specific antibody, and bovine serum albumin, which are sequentially modified on the surface of a glassy carbon electrode. The reference electrode is a saturated calomel electrode, and the counter electrode is a platinum sheet electrode. Such as figure 1 Shown is the flow chart of the preparation principle of the working electrode of the electrochemical immunosensor.
[0034] The preparation method of a non-labeled electrochemical immunosensor for detecting prostate specific a...
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The invention discloses a non-labeled electrochemical immunosensor for detecting a prostate specific antigen, and a preparation method thereof, and belongs to the technical field of electrochemical sensors. The non-labeled electrochemical immunosensor comprises a working electrode, a reference electrode and a counter electrode, wherein the working electrode is a glassy carbon electrode, the surface of the glassy carbon electrode is sequentially modified with a graphene aerogel-molybdenum disulfide / gold nanoparticle composite material, a prostate specific antibody and bovine serum albumin, thereference electrode is a saturated calomel electrode, the counter electrode is a platinum sheet electrode, and the prostate specific antibody is used as a molecular recognition element. According to the invention, the constructed non-labeled prostate specific antigen immunosensor has the advantages of wide detection range, low detection lower limit, high sensitivity, simplicity in operation, highdetection speed and the like, the linear detection range is 0.00001 ng / mL-50 ng / mL, and the lowest detection lower limit is 3 fg / mL.
Description
technical field [0001] The invention relates to the technical field of electrochemical sensors, in particular to a non-labeled electrochemical immunosensor for detecting prostate specific antigen and a preparation method thereof. Background technique [0002] Prostate cancer is one of the most common malignancies in men worldwide. Prostate-specific antigen (PSA) is considered as the main biomarker in the diagnosis and prognosis of early prostate cancer. There are many methods for analyzing PSA. Compared with other methods, electrochemical immunosensor has the advantages of rapid detection, high sensitivity and low cost. For electrochemical immunosensors, the characteristics of the materials modified on the electrode surface will affect the detection performance of the sensor. Therefore, to prepare an electrode modification material with excellent performance, it is crucial to amplify the detection signal. [0003] Graphene airgel is a new type of porous carbon material wi...
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IPC IPC(8): G01N27/327G01N27/30G01N27/48G01N33/574G01N33/543
CPCG01N27/3277G01N27/3278G01N27/308G01N27/48G01N33/57434G01N33/57484G01N33/5438
Inventor 段学民胥荃卢丽敏徐景坤贾海燕陈淑贤蒋丰兴
Owner JIANGXI SCI & TECH NORMAL UNIV