Herba epimedii medicinal material extract and application thereof in prevention or treatment of coronavirus
A technology of coronavirus and herbal medicine, which is applied in the application field of Chinese medicinal materials, to achieve the effects of improving body immunity, inhibiting replication, and preventing amplification
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Embodiment 1
[0048] Analysis of the interaction between the components of Epimedium herb extract and the S-RBD protein of SARS-CoV-2 virus
[0049] (1) SPR chip surface pretreatment
[0050] The SARS-CoV-2 virus S-RBD protein was coupled to the surface of the esterified chip by covalent bonding. Select the covalent coupling of amino groups, specifically including the following steps:
[0051] The first step, chip activation: use the cross-linking agent EDC / NHS to esterify the chip surface under the condition of pH 4.5-5;
[0052] The second step, ligand coupling: coupling the SARS-CoV-2 virus S-RBD protein with a protein purity of more than 90% to the surface of the chip;
[0053] The third step, blocking: use 1M pH 8.5 ethanolamine hydrochloride to block the redundant active carboxyl groups on the chip.
[0054] The design of the control surface: the SPR chip is divided into four Flow Cell channels 1, 2, 3, and 4. Generally, channels 1 and 2 are used in pairs (channel 1 is used as a re...
Embodiment 2
[0065] Analysis of the effect of components of epimedium medicinal material extract and human receptor protein ACE2
[0066] Except for the surface pretreatment step of the SPR chip, other steps are the same as those in Embodiment 1, and will not be repeated here.
[0067] The SPR chip surface pretreatment of the present embodiment comprises:
[0068] The human receptor protein ACE2 was coupled to the surface of the esterified chip by covalent bonding. Select the covalent coupling of amino groups, specifically including the following steps:
[0069] The first step, chip activation: use the cross-linking agent EDC / NHS to esterify the chip surface under the condition of pH 4.5-5;
[0070] The second step, ligand coupling: the human receptor protein ACE2 with a protein purity of more than 90% is coupled to the surface of the chip;
[0071] The third step, blocking: use 1M pH 8.5 ethanolamine hydrochloride to block the redundant active carboxyl groups on the chip.
[0072] The...
Embodiment 3
[0077] Analysis of the Competitive Binding of Epimedium Herb Extract and Human Receptor ACE2 Protein to S-RBD Protein of SARS-CoV-2 Virus
[0078] (1) The surface pretreatment of the SPR chip is the same as that in Embodiment 1, and will not be repeated here.
[0079] (2) Sample injection
[0080] An extract of epimedium medicinal material, comprising the following components by weight ratio: 0.5 parts of epimedin A, 0.5 parts of epimedin B, 0.5 parts of epimedin C, 2.5 parts of icariin, 2 parts of Icariside-II, 2 parts of Icariside-I, and 2 parts of Icaritin. And the total concentration of the above-mentioned components in the extract is detected by conventional methods in the art.
[0081] Dilute the Epimedium medicinal material extract of this embodiment with running buffer (50mM phosphate, 100mM sodium chloride, 0.01% Tween-20, pH 7.4) to the total concentration of the above components respectively 50μM, 25μM, 12.5μM, 6.25μM, 3.125μM, 1.5625μM, 0.78125μM, mixed the dilu...
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