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Silicon-based bioactive glass composite hydrogel having self-healing characteristic, preparation method thereof and application of silicon-based bioactive glass composite hydrogel to myocardium repair

A bioactive glass and composite hydrogel technology, which is applied in the fields of material technology, biomedicine, myocardial repair and medical devices, can solve the clinical use of injectable hydrogels that are unfavorable for clinical practice and unfavorable myocardial repair, and the effect has not been verified. and other problems, to achieve the effect of improving the self-healing efficiency and the ability to inhibit the apoptosis of cardiomyocytes

Active Publication Date: 2020-11-10
SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the patent only verified that the above-mentioned composite hydrogel can be used to fill the myocardial defect to play a supporting role and promote the vascularization of the damaged myocardium, and did not verify its effect in stem cell therapy in the damaged myocardium
[0007] Chinese invention patent CN106267364A discloses a conductive porous scaffold of alginic acid / PEDOT, which can promote the differentiation of brown adipose stem cells into cardiomyocytes through electrical stimulation, but this method requires additional electrical stimulation, and the operation is complicated, which is not conducive to clinical practice
[0008] Chinese patent CN109503863A discloses a chitosan-based temperature-sensitive hydrogel loaded with umbilical cord mesenchymal stem cells (UCMSCs) for cardiac injury repair of myocardial infarction, but this hydrogel has temperature-sensitive properties and needs to be mixed in an ice bath. Gelation, complex operation, and the gelation time is relatively short (about 3 minutes), which is not conducive to the clinical use of injectable hydrogel for myocardial repair

Method used

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  • Silicon-based bioactive glass composite hydrogel having self-healing characteristic, preparation method thereof and application of silicon-based bioactive glass composite hydrogel to myocardium repair
  • Silicon-based bioactive glass composite hydrogel having self-healing characteristic, preparation method thereof and application of silicon-based bioactive glass composite hydrogel to myocardium repair
  • Silicon-based bioactive glass composite hydrogel having self-healing characteristic, preparation method thereof and application of silicon-based bioactive glass composite hydrogel to myocardium repair

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] 1. Aldehyde modification of γ-PGA (polyglutamic acid): Dissolve 0.30 g of γ-PGA powder in 60 mL of deionized water and stir to dissolve completely. Then, 0.42 g of aminopropanediol and 0.31 g of N-hydroxysuccinimide were added. After it is fully dissolved, adjust the pH to 4.0 with 0.1M NaOH solution or 0.1M HCl solution. Then, 0.89 g of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride was added, and the reaction was carried out at room temperature for 24 hours. Pour into a dialysis bag (with a molecular weight cut-off of 30,000 Da) and dialyze for 3 days to obtain an aminopropanediol-modified γ-PGA solution. Then, under dark conditions, add 0.02 g of sodium periodate, react for 5 minutes, and stop by adding excess ethylene glycol. Finally, pour the obtained aldehyde-modified γ-PGA solution into a dialysis bag (with a molecular weight cut-off of 30000Da) for 3 days and change the water twice a day. After freeze-drying, the aldehyde-modified γ-PGA is obtained...

Embodiment 2

[0051] Mix 5% aldehyde-modified γ-PGA solution and 5% 2-hydroxypropionic acid-modified chitosan solution in equal volume ratio, and add 1.00% bioactive glass to make bioglass Composite hydrogel. The gel forming time of the composite hydrogel is 3 minutes. Two composite hydrogels were prepared in parallel, one of which was dyed green with dye. Then, cut the two parts of hydrogel in half, exchange them, and re-splice them together. After 5 minutes, the hydrogels can be spliced ​​together stably, and the spliced ​​interface will not be broken by pulling hard. This is because: the structure of Schiff base is formed by condensation reaction of aldehyde group and amino group. Through dehydration and hydrolysis process, the chemical bond formed by Schiff base is in dynamic and reversible equilibrium, which also provides the self-healing performance of hydrogel basis. After adding the silicon-based bioactive glass, since the silicon-based bioactive glass can continue to degrade and...

Embodiment 3

[0053] Mix 5% aldehyde-modified γ-PGA solution and 5% 2-hydroxypropionic acid-modified chitosan solution in equal volume ratio, and add 0.500% bioactive glass to make bioglass Composite hydrogel.

[0054] The stem cells used are human bone marrow mesenchymal stem cells (hBMSCs, Cyagen Biosciences), and the culture medium used is the Cyagen adult bone marrow mesenchymal stem cell complete medium provided by the manufacturer. The culture condition is a constant temperature incubator at 37℃, and the atmosphere is 5% CO 2 , The frequency of medium replacement is 3 days / time. When the cell density reaches 80.0%, the medium is removed, washed with PBS, then digested, centrifuged, and subcultured to continue culture. Take the 3rd to 8th generation MSCs for experimental research.

[0055] The left descending anterior branch ligated mice were randomly divided into 3 groups, namely: myocardial infarction group (AMI group), MSC treatment group (MSC group), and hydrogel wrapped MSC treatment...

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Abstract

The invention provides silicon-based bioactive glass composite hydrogel having self-healing characteristic, a preparation method thereof and an application of the silicon-based bioactive glass composite hydrogel to myocardium repair. The silicon-based bioactive glass composite hydrogel has a cross-linked gel network and a composite gel structure, wherein the cross-linked gel network is formed by forming an imine bond through a Schiff base reaction between an aldehyde group-containing high polymer material and an amino-containing high polymer material through aldehyde groups and amino, and a composite gel structure formed by uniformly dispersing silicon-based bioactive glass in the gel network; and the silicon-based bioactive glass composite hydrogel contains the following components in percentage by mass: of 1-10% of the aldehyde group-containing high polymer material, 1-10% of the amino-containing high polymer material and 0.1-1.0% of the silicon-based bioactive glass.

Description

Technical field [0001] The invention relates to the fields of biomedicine, material technology, myocardial repair and medical equipment, in particular to a silicon-based bioactive glass composite hydrogel for treating myocardial injury, and a preparation method and application thereof. Background technique [0002] Cardiovascular disease is currently one of the most important diseases of human death worldwide. The World Health Organization (WHO) reported that although medical technology and medical quality have been significantly improved in recent years, 17.5 million people died of cardiovascular disease globally in 2012, accounting for 31% of the total deaths, far exceeding other single diseases The death toll even exceeds the total death toll from various cancers. According to the "China Cardiovascular Disease Report 2014" issued by the National Center for Cardiovascular Diseases, the total hospitalization expenses for patients with acute myocardial infarction in 2013 reached...

Claims

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Application Information

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IPC IPC(8): A61L27/46A61L27/38A61L27/50A61L27/52A61L27/54C08J3/075C08J3/24C08L77/04C08L5/08C08G69/50
CPCA61L27/46A61L27/3834A61L27/3826A61L27/50A61L27/52A61L27/54C08J3/075C08J3/246C08G69/50A61L2400/06A61L2430/30A61L2300/30A61L2300/412C08J2377/04C08J2405/08C08J2477/04C08J2305/08C08L5/08C08L77/04C08L5/04C08L5/02
Inventor 常江高龙周艳玲
Owner SHANGHAI INST OF CERAMIC CHEM & TECH CHINESE ACAD OF SCI
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