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Application of tannic acid to preparation of anti-coronavirus medicine

A coronavirus and tannic acid technology, applied in antiviral agents, applications, pharmaceutical formulations, etc., can solve problems such as unproven safety and effectiveness, no drugs with exact curative effects, and discounts on antiviral effects. The development of drugs with a good foundation, significant inhibitory effect, and definite curative effect

Active Publication Date: 2020-10-13
GUANGZHOU SHENGPU INVESTMENT MANAGEMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the above-mentioned drugs are prone to adverse reactions such as diarrhea, anemia, dizziness, headache, and weakness
In addition, clinical studies have found that Remdesivir (CAS: 1809249-37-3) has certain activity against coronavirus pathogens such as atypical pneumonia (SARS) and Middle East respiratory syndrome (MERS), but Remdesivir has not been approved for marketing in any country, and its safety and effectiveness have not been confirmed
It can be seen that the current anti-coronavirus drugs have certain safety hazards, and with the mutation of the virus and the emergence of new strains, the anti-viral effect of these chemical drugs is greatly reduced. For example, the new coronavirus 2019-nCoV ( SARS-CoV-2), until now no effective drug has been found

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 medicine is to the mensuration of used cell line toxicity

[0039] 1. Experimental materials

[0040] Drugs: experimental group, tannic acid; control group, remdesivir (purchased from Shanghai Taosu Biochemical Technology Co., Ltd.);

[0041] Cell line: African green monkey kidney cells (Vero-E6 cells);

[0042] MEM medium; 96-well culture plate.

[0043] 2. Experimental steps

[0044] (1) The microculture method was used for cell culture.

[0045] (2) Drug dilution: In 12 centrifuge tubes, tannic acid and remdesivir were used in cell culture medium [ingredient requirements: MEM medium (Gibco Invitrogen), 1% double antibody (Gibco Invitrogen), 2% fetal bovine Serum (FBS; GibcoInvitrogen)] double-diluted to a total of 12 concentrations of tannic acid and remdesivir solutions.

[0046] (3) Set up 3 parallel replicate wells for each concentration, and set up cell control wells at the same time (do not add drugs, only add culture solution).

[0047] (4) A...

Embodiment 2

[0051] Embodiment 2 Tannic acid anti-SARS-CoV-2 virus efficacy test

[0052] 1. Experimental materials:

[0053] (1) Drugs: experimental group, tannic acid; control group, remdesivir (purchased from Shanghai Taosu Biochemical Technology Co., Ltd.).

[0054] (2) Other experimental materials

[0055] The virus is SARS-CoV-2; the cell line is African green monkey kidney cells (Vero-E6 cells); MEM medium; 96-well culture plate.

[0056] 2. Experimental steps

[0057] (1) Culture Vero-E6 cells into monolayer cells with 96-well culture plate in advance (the number of cells is: 2×10 4 cells / well);

[0058] (2) Select the maximum non-toxic dose of the drug to be tested (TD 0 ) medicinal liquid, respectively diluted 12 concentrations with cell maintenance solution, and the virus infection titer is 100TCID 50 ;

[0059] (3) 3 wells for each drug concentration, after the virus was adsorbed for 1 hour, the virus liquid was discarded, washed once with PBS, and 0.2ml of maintenance l...

Embodiment 3

[0069] Embodiment 3 A kind of spray of anticoronaviridae virus

[0070] An anti-coronaviridae virus spray contains the following components and their weight percentages: 10% of tannic acid, 5% of catechin, 3% of cosolvent and the balance of deionized water.

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Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to an application of tannic acid to preparation of an anti-coronavirus medicine. Live virus experiments show that the tannic acid has a remarkable inhibition effect on coronavirus such as SARS-CoV-2, the effect is remarkably higher than that of Remdesivir, the curative effect is exact, and the tannic acid has a wide application prospect in the field of preparation of anti-coronavirus medicines. The tannic acid exists in various plants, is a natural active compound existing in the nature, is also applied as a food additive, and is high in safety and good in medicine development foundation.

Description

technical field [0001] The invention belongs to the technical field of biomedicine. More specifically, it relates to the application of tannic acid in the preparation of anti-coronavirus drugs. Background technique [0002] In terms of systematic classification, coronaviruses belong to the order Nidovirales, the family Coronaviridae, and the genus Coronavirus. Among them, the viruses of the coronavirus genus are RNA viruses with an envelope and a linear single-stranded positive strand genome, and are a large class of viruses that exist widely in nature. The diameter of coronavirus is about 80-120nm, the 5′ end of the genome has a methylated cap structure, and the 3′ end has a poly(A) tail. The total length of the genome is about 27-32kb. . Coronaviruses only infect vertebrates, including humans. Currently, coronaviruses known to infect humans and cause severe outbreaks include SARS-CoV, SARS-CoV-2, MERS-CoV, HCoV-229E, HCoV-NL63, and HCoV-OC43 , HCoV-HKU1, which can caus...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7024A61P31/14A23L33/10A23K50/20A23K50/40A23K50/30A23K50/50A23K50/70A23K20/163
CPCA61K31/7024A61P31/14A23L33/10A23K50/20A23K50/40A23K50/30A23K50/50A23K50/70A23K20/163A23V2002/00A23V2200/30A23K20/24A23K50/75A61K33/00A61K33/06A61K33/26A61K33/30A61K2300/00A61K9/0053A61K9/12A61K31/05A61K31/353A61K31/7034A61K33/24
Inventor 付军
Owner GUANGZHOU SHENGPU INVESTMENT MANAGEMENT
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