Preparation method of electrochemical luminescence immunosensor for detecting CYFRA 21-1 by using copper-doped Tb-based MOF

A technology of luminescence immunity and copper doping, which is applied in the fields of chemiluminescence/bioluminescence, electrochemical variables of materials, scientific instruments, etc., can solve the problems of unstable luminescent signal and difficult fixation, and achieve high selectivity and high sensitivity detection, Quick response and simple operation

Inactive Publication Date: 2020-10-27
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Traditional electrochemiluminescent materials such as luminol and ruthenium terpyridine have disadvantages such as difficult fixation and unstable luminescence signals.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Preparation method of electrochemiluminescence immunosensor for detection of CYFRA 21-1 by copper-doped Tb-based MOF

[0032] (1) Preparation of Cu-doped Tb-based MOF

[0033] 2 mL of 20 mM TbCl 3 aqueous solution and 2 mL of 20 mM Cu(NO 3 ) 2 The solution was added to 5 mL of N,N-dimethylformamide, and 2 mL of phthalic acid with a concentration of 40 mM was added under stirring conditions. After magnetic stirring for 20 minutes, the above solution was transferred to the autoclave in 160°C for 3 hours. After cooling to room temperature, centrifuge, wash, and dry to obtain copper-doped Tb-based MOF;

[0034]In order to enhance the conductivity and biocompatibility of copper-doped Tb-based MOF, 4.3 mg of Pluronic F127 and 0.35 mg of cetylpyridinium chloride monohydrate were dissolved in 2.5 mL of distilled water, and the Add 30 μL of HAuCl at a concentration of 10 mM sequentially under rapid stirring 4 solution, 120 μL of 10 mM Na 2 PdCl 4 solution and 120 μL o...

Embodiment 2

[0048] 1. Preparation method of electrochemiluminescence immunosensor for detection of CYFRA 21-1 by copper-doped Tb-based MOF

[0049] (1) Preparation of Tb-based MOF

[0050] 3 mL of 20 mM TbCl 3 aqueous solution and 3 mL of 20 mM Cu(NO 3 ) 2 The solution was added to 5 mL of N,N-dimethylformamide, and 3 mL of phthalic acid with a concentration of 40 mM was added under stirring conditions. After magnetic stirring for 20 minutes, the above solution was transferred to the reaction kettle , 160°C for 3 hours. After cooling to room temperature, centrifuge, wash, and dry to obtain copper-doped Tb-based MOF;

[0051] In order to enhance the conductivity and biocompatibility of copper-doped Tb-based MOF, 4.3 mg of Pluronic F127 and 0.35 mg of cetylpyridinium chloride monohydrate were dissolved in 2.5 mL of distilled water, and the Add 30 μL of HAuCl at a concentration of 10 mM sequentially under rapid stirring 4 solution, 120 μL of 10 mM Na 2 PdCl 4 solution and 120 μL of 1...

Embodiment 3

[0065] 1. Preparation method of electrochemiluminescence immunosensor for detection of CYFRA 21-1 by copper-doped Tb-based MOF

[0066] (1) Preparation of Tb-based MOF

[0067] Add 6 mL of 20 mM TbCl 3 aqueous solution and 6 mL of 20 mM Cu(NO 3 ) 2 The solution was added to 5 mL of N,N-dimethylformamide, and 6 mL of phthalic acid with a concentration of 40 mM was added under stirring conditions, and after magnetic stirring for 20 minutes, the above solution was transferred to the autoclave in 160°C for 3 hours. After cooling to room temperature, centrifuge, wash, and dry to obtain copper-doped Tb-based MOF;

[0068] In order to enhance the conductivity and biocompatibility of copper-doped Tb-based MOF, 4.3 mg of Pluronic F127 and 0.35 mg of cetylpyridinium chloride monohydrate were dissolved in 2.5 mL of distilled water, and the Add 30 μL of HAuCl at a concentration of 10 mM sequentially under rapid stirring 4 solution, 120 μL of 10 mM Na 2 PdCl 4 solution and 120 μL o...

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Abstract

The invention relates to a preparation method of an electrochemical luminescence immunosensor for detecting CYFRA 21-1 by using a copper-doped Tb-based MOF. Specifically, The copper-doped Tb-based MOFis adopted as a sensing interface, and the copper-doped Tb-based MOF has a large specific surface area and a stable electrochemical luminescence signal. The AuPdPt nanoflower is compounded with the copper-doped Tb-based MOF, so that the conductivity of the copper-doped Tb-based MOF can be improved, and the noble metal nanoflower has catalytic activity and can further amplify an electrochemical luminescence signal. The linear range of CYFRA 21-1 detection is 0.1 pg. mL<-1>-100 ng. mL<-1>, and the detection limit is 32 fg.mL<-1>.

Description

technical field [0001] The invention relates to a preparation method of an electrochemiluminescence immunosensor for detecting CYFRA 21-1 by a copper-doped Tb-based MOF. Specifically, a composite of copper-doped Tb-based MOF and AuPdPt nanoflowers is used as a luminescent material and a sensing interface to prepare an electrochemiluminescence immunosensor for detecting CYFRA 21-1, which belongs to the field of electrochemiluminescence sensors. Background technique [0002] The increasing incidence of lung cancer makes us pay attention to the early and accurate diagnosis and treatment of lung cancer in order to prolong the life of patients and improve their quality of life. Therefore, it is urgent to find sensitive and specific biochemical indicators to assist diagnosis. Cytokeratin 19 fragment 21-1 (CYFRA21-1) is a soluble fragment of cytokeratin 19. When tumor cells die or dissolve, a large amount of CYFRA 21-1 will be released into the blood circulation of tumor patients,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48G01N21/76
CPCG01N27/305G01N27/308G01N27/3277G01N27/3278G01N27/48G01N21/76G01N21/763
Inventor 鞠熀先周丽敏魏琴任祥王超范大伟吴丹马洪敏
Owner UNIV OF JINAN
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