Dynamic brewing method of low-alcohol fruit wine

A mellow fruit wine and dynamic technology, applied in the field of food and beverage, can solve the problems of sugar-acid balance breakdown, damage to sensory experience, consumption, etc., to achieve the effect of ensuring health and safety, good sensory experience, and high nutritional value

Active Publication Date: 2020-11-20
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the fermentation of fruit wine, the sugar content is consumed in large quantities. One is to maintain the growth of yeast, and the other is to produce alcohol, which will lead to sugar and acid in the original juice The balance is sharply broken, which brings an extremely unbalanced over-sour and astringent taste to the fruit wine, destroying the sensory experience and reducing the purchasing power of consumers
Some manufacturers will add extra sugar in the late production of fruit wine. Although it can improve the taste, it will destroy the balance and unique flavor of the wine to a certain extent. Such wine is not worth tasting and buying.

Method used

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  • Dynamic brewing method of low-alcohol fruit wine
  • Dynamic brewing method of low-alcohol fruit wine
  • Dynamic brewing method of low-alcohol fruit wine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Alcohol-producing ability test of strain

[0026] TTC plate: 0.5g / L TTC, 5g / L glucose, 15g / L agar.

[0027] YPD plate: 10g / L yeast extract powder, 20g / L yeast peptone, 20g / L glucose, 20g / L agar.

[0028] The applicant used a strain of Pichia pastoris JT-1-3 obtained by the preliminary screening (this strain has been disclosed in the patent document of CN 108676736 A), as well as two other strains of common Saccharomyces cerevisiae RV002 and NP-7-5 in the laboratory. experimenting.

[0029] Spread JT-1-3, RV002 and NP-7-5 on YPD plates and incubate them at 28°C for 60 hours, then cover the TTC plates incubated at 50°C on the YPD plates and incubate at 28°C in the dark for 3 hours. Take pictures and record the results. The colorless TTC can be reduced to red TTF during the yeast production process. The darker the color, the stronger the yeast production capacity. The result is as follows figure 2 As shown in the figure, it can be seen that JT-1-3 shows weaker alcohol...

Embodiment 2

[0030] Example 2 Fermentation ability test of strain

[0031] VHG medium: 200g / L glucose, 8g / L yeast peptone, 10g / L yeast extract powder.

[0032] In this experiment, JT-1-3, RV002 and NP-7-5 were inoculated in a VHG medium slightly higher than the sugar concentration in the juice, and their fermentation ability was tested to determine their potential in fruit wine fermentation. JT-1-3, RV002 and NP-7-5 were inoculated into VHG medium at an inoculum of 3% (v / v), cultured at 28°C, 120r / min, and weighed every 12h Stop the experiment until its quality no longer drops. The results show that the fermentation speed of JT-1-3 is slightly weaker than that of ordinary Saccharomyces cerevisiae, but it can also enter the stable fermentation period at 72h, and the fermentation weight loss of JT-1-3 is slightly greater than that of Saccharomyces cerevisiae in the late fermentation period.

Embodiment 3

[0033] Example 3: Fermentation experiment of kiwi fruit wine

[0034] The following takes kiwi fruit wine as an example to further describe the dynamic brewing method of the present invention in detail.

[0035] 1) Wash the fresh kiwi fruit and peel it, squeeze the juice and add 0.2% pectinase to enzymatically treat it for 3 hours, then filter the juice and paste it for use;

[0036] 2) Activate the non-Saccharomyces cerevisiae-Pichia pastoris JT-1-3 to a bacterial content of 10 7 -10 8 CFU / mL, collect the yeast cells after centrifugation and wash them with sterile saline until there is no peculiar smell, then adjust the concentration of the bacterial solution to 10 with sterile water 10 -10 12 CFU / mL;

[0037] 3) Pass the adjusted concentration of yeast liquid into the fermentation tank (structure such as figure 1 (Shown), the fermentation tank is provided with several layers of fermentation plates staggered up and down, and the fermentation plate is composed of a plate ultrafiltratio...

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Abstract

The invention discloses a dynamic brewing method of low-alcohol fruit wine. The method comprises the following steps of firstly introducing yeast liquid into a fermentation tank, enabling yeast to beattached to a plate-type ultrafiltration membrane of a fermentation plate, then introducing sterilized fruit juice into the bottom of the fermentation tank, controlling the temperature in the fermentation tank to be 15-25 DEG C and the flow rate of the fruit juice to be 5-20 ml / min, enabling the fruit juice to be in full contact with the yeast on the plate-type ultrafiltration membrane, carrying out alcoholic fermentation, and collecting the fruit juice passing through the topmost layer of fermentation plate, namely obtaining the low-alcohol fruit wine. According to the method, dynamic brewingof the fruit wine is achieved; and compared with a conventional brewing method, the method has the advantages that the time needed by alcoholic fermentation can be remarkably shortened, the alcoholicstrength in the fruit wine is reduced, and meanwhile, a certain sugar degree is reserved, so that better sensory experience is achieved, and health and safety of consumers can be guaranteed.

Description

Technical field [0001] The invention belongs to the field of food and beverages, and specifically relates to a dynamic brewing method of low-alcohol fruit wine. Background technique [0002] Fruit wine is loved by consumers because of its unique taste, pleasant color and distinctive flavor characteristics. With the improvement of the quality of life, people pay more and more attention to the quality of fruit wine. At present, in the fruit wine manufacturing industry, fresh fruits with higher ripeness are generally selected for fermentation, and the sugar content in the initial fermentation liquid is higher, so that the wine has a higher alcohol content after the end of alcohol fermentation. Fruit wine is different from other wines. When the alcohol content is too high, it is likely to directly cause the fermentation to stagnate. This is mainly because the yeast selected in the fermentation will appear when the alcohol content exceeds 18% (v / v). Growth is stagnant, and even cell ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12G3/024C12G3/025C12R1/84
CPCC12G3/024C12G3/025Y02E50/10
Inventor 李二虎余策钟武潘思轶
Owner HUAZHONG AGRI UNIV
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