Universal single primer, kit and method for identifying tridacna crocea, tridacna derasa and hippopus hippopus
A technology of Tridacna safranus and a single primer, which is applied in biochemical equipment and methods, microbe measurement/inspection, DNA/RNA fragments, etc., can solve the problems of differentiation and small difference in gene sequence, and achieve low cost and simple method , the result is intuitive
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[0021] Example 1:
[0022] See figure 1 , Use sterilized tweezers and small scissors to cut a small amount of tissue from the mantle of Saffron crustacean, non-scale crustacean, and clam oyster. Add 400 μl of lysate and 10 μl of proteinase K (10 mg / ml) to the centrifuge tube containing the mantle tissue, mix on a shaker, and digest in a water bath at 55° C. until the tissue is completely dissolved to obtain the first solution. Add an equal volume of saturated phenol (200μl), chloroform / isoamyl alcohol (24:1) (200μl) mixture for extraction three times, centrifuge at 10000rpm for 10-15min each time, and aspirate the supernatant with a pipette. Add 1 mL of pre-cooled absolute ethanol to the centrifuge tube containing the supernatant, and settle overnight. Finally, it was washed with 70% alcohol, dried at room temperature, and dissolved in 200 μL of ultrapure water. UV spectrophotometer was used to detect DNA concentration and purity, and 1% agarose gel electrophoresis was used to...
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