Tumor vessel normalization detection system and detection method
A tumor blood vessel and detection system technology, applied in the tumor blood vessel normalization detection system and detection field, can solve the problems of difficult to guarantee accuracy, high cost, complicated operation, etc., and achieve accurate judgment and prediction, easy extraction, and obvious technical advantages. Effect
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Embodiment 1
[0069] [Example 1] Tumor blood vessel normalization detection system
[0070] The system and method for detecting normalization of tumor blood vessels according to the embodiments of the present invention. refer to figure 1 As shown, the system consists of a laser light source module, an imaging module, an image processor, a blood flow detection and analysis module, a blood vessel morphology analysis module, an artificial intelligence analysis module and a display module.
[0071] The laser light emitted by the He-Ne laser is firstly attenuated by a variable attenuator, then the beam diameter and divergence angle of the laser are changed by a beam expander, and finally the laser is evenly irradiated to the surface of tumor tissue by a reflector. The light reflected by the tumor tissue is magnified by a stereo microscope and collected by a CCD camera. The CCD camera transmits the collected speckle images to the blood flow detection and analysis module for blood flow image rec...
Embodiment 2
[0106] [Example 2] In a lung cancer xenograft model, the vascular normalization detection device guides Melafolone to enhance the efficacy of anti-PD-1 therapy
[0107] (1) Grouping and handling of animals
[0108] Mouse lung cancer cells LLC and CMT167 (5×10 6 ) were subcutaneously inoculated into C57BL / 6 mice. The tumor-bearing mice were randomly divided into blank control group, PD-1 monoclonal antibody group, Melafolone (Mel, which can induce normalization of tumor blood vessels) group, PD-1 monoclonal antibody and Mel combination group.
[0109] (2) Experimental detection
[0110] 1. Record the survival rate: observe the survival of the mice every day, and make a survival curve.
[0111] 2. Determination of tumor weight: The animals were sacrificed 24 hours after the last administration, the tumor was completely stripped off, the color and texture of the tumor tissue samples were observed, and the tumor weight was weighed with an electronic balance to calculate the tum...
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