Preparation method of microparticles for preventing novel coronavirus and application

Abstract

The invention provides a preparation method of microparticles for preventing novel coronavirus and an application, and relates to the field of vaccines. Specifically, the preparation method comprisesthe following steps: 1) constructing recombinant plasmids for overexpression of a spike (S) protein; 2) packaging the recombinant plasmids into lentiviral particles; 3) infecting fibroblasts with thelentiviral particles to obtain tool cells for expressing of S protein; 4) performing X-ray irradiation on the obtained tool cells and a culture solution, and collecting supernatant after radiotherapyto obtain a mixture of required microparticles and apoptotic tool cell debris; and 5) performing centrifuging, concentrating and purifying on the obtained mixture to obtain a vaccine. According to theinvention, the microparticles released by radiotherapy induction tool cells are used as a carrier, so that the antiviral immunity of an organism can be directly activated under the condition of overexpression of the novel coronavirus S protein, and a neutralizing antibody for resisting the novel coronavirus is generated. According to the invention, an organism can be induced to generate a high-level neutralizing antibody immune response, and besides, good biological safety is achieved.

Description

【Technical field】 [0001] The invention relates to the technical field of vaccines, in particular to a preparation method and application of microparticles for preventing novel coronaviruses. 【Background technique】 [0002] The 2019 novel coronavirus pneumonia epidemic is defined by the World Health Organization as an international public health emergency. The disease is a type of acute respiratory infectious disease caused by a novel coronavirus (SARS-CoV-2) infection. Respiratory droplet transmission, aerosol transmission and contact transmission. Common symptoms include fever, cough, shortness of breath and difficulty breathing. Severe acute respiratory syndrome, renal failure and even death may occur in severe infection. [0003] The diameter of coronavirus is usually between 70-120nm. It is an enveloped positive-sense single-stranded RNA virus, and its single-stranded non-segmented RNA genome length is between 26-32kb. Coronaviruses belong to the Coronaviridae or Corona...

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to solve the need for tedious expression and purification steps in the preparation process of recombinant protein vaccines, and to provide a preparation method for preventing new coronavirus microparticles and its application as a vaccine

Images