Preparation method of electrochemical luminescence immunosensor for detecting CYFRA 21-1 by using copper-doped Tb-based MOF
A technology of luminescence immunity and copper doping, which is applied in the fields of chemiluminescence/bioluminescence, electrochemical variables of materials, scientific instruments, etc., can solve the problems of unstable luminescent signal and difficult fixation, and achieve high selectivity and high sensitivity detection, Quick response and simple operation
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Embodiment 1
[0031] 1. Preparation method of electrochemiluminescence immunosensor for detection of CYFRA 21-1 by copper-doped Tb-based MOF
[0032] (1) Preparation of Cu-doped Tb-based MOF
[0033] 2 mL of 20 mM TbCl 3 aqueous solution and 2 mL of 20 mM Cu(NO 3 ) 2 The solution was added to 5 mL of N,N-dimethylformamide, and 2 mL of phthalic acid with a concentration of 40 mM was added under stirring conditions. After magnetic stirring for 20 minutes, the above solution was transferred to the autoclave in 160°C for 3 hours. After cooling to room temperature, centrifuge, wash, and dry to obtain copper-doped Tb-based MOF;
[0034]In order to enhance the conductivity and biocompatibility of copper-doped Tb-based MOF, 4.3 mg of Pluronic F127 and 0.35 mg of cetylpyridinium chloride monohydrate were dissolved in 2.5 mL of distilled water, and the Add 30 μL of HAuCl at a concentration of 10 mM sequentially under rapid stirring 4 solution, 120 μL of 10 mM Na 2 PdCl 4 solution and 120 μL o...
Embodiment 2
[0048] 1. Preparation method of electrochemiluminescence immunosensor for detection of CYFRA 21-1 by copper-doped Tb-based MOF
[0049] (1) Preparation of Tb-based MOF
[0050] 3 mL of 20 mM TbCl 3 aqueous solution and 3 mL of 20 mM Cu(NO 3 ) 2 The solution was added to 5 mL of N,N-dimethylformamide, and 3 mL of phthalic acid with a concentration of 40 mM was added under stirring conditions. After magnetic stirring for 20 minutes, the above solution was transferred to the reaction kettle , 160°C for 3 hours. After cooling to room temperature, centrifuge, wash, and dry to obtain copper-doped Tb-based MOF;
[0051] In order to enhance the conductivity and biocompatibility of copper-doped Tb-based MOF, 4.3 mg of Pluronic F127 and 0.35 mg of cetylpyridinium chloride monohydrate were dissolved in 2.5 mL of distilled water, and the Add 30 μL of HAuCl at a concentration of 10 mM sequentially under rapid stirring 4 solution, 120 μL of 10 mM Na 2 PdCl 4 solution and 120 μL of 1...
Embodiment 3
[0065] 1. Preparation method of electrochemiluminescence immunosensor for detection of CYFRA 21-1 by copper-doped Tb-based MOF
[0066] (1) Preparation of Tb-based MOF
[0067] Add 6 mL of 20 mM TbCl 3 aqueous solution and 6 mL of 20 mM Cu(NO 3 ) 2 The solution was added to 5 mL of N,N-dimethylformamide, and 6 mL of phthalic acid with a concentration of 40 mM was added under stirring conditions, and after magnetic stirring for 20 minutes, the above solution was transferred to the autoclave in 160°C for 3 hours. After cooling to room temperature, centrifuge, wash, and dry to obtain copper-doped Tb-based MOF;
[0068] In order to enhance the conductivity and biocompatibility of copper-doped Tb-based MOF, 4.3 mg of Pluronic F127 and 0.35 mg of cetylpyridinium chloride monohydrate were dissolved in 2.5 mL of distilled water, and the Add 30 μL of HAuCl at a concentration of 10 mM sequentially under rapid stirring 4 solution, 120 μL of 10 mM Na 2 PdCl 4 solution and 120 μL o...
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