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Application of isosaxifragin in preparation of medicine for protecting rat against pine cone injury

A technology of iso-saxifrage and pineal gland, which is applied in the field of medicine, can solve the problems of undiscovered iso-saxifrage in ultrastructure, tissue morphology, molecular level, etc.

Active Publication Date: 2020-10-09
SHANXI PROVINCE CHINESE MEDICINE RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the prior art has not reported that saxifrage has a direct protective effect on the damage of the pineal gland of rats, nor has it been found that saxifrage has an effect on the ultrastructure, tissue morphology, and genes of the pineal gland of rats. Regulation and Direct Correlation of Apoptotic Mechanisms at the Molecular Level

Method used

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  • Application of isosaxifragin in preparation of medicine for protecting rat against pine cone injury
  • Application of isosaxifragin in preparation of medicine for protecting rat against pine cone injury
  • Application of isosaxifragin in preparation of medicine for protecting rat against pine cone injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1: Verification of the protective effect of IS on ultrastructural treatment of PCPA-induced pineal injury rat model

[0020] 1. Materials

[0021] 1. Drug: Isoxaxifrage monomer (IS): Shanghai Fusion Pharmaceutical Technology Co., Ltd., batch number: 150809, prepared into suspensions with different concentrations with 2% Tween-80 solution.

[0022] 2. Animals: rats, SD, SPF grade, male, weighing 160-180g, purchased from Speiford (Beijing) Biotechnology Co., Ltd., license number: SCXK (Beijing) 2016-0002; certificate number: No. 1103241911019500, raised in the central laboratory of Shanxi Academy of Traditional Chinese Medicine, temperature: 22-24°C, humidity: 40-50%.

[0023] 3. Reagents: p-chlorophenylalanine (PCPA): SIGMA Company of the United States, batch number: 10026000696; polysorbate-80 (Tween-80): chemical glass station of Hubei Pharmaceutical Company, batch number: 080308; chloral hydrate: Beijing Chaoyang District Chemical Pharmaceutical Factory, bat...

Embodiment 2

[0040] Example 2: Observation of the influence of IS on histopathological changes in rats with pineal gland injury:

[0041] A. Materials

[0042] Medicine: same as Example 1; Animal: same as Example 1; Reagent: same as Example 1.

[0043] Main instruments: universal microscope fluorescence imaging system: BX51+DPT2+IPE, Japan Olympus Company; automatic tissue dehydrator: EXCELSIOR ES, American Thermo SCIENTIFIC Company; rotary paraffin microtome: SHANDONFINESSE 325, American Thermo SCIENTIFIC Company; automatic sealing Machine: ClearVue, American Thermo Company; automatic dyeing machine: Gemini AS, American Thermo Company.

[0044] B. Method

[0045] Grouping and administration: same as in Example 1; pineal gland injury model: same as in Example 1.

[0046] C. Index detection: pineal gland histopathological observation: pineal gland was taken, fixed with 10% formaldehyde solution, dehydrated, paraffin embedded, sectioned, HE stained, observed under a microscope.

[0047] ...

Embodiment 3

[0048] Example 3: Effect of IS on the Regulation of Pineal Clock Genes in PCPA Injured Rats

[0049] 1. Pineal clock gene detection: real-time fluorescent quantitative (Real-Time PCR) was used to detect the expression of pineal clock genes Clock, Bmal1, Per1, Per2, Per3, Cry1, and Cry2 in rats with PCPA pineal injury.

[0050] (1) Extraction of total RNA: The extraction steps of total RNA were performed according to the instructions of the kit.

[0051] A. Take the pineal gland, fully grind the pineal gland in liquid nitrogen, add 1ml of Buffer RLT, pipette repeatedly, the sample is fully lysed, and stand at room temperature for 5 minutes.

[0052] B. Add 200 μl of chloroform to each 1ml of Buffer RLT and mix thoroughly, place at room temperature for 2 minutes, centrifuge at 12000 rpm for 5 minutes, and take the supernatant. Add the same volume of 70% ethanol, mix well, centrifuge at 12000rpm for 20s, and discard the waste liquid.

[0053] C. Add 350μl of Buffer RW1, centrif...

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Abstract

The invention belongs to the technical field of medicines, and provides a new application of isosaxifragin, and an application of isosaxifragin in preparation of a protective drug for rat pine cone injury. The rat pine cone body injury is rat pine cone body injury caused by p-chlorophenylalanine PCPA. The IS alleviates the superstructure and pathological structure of pine cone cells of a PCPA-induced rat pine cone injury model, has a regulation effect on pine cone clock genes, has a repairing effect on pine cone injury apoptotic cells, has the capability of improving the learning and memory ofthe model, promotes MT synthesis and secretion, and plays a role in protecting pine cones.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to the application of isosaxifragen in the preparation of a drug for protecting the pineal gland of rats. Background technique [0002] The pineal gland is located in the triangular gap between the two cerebral hemispheres and the cerebellum, the top is connected with the meninges, and the bottom is connected with the top of the third ventricle. It is an important part of the circadian rhythm biological clock and synthesizes and secretes melatonin Melatonin (MT), which promotes sleep, is called a "physiological hypnotic". After the pineal gland is damaged, the synthesis and secretion of MT decreases, which causes the body's circadian rhythm disorder. As a circadian biological clock, the pineal gland regulates the circadian rhythm, which in turn affects the body's learning and memory. The pineal gland of aged rats was surgically removed, and the Morris water maze test ...

Claims

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Application Information

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IPC IPC(8): A61K31/37A61P25/00A61P25/28
CPCA61K31/37A61P25/00A61P25/28
Inventor 仝立国仲启明王若瑜王丹妮郭晋良牛艳艳贾力莉杨钤白崇智胡文卓宋美卿吉海杰张夏林冯玛莉
Owner SHANXI PROVINCE CHINESE MEDICINE RES INST
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