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Zwitterionic polymer for implantable sensor and preparation method of zwitterionic polymer

A technology of zwitterions and polymers, which is applied in the direction of instruments, scientific instruments, analytical materials, etc., can solve the problems of increasing the production cycle and cost of sensors, reducing the concentration of analytes, etc., to improve efficacy and performance, improve detection sensitivity, and strong resistance The effect of interference ability

Active Publication Date: 2020-11-20
杭州柏医健康科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, while the external coating film improves the biocompatibility of the sensor, it also reduces the concentration of the analyte; the additional coating process also increases the production cycle and cost of the sensor

Method used

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  • Zwitterionic polymer for implantable sensor and preparation method of zwitterionic polymer
  • Zwitterionic polymer for implantable sensor and preparation method of zwitterionic polymer
  • Zwitterionic polymer for implantable sensor and preparation method of zwitterionic polymer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] Example 1 SBMA and NH 2 -(CH 2 ) 6 (m-bim)Os(bpy) 2 2PF 6 Preparation of Zwitterionic Polymers by Polymerization

[0055] The thiobetaine methyl acrylate (SBMA) used in this example was purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., the osmium complex NH 2 -(CH 2 ) 6 (m-bim)Os(bpy) 2 2PF 6 For the synthesis of this research group, refer to the synthetic route in AnIntrinsic Self-Charging Biosupercapacitor Combined of a High-Potential Bioanode and a Low-Potential Biocathode, other reagents are known products, obtained by purchasing commercially available products.

[0056] (1) Copolymerization of acrylic acid (AA) and thiobetaine methyl acrylate (SBMA) monomer solution: Weigh 2.21g of AA and 0.04g of SBMA monomer in turn in a polymerization bottle filled with a certain amount of 0.3M NaCl solution, shake to make it Completely dissolved, nitrogen at room temperature. After adding 0.0018g of potassium persulfate and sodium bisulfite (mass ratio...

Embodiment 2

[0058] Example 2 Electrochemical characterization (CV) of p(SBMA-AA-Os) modified electrode

[0059] Using the p(SBMA-AA-Os) prepared in Example 1, prepare a pbs solution of 10 mg / ml p(SBMA-AA-Os), pipette 2 microliters of the solution to the surface of the glassy carbon electrode and dry it as a membrane-modified electrode , the electrochemical experiment was carried out on Chenhua CHI600 electrochemical workstation, 10mL electrolytic cell, three-electrode system: the membrane-modified glassy carbon electrode was used as the working electrode, Ag / AgCl was used as the reference electrode, the platinum wire electrode was used as the counter electrode, and the bottom solution was tested It is a phosphate buffer solution of pH 7.4, and the result of cyclic voltammetry is as follows figure 2 Shown, and compared with commonly used ferrocene-modified electrodes.

[0060] figure 2 The voltammogram of the zwitterionic polymer clearly shows specific peaks for the oxidation and subse...

Embodiment 3

[0061] Example 3 Stability test of p(SBMA-AA-Os) modified electrode

[0062] Using the p(SBMA-AA-Os) modified electrode prepared in Example 2, in the in vitro experiment, the stability test of the electrode was performed using a newborn bovine whole serum solution stored at 37° C. to simulate the in vivo environment. Impedance (Z')-time (T) scanning was performed on the p(SBMA-AA-Os) modified electrode in neonatal bovine serum at 37°C, and the results were as follows image 3 shown, reflecting its performance in an in vitro environment. Depend on image 3It can be seen that the impedance of the p(SBMA-AA-Os) modified electrode only increased slightly in the first few minutes, and then quickly reached equilibrium and remained stable at 230Ω; after the glassy carbon bare electrode GCE was immersed in serum, its impedance increased rapidly, from 330Ω It quickly rose to 550Ω, and the overall impedance was nearly doubled, and it began to stabilize after about 1 hour. It can be s...

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Abstract

The invention provides a sensor for an implantable sensor, in particular relates to a zwitterionic polymer of implantable glucose sensors. The invention also provides a preparation method of the polymer. The zwitterionic polymer containing a redox ligand is obtained; the problem of biocompatibility in vivo can be solved in the inner layer (inert electrode surface) of the sensor; electrons betweenenzyme electrodes can be quickly transferred; the measurement result is reliable and stable, the problem that a coating process needs to be added to the outer surface of the sensor of the implantableglucose sensor is solved, the enzyme electrode for the implantable sensor can be prepared through a one-step method, the process is simple, the enzyme electrode can be directly used in vivo or in a complex solution environment, and the detection sensitivity and accuracy of the implantable sensor are obviously improved.

Description

technical field [0001] The invention relates to the technical field of polymer preparation, in particular to a zwitterionic polymer used for implanted sensors and a preparation method thereof. Background technique [0002] With the improvement of living standards and the rapid development of microelectronics technology, implantable sensors for real-time monitoring of analyte concentration have attracted more and more attention. Such sensors can convert biochemical reaction signals based on analyte concentration into electrical or optical signals, and are widely used in the detection of analytes in clinical, environmental, agricultural, and biotechnological applications. Analytes that can be measured by implantable sensors in the clinical analysis of body fluids include glucose, lactate, cholesterol, bilirubin, and amino acids, among others. [0003] Implantable sensors that monitor analytes via electrical signals have attracted great attention because electron transfer is i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F220/06C08F220/38C08F8/32G01N27/327
CPCC08F220/06C08F8/32G01N27/3271C08F220/387
Inventor 王晓东陈玮宫宇山
Owner 杭州柏医健康科技有限公司
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