Stem cell cryopreservation method and cell cryopreservation solution used by stem cell cryopreservation method
A cryopreservation method and stem cell technology, applied in the field of stem cell preservation and cultivation, can solve the problems of affecting the effect of clinical treatment, introducing pollution and allergens, and being unsuitable for application, so as to maintain cell osmotic pressure, prolong cryopreservation time, and prevent mechanical damage effect
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[0042] Example: cryopreservation of stem cells, the cell cryopreservation solution used includes the following components: liquid I and liquid II, wherein liquid I includes human albumin, bFGF and EGF, and the concentration of bFGF is: 1% w / v, the concentration of EGF is: 1% w / v, and the volume is adjusted to 100ml with human serum albumin.
[0043] II solution includes components: Knockout Serum replacement, cytochrome C, betaine, antifreeze protein, glucose, low molecular weight dextran, mannan, sodium chloride, sodium lactate, potassium chloride, calcium chloride and Dimethyl sulfoxide, wherein: the concentration of cytochrome C is: 1.2% w / v, the concentration of betaine is: 0.3% w / v, the concentration of antifreeze protein is: 0.3% w / v, the concentration of glucose is: : 3.5% w / v, low molecular weight dextran concentration: 1.5% w / v, mannan concentration: 1% w / v, sodium chloride concentration: 0.1% w / v, sodium lactate Concentration of: 0.1% w / v, concentration of sodium l...
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