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Preparation method of microparticles for preventing novel coronavirus and application

A technology of coronavirus and lentiviral particles, which is applied in the field of vaccines, can solve the problems of complicated expression and purification steps, and achieve the effects of simple preparation process, good biosafety and biocompatibility, and reduced production costs

Inactive Publication Date: 2020-11-03
WUHAN SHENGRUN BIOTECHNOLOGY CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] The purpose of the present invention is to solve the need for tedious expression and purification steps in the preparation process of recombinant protein vaccines, and to provide a preparation method for preventing new coronavirus microparticles and its application as a vaccine

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  • Preparation method of microparticles for preventing novel coronavirus and application
  • Preparation method of microparticles for preventing novel coronavirus and application
  • Preparation method of microparticles for preventing novel coronavirus and application

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Embodiment Construction

[0040] The following examples serve to illustrate the invention, but do not limit the scope of the invention. On the premise of not departing from the spirit and essence of the present invention, any modifications or replacements made to the methods, steps or conditions of the present invention belong to the scope of the present invention.

[0041] In the present invention, after X-rays are used to irradiate genetically engineered fibroblasts, the cells will secrete microparticles rich in novel coronavirus S protein. The obtained microparticles can induce the body to produce anti-new coronavirus antibodies. In the present invention, the storage conditions for preventing novel coronavirus microparticles are 4°C and within 7 days. The preventive novel coronavirus microparticle structure described in the present invention is as follows: figure 1 As shown, the particle size is as figure 2 shown.

[0042] The following are embodiments of the invention:

[0043] 1. Construct r...

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Abstract

The invention provides a preparation method of microparticles for preventing novel coronavirus and an application, and relates to the field of vaccines. Specifically, the preparation method comprisesthe following steps: 1) constructing recombinant plasmids for overexpression of a spike (S) protein; 2) packaging the recombinant plasmids into lentiviral particles; 3) infecting fibroblasts with thelentiviral particles to obtain tool cells for expressing of S protein; 4) performing X-ray irradiation on the obtained tool cells and a culture solution, and collecting supernatant after radiotherapyto obtain a mixture of required microparticles and apoptotic tool cell debris; and 5) performing centrifuging, concentrating and purifying on the obtained mixture to obtain a vaccine. According to theinvention, the microparticles released by radiotherapy induction tool cells are used as a carrier, so that the antiviral immunity of an organism can be directly activated under the condition of overexpression of the novel coronavirus S protein, and a neutralizing antibody for resisting the novel coronavirus is generated. According to the invention, an organism can be induced to generate a high-level neutralizing antibody immune response, and besides, good biological safety is achieved.

Description

【Technical field】 [0001] The invention relates to the technical field of vaccines, in particular to a preparation method and application of microparticles for preventing novel coronaviruses. 【Background technique】 [0002] The 2019 novel coronavirus pneumonia epidemic is defined by the World Health Organization as an international public health emergency. The disease is a type of acute respiratory infectious disease caused by a novel coronavirus (SARS-CoV-2) infection. Respiratory droplet transmission, aerosol transmission and contact transmission. Common symptoms include fever, cough, shortness of breath and difficulty breathing. Severe acute respiratory syndrome, renal failure and even death may occur in severe infection. [0003] The diameter of coronavirus is usually between 70-120nm. It is an enveloped positive-sense single-stranded RNA virus, and its single-stranded non-segmented RNA genome length is between 26-32kb. Coronaviruses belong to the Coronaviridae or Corona...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/165C12N15/867A61K39/215A61P31/14A61P11/00
CPCC07K14/005C12N15/86A61K39/12A61P31/14A61P11/00C12N2770/20034C12N2770/20022C12N2770/20023C12N2740/15043
Inventor 黄浩金红林卢利森万超郑俊武郑成武卫路洪磊金秀妍刘群
Owner WUHAN SHENGRUN BIOTECHNOLOGY CO LTD
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