Ssr Molecular Marker and Its Application

Abstract

The present invention provides a cypress SSR molecular marker and its application. The numbers of the molecular marker are: JSSSR4, JSSSR5, JSSSR6, JSSSR15, JSSSR17, JSSSR20, JSSSR30, JSSSR31, JSSSR33, JSSSR35, JSSSR54, JSSSR57, JSSSR58, JSSSR61, JSSSR66 , JSSSR74 and JSSSR80, the sequence of the primer pair of SSR molecular markers corresponding to each number is shown in SEQ ID NO.1‑34. The 17 pairs of stinky cypress SSR molecular markers of the present invention have the advantages of rich polymorphism, stable amplification, good repeatability, and convenient statistics, etc., and can effectively perform genetic diversity analysis and population clustering analysis on stinky cypress germplasm resources, and can It showed good polymorphism and interspecific generality among the closely related species of Skunk cypress.

Description

technical field [0001] The present invention relates to the technical field of molecular biology DNA markers, in particular, to a SSR molecular marker developed based on GBS simplified genome sequencing and its application. Background technique [0002] Juniperus sabina L., also known as fork juniper, sand cypress, Xinjiang juniper, etc., is an evergreen coniferous shrub of the genus Juniperaceae, distributed intermittently in Spain, Kazakhstan, China, Mongolia and Russia. Naturally distributed in China from the Tianshan Mountains to the Altai Mountains in Xinjiang, Helan Mountains in Ningxia, Mu Us Sandy Land and Hunshandake Sandy Land in Inner Mongolia, northeastern Qinghai, Qilian Mountains in Gansu, Shenmu and Yulin in Shaanxi and other places. It has developed root system, strong germination and tillering ability, strong adaptability to drought, cold and wind erosion, and branches are resistant to sand burial. It is an excellent tree species for windbreak, sand fixation...

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Problems solved by technology

[0003] Although stinky cypress has important ecological value, medicinal value, economic value and feed value, there are very few studies on its genetic resources, only RAPD (Random Amplified Polymorphic, random amplified DNA) markers and chloroplast gene fragments, etc. A small amount of research, limited by a single labeling method, Geng et al. (2016) developed 8 pairs of SSR (Simple Sequence Repeat, simple repeat sequence) primers through double suppression PCR (suppression-PCR) technology, but the number is too small to cover genomic loci. Satisfies the needs of genetic diversity analysis of stinky cypress; Qu Na et al. (2011) used 17 RAPD marker primers to make a preliminary analysis of the genetic diversity of 4 variant types of stinky cypress, but the RAPD marker technology is a dominant marker, and the obtained information The amount is relatively small, and the stability of labeling is poor, and it is easily interfered by equipment, reaction conditions and operating methods, resulting in poor polymorphism and indistinguishable band migration.

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