Method for evaluating growth condition of peas
A pea and axillary bud technology, applied in the field of assessing the growth of peas, can solve problems such as restricting plant growth and development and intensifying competition
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Embodiment 1
[0022] Example 1: Detection of apical dominance in peas
[0023] After the plants were decapped, the growth of axillary buds was observed and recorded. Scan, record and measure each axillary bud according to the following method.
[0024] (1) Fix the paper with the line drawn on the glass culture dish to make a line drawing board;
[0025] (2) Put the ruler on the scanner and scan it into an image to obtain the ruler picture;
[0026] (3) Cut pea axillary buds from the main stem, and place each section of axillary buds on the line drawing board in the order of the nodes;
[0027] (4) According to the order of the sections, each section of the axillary buds is gradually transferred to the scanner and scanned into an image to obtain a picture of the axillary buds;
[0028] (5) Utilize ImageJ image processing software to process image, specifically:
[0029] A. Run the ImageJ software, open the scale picture, use the straight line tool to select a distance of more than 3cm fr...
Embodiment 2
[0033] Example 2: Evaluation of the effect of boron treatment on pea growth.
[0034] Pea seedlings in the normal boron concentration (25μmol.L -1 ) and a boron-deficient environment for about 3 weeks, according to the above-mentioned method, the axillary buds of the differently treated plants were placed in order of nodes, and the growth conditions were recorded. From figure 2 It can be seen intuitively that the apical dominance of boron-deficient plants is removed, the terminal buds wither, the axillary buds develop, and the reproductive development is inhibited (no flower buds) (in figure 2 Among them, node 0 is the pea cotyledon insertion point, node 1 is the first node, and so on; pea axillary buds are placed in the order of nodes. The measurement and statistics of bud length do not include the flower buds developed after 7 / 8 nodes treated with boron).
[0035] The length of axillary buds at each node was analyzed, and it was found that the differences in the growth ...
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