Unlock instant, AI-driven research and patent intelligence for your innovation.

Continuous cropping-resistant biological bacterial fertilizer special for cucumbers

A technology of biological bacterial fertilizer and anti-heavy cropping, applied in the field of agricultural microorganisms, can solve the problems of no combination, lack, lack of technical means to supplement potassium elements, etc.

Pending Publication Date: 2020-10-23
秦皇岛禾苗生物技术有限公司
View PDF8 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the component formulations of cucumber anti-replanting bacterial agents disclosed in the above two documents, there is a lack of Trichoderma fungi that have antagonistic effects on cucumber wilt pathogens and cucumber root-knot nematodes, or lack of technical means for supplementing potassium elements, etc. The allocator did not make the allocation according to the main cause of the damage caused by repeated cropping of cucumbers

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Continuous cropping-resistant biological bacterial fertilizer special for cucumbers
  • Continuous cropping-resistant biological bacterial fertilizer special for cucumbers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: Preparation of a kind of cucumber special-purpose anti-cropping biological bacterial fertilizer of the present invention

[0034] 1) Prepare Bacillus subtilis J-5 bacterial powder. Pick up a loop of the Bacillus subtilis strain slant with the preservation number CGMCC No.11750 with an inoculation needle, and inoculate it into a 50ml beef extract liquid medium (1000ml of this medium has a formula of: 3g beef extract, 10g peptone, 5g NaCl and 1000ml distilled water, in the 250ml shaking flask of pH7.0~7.2.), repeat above-mentioned inoculation process 8 times, promptly inoculate Bacillus subtilis bacterial strain to 8 same 250ml of 50ml beef extract liquid culture mediums. Shake flask; cultured on a shaker at 35°C and 200r / min for 36 hours to obtain a spore concentration of 8×10 8 ~10×10 8 The spore suspension of cfu / ml; The 400ml culture fluid of above-mentioned cultivation is transferred to the 15L PB liquid culture medium that is housed (the formula of t...

Embodiment 2

[0037] Embodiment 2: Preparation of a kind of cucumber special-purpose anti-cropping biological bacterial fertilizer of the present invention

[0038] 1) Prepare Bacillus subtilis J-5 bacterial powder. Pick up a loop of the Bacillus subtilis strain slant with the preservation number CGMCC No.11750 with an inoculation needle, and inoculate it into a 50ml beef extract liquid medium (1000ml of this medium has a formula of: 3g beef extract, 10g peptone, 5g NaCl and 1000ml distilled water, in the 250ml shaking flask of pH7.0~7.2.), repeat above-mentioned inoculation process 8 times, promptly inoculate Bacillus subtilis bacterial strain to 8 same 250ml of 50ml beef extract liquid culture mediums. Shake flask; cultured on a shaker at 35°C and 200r / min for 36 hours to obtain a spore concentration of 8×10 8 ~10×10 8 The spore suspension of cfu / ml; The 400ml culture fluid of above-mentioned cultivation is transferred to the 15L PB liquid culture medium that is housed (the formula of t...

Embodiment 3

[0041] Embodiment 3: Preparation of a kind of cucumber special-purpose anti-cropping biological bacterial fertilizer of the present invention

[0042] 1) Prepare Bacillus subtilis J-5 bacterial powder. Pick up a loop of the Bacillus subtilis strain slant with the preservation number CGMCC No.11750 with an inoculation needle, and inoculate it into a 50ml beef extract liquid medium (1000ml of this medium has a formula of: 3g beef extract, 10g peptone, 5g NaCl and 1000ml distilled water, in the 250ml shaking flask of pH7.0~7.2.), repeat above-mentioned inoculation process 8 times, promptly inoculate Bacillus subtilis bacterial strain to 8 same 250ml of 50ml beef extract liquid culture mediums. Shake flask; cultured on a shaker at 35°C and 200r / min for 36 hours to obtain a spore concentration of 8×10 8 ~10×10 8 The spore suspension of cfu / ml; The 400ml culture fluid of above-mentioned cultivation is transferred to the 15L PB liquid culture medium that is housed (the formula of t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a continuous cropping-resistant biological bacterial fertilizer special for cucumbers. The continuous cropping-resistant biological bacterial fertilizer special for cucumbers isprepared from 10 parts 16 parts by weight of bacillus subtilis J-5 bacterial powder; 6 to 12 parts by weight of paenibacillus mucilaginosus powder; 18-24 parts of trichoderma harzianum bacterial powder, 18-26 parts of trichoderma asperellum bacterial powder, 20-28 parts of paecilomyces lilacinus bacterial powder, 0.1-1 part of chitosan, 20-30 parts of potassium humate, 20-30 parts of monopotassium phosphate, 40-50 parts of diatomite and the like. The bacterial fertilizer gives full play to the characteristics of multifunctional bacteria such as bacillus subtilis J-5 and trichoderma harzianum;the multiple effects of beneficial substances such as potassium humate are brought into full play, the mutual synergistic effect among beneficial bacteria is fully utilized, the continuous cropping problem caused by cucumber fusarium wilt, root-knot nematode disease and soil potassium deficiency is solved in a targeted mode, and a good prevention and treatment effect on cucumber continuous cropping obstacles is achieved.

Description

technical field [0001] The invention relates to the technical field of agricultural microbes, in particular to a special anti-replantation biological bacterial fertilizer for cucumbers. Background technique [0002] my country's cucumber cultivation area is about 19 million mu, and the facility cultivation area accounts for about 42%. With the increase of cultivation years, the harm caused by continuous cropping (heavy cropping) of cucumber has become a key issue affecting the production of cucumber facility cultivation in my country. After 4 years, the yield of cucumbers decreased by 50%; if the crops were cultivated continuously for more than 8 years, the harvest of cucumbers was close to failure. The causes of the above-mentioned repeated cropping hazards include: soil-borne diseases and insect pests, autotoxicity, lack of organic matter and trace elements, etc. Among them, the damage caused by soil-borne diseases and insect pests (mainly cucumber wilt and root-knot nema...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C05G3/00C05G3/60C12N1/20C12N1/14C12R1/125C12R1/885C12R1/79C12R1/01
CPCC05B7/00C05G3/00C05G3/60C12N1/20C12N1/14
Inventor 宋春光崔继荣张宝海韩丽丽赵彦民
Owner 秦皇岛禾苗生物技术有限公司