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Special micro-ecological feed for stichopus japonicus and preparation method thereof

A production method and micro-ecological technology, applied in the direction of animal feed, animal feed, and animal feed molding or processing, can solve the problems of unstable intestinal flora structure of Apostichopus japonicus, and achieve an all-round adjustment of the environment and absorption mechanism, Improved disease resistance, beneficial addition and survival effects

Inactive Publication Date: 2020-10-02
山东省海洋资源与环境研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to solve the problem that commercial probiotics cannot be colonized in the intestinal tract of sea cucumbers, which leads to the instability of the intestinal flora structure of sea cucumbers, and to provide a compound probiotic combination and application technology that can be used in sea cucumber feed. After the ginseng is eaten, these bacteria can stably colonize the intestinal tract of sea cucumbers, thereby playing the role of fixing the intestinal flora structure, improving the utilization efficiency of nutrients, and promoting the growth of sea cucumbers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] The micro-ecological type sea cucumber special feed of this embodiment is made by mixing the culture medium fermented by probiotics and the sea cucumber wet material, and the parts by weight of each component in the fermented culture medium are respectively: 6 parts of triple probiotics (wherein Bacillus subtilis: Enterococcus faecium: Streptococcus thermophilus (2:2:1), 60 parts of bran, 7 parts of starch, 20 parts of soybean enzymatic hydrolyzate, mix the above into a base material, 4 parts of chitosan oligosaccharide, glucose 3 parts, the total of the two is mixed with water at a weight ratio of 3%, sprayed on the bottom material after mixing, and cultured and fermented in an incubator at 37°C for 14 hours, and then mixed with sea cucumber according to the weight ratio of 4% of sea cucumber wet material. Made by mixing wet ingredients.

[0042] The preparation method of the soybean enzymatic hydrolyzate is as follows: take trypsin and flavor enzyme respectively, and ...

Embodiment 2

[0054] The micro-ecological sea cucumber special feed of this embodiment is made by mixing the culture medium fermented by probiotics and sea cucumber wet material, and the parts by weight of each component in the fermented culture medium are: 4 parts of triple probiotics (wherein Bacillus subtilis: Enterococcus faecium: Streptococcus thermophilus (2:1.5:2), 62 parts of bran, 9 parts of starch, 21 parts of soybean hydrolyzate, mixed well as the base material. 2 parts of chitosan oligosaccharide and 2 parts of glucose, mix the glucose and chitosan oligosaccharide with water at a weight ratio of 4%, and then spray it on the bottom material, and after mixing again, ferment at 38°C for 13 hours, and then follow the 6 times of sea cucumber wet material. % by weight is mixed with sea cucumber wet material.

[0055] The production method of soybean enzymatic hydrolyzate is as follows: take trypsin and flavor enzyme respectively, and formulate a compound enzyme according to the weight...

Embodiment 3

[0067] The micro-ecological type sea cucumber special feed of this embodiment is made by mixing the culture medium fermented by probiotics and the sea cucumber wet material, and the parts by weight of each component in the fermented culture medium are respectively: 5 parts of triple probiotics (wherein Bacillus subtilis: Enterococcus faecium: Streptococcus thermophilus (1.5:1.5:1), 64 parts of bran, 8 parts of starch, 18 parts of soybean enzymatic hydrolyzate, mix the above materials as the base material, add 3 parts of chitosan oligosaccharide Combine with 2 parts of glucose, mix with water at 5% by weight, incubate in an incubator at 37°C for 12 hours after mixing, and then mix with sea cucumber wet material at a weight ratio of 5% of sea cucumber wet material.

[0068] The production method of the soybean enzymatic hydrolyzate is as follows: take trypsin and flavor enzyme respectively, and make a compound enzyme according to the weight ratio of 4:1, and the compound enzyme a...

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Abstract

The invention relates to a special micro-ecological feed for stichopus japonicus and a preparation method thereof, belonging to the technical field of aquaculture feed processing. The feed is preparedby mixing a culture medium fermented by probiotics with a wet sea cucumber material, wherein the fermented culture medium comprises the following components in parts by weight: 4-6 parts of triple probiotics, 2-4 parts of chitosan oligosaccharide, 56-64 parts of bran, 2-3 parts of glucose, 7-9 parts of starch and 18-22 parts of soybean zymolyte; and the components are mixed and then cultured in an incubator at 37-42 DEG C for 10-14 hours, and then the mixture is mixed with the wet sea cucumber material according to the weight ratio of 4-6% of the wet sea cucumber material. The preparation method comprises the following steps: 1) preparing the soybean zymolyte; 2) preparing the wet sea cucumber material; 3) preparing the triple probiotics; 4) weighing; 5) activating; 6) mixing for the second time; and 7) extruding and granulating. According to the special micro-ecological feed for stichopus japonicus and the preparation method thereof, the intestinal tracts of the stichopus japonicus are regulated and controlled by adopting a probiotic combination which can be planted in the intestinal tracts of the stichopus japonicus and can stabilize the microbial phase in the intestinal tracts,so that the effect of fixing the structures of intestinal flora is exerted, the utilization rate of nutrient substances is increased, and the growth of the stichopus japonicus is promoted.

Description

technical field [0001] The present invention relates to a kind of micro-ecological sea cucumber special feed and its production method. Specifically, it is a technical method for regulating the intestinal tract of sea cucumber by adopting a combination of probiotics that can be planted in the intestinal tract of sea cucumber and stabilize the microbial phase in the intestinal tract The invention belongs to the technical field of aquaculture feed processing. Background technique [0002] In recent years, the rise of sea cucumber farming has shifted people's attention to the intestinal health of sea cucumbers. As beneficial microorganisms that live directly in the intestinal tract of animals, probiotics can regulate the growth rate of animals, improve the digestion and absorption of nutrients and enhance the body's immunity through various channels, including promoting the development of intestinal mucosal villi and producing antibacterial biological activities Substances, se...

Claims

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Application Information

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IPC IPC(8): A23K50/80A23K10/12A23K10/18A23K10/37A23K20/163A23K20/147A23K10/30A23K10/22A23K20/174A23K10/26A23K20/00A23K20/24A23K20/26A23K20/22A23K20/20A23K40/10A23K40/00
CPCA23K50/80A23K10/12A23K10/18A23K10/37A23K20/163A23K20/147A23K10/30A23K10/22A23K20/174A23K10/26A23K20/00A23K20/24A23K20/26A23K20/22A23K20/30A23K40/10A23K40/00Y02A40/818Y02P60/87
Inventor 宋志东李培玉黄炳山李宝山郝甜甜孙永智
Owner 山东省海洋资源与环境研究院