Synthetic method for GHK copper peptides

A synthesis method and copper peptide technology are applied in the field of GHK copper peptide synthesis in industrial mass production, which can solve the problems of unstable parameter requirements, uneconomical and environmental protection of GHK copper peptide products, and achieve high yield, low cost and controllable parameters. Effect

Active Publication Date: 2020-10-23
CHINA JILIANG UNIV +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the disadvantages of uneconomical and environmental protection caused by the use of resin in the existing method, and the parameter requirements of GHK co

Method used

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  • Synthetic method for GHK copper peptides
  • Synthetic method for GHK copper peptides
  • Synthetic method for GHK copper peptides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Dissolve 0.54g of copper acetate in 20ml of distilled water, add 1.02g of GHK tripeptide under stirring conditions, stir and react at room temperature (30°C) for 3 hours, filter and concentrate, then add 1ml of diethylamine, stir for 30min, add 90ml of ethanol precipitated solids, filtered, washed the filter cake with distilled water and then dissolved in 10ml of distilled water, added 0.13ml of glacial acetic acid, stirred and reacted for 1 hour and then concentrated and freeze-dried to obtain 0.905g of GHK copper peptide complex with a purity of 98%. The rate is 72%, the acetic acid content is 16.1%, and the copper content is 10.6%.

Embodiment 2

[0041] Dissolve 0.54g of copper acetate in 20ml of distilled water, add 1.02g of GHK tripeptide under stirring conditions, stir and react at room temperature (30°C) for 3 hours, filter and concentrate, then add 0.216g of sodium hydroxide, and stir for 30 minutes. Add 70ml of ethanol to precipitate the solid, filter, wash the filter cake with distilled water and then dissolve it in 10ml of distilled water, add 0.13ml of glacial acetic acid, stir and react for 1h, then concentrate and freeze-dry to obtain 0.857g of GHK copper peptide complex with a purity of 98%. The yield is 67%, the acetic acid content is 16.9%, and the copper content is 9.88%.

Embodiment 3

[0043] Dissolve 0.25g of copper dimethoxide in 15ml of distilled water, add 0.68g of GHK tripeptide under stirring conditions, stir and react at room temperature (30°C) for 72h, filter and concentrate, then add 0.06ml of glacial acetic acid, and stir for 1h After concentration and freeze-drying, 0.414 g of GHK copper peptide complex with a purity of 99% was obtained, with a yield of 52%, an acetic acid content of 17.7%, and a copper content of 6.8%.

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Abstract

The invention provides a synthetic method for obtaining high-purity GHK copper peptides. According to the method, the post-treatment meanses of complexing GHK tripeptides with a copper complexing agent, performing filtering, preforming rotary drying, performing freeze drying and the like are taken, and high-purity GHK copper peptides can be obtained. The obtained copper complexing agent is selected from one or a mixture of two of copper acetate, copper hydroxide and copper methoxide. The synthetic method mainly has the advantages that compared with a conventional synthetic method, the synthetic method is simpler to operate and lower in cost, application of resins is avoided, the GHK copper peptide products being high in purity, high in yield and qualified and stable in sample parameters can be obtained, and the synthetic method can be applied to mass production.

Description

technical field [0001] The invention relates to a simple and convenient method for obtaining high-purity GHK copper peptide, especially a method for synthesizing GHK copper peptide that can control parameters such as acetic acid content in the copper peptide to meet requirements and be stable, and can be used in large-scale industrial production. Background technique [0002] Copper peptide (GHK-Cu) is a compound formed by the complexation of copper ions and GHK tripeptide, which consists of glycine-histidine-lysine-copper (glycyl-L-histidyl-L-lysine-copper) , originally isolated from human plasma in 1973, can improve blood circulation in medicine, promote the synthesis of nerves and neurotrophic factors, and has anti-oxidation, anti-inflammation and cancer treatment effects. In 1999, researchers believed that copper peptides and their copper restorations can be used as activators of tissue remodeling, as well as a signal peptide, and are now mainly used in skin care product...

Claims

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Application Information

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IPC IPC(8): C07K5/083C07K1/107
CPCC07K5/0806
Inventor 周益峰陈祉文杨晓章郭一平陈杨毅毛志峰
Owner CHINA JILIANG UNIV
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