Macroporous hydrogel as well as preparation method and application thereof

A hydrogel and gelatin technology, applied in the field of macroporous hydrogel and its preparation, can solve the problems of difficult to prepare hydrogel with super large pore size, unfavorable material biocompatibility, lack of uniform pore structure, etc., and achieve good application Prospects, excellent biocompatibility and degradability, good biocompatibility

Active Publication Date: 2020-11-24
GUANGDONG PROV MEDICAL INSTR INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the common methods for preparing biological macroporous hydrogels mainly include salt leaching method, phase separation method, freeze-drying method, gas foaming method, etc., but these methods often lack uniform and interconnected pore structure and pore size, making it difficult to prepare Hydrogels with ultra-large pore size, or the need to introduce additional chemicals during the preparation process to increase toxicity, which is not conducive to the biocompatibility of the material

Method used

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  • Macroporous hydrogel as well as preparation method and application thereof
  • Macroporous hydrogel as well as preparation method and application thereof
  • Macroporous hydrogel as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: a kind of hydrogel and preparation method thereof:

[0050] (1) Get 0.3g of gelatin microspheres with a particle size of about 230 μm and place them in a polytetrafluoroethylene mold, and gently tap the mold to make the gelatin microspheres tightly packed and smooth on the surface;

[0051] (2) Inject the ethanol solution into the stacked gelatin microspheres, and inject about 1 mL of ethanol solution with a volume concentration of 80% per gram of gelatin microspheres;

[0052] (3) Move the mold to a 70°C oven for 8 minutes;

[0053] (4) Take out the mold and freeze-dry to obtain the mutually bonded gelatin microsphere template, and its SEM image is as follows figure 2 As shown, the adjacent microspheres are slightly bonded at the interface, forming a mutually bonded gelatin microsphere template;

[0054] (5) Put the gelatin microsphere template of step (4) into 1 mL of 2% bis In the bonded hyaluronic acid solution, under the condition of -0.08MPa, the...

Embodiment 2

[0056] Embodiment 2: a kind of hydrogel and preparation method thereof:

[0057] (1) Sieve out gelatin microspheres with a particle size of about 100 μm, take 0.2 g of gelatin microspheres with a particle size of about 100 μm and place them in a polytetrafluoroethylene mold, tap the mold gently to make the gelatin microspheres tightly packed and the surface smooth ;

[0058] (2) Inject the ethanol solution into the stacked gelatin microspheres, and inject about 1 mL of ethanol solution with a volume concentration of 80% per gram of gelatin microspheres;

[0059] (3) Move the mold to an oven at 80°C for 5 minutes;

[0060] (4) Take out the mold and freeze-dry to obtain interconnected gelatin microsphere templates;

[0061] (5) Mix 1% double-bond hyaluronic acid solution and 10% double-bond gelatin solution at a volume ratio of 10:1, add 0.1% phenyl-2,4,6-trimethylbenzoylphosphinic acid Lithium, to obtain a mixed solution; the gelatin microsphere template of step (4) is put i...

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Abstract

The invention provides macroporous hydrogel and a preparation method and application thereof. The preparation method comprises the steps that: water-soluble gelatin microspheres are adopted as a pore-foaming agent, gelatin microsphere interfaces are bonded with one another in a heating mode, a gelatin microsphere template is removed after crosslinking and gelling, and finally the macroporous hydrogel with controllable pore diameter and mutual communication is formed. Adhesion, migration and proliferation of cells and transportation of oxygen, nutrient substances and the like are facilitated, avascularization promoting gel stent is expected to be further developed, and application of the hydrogel in tissue engineering and regenerative medicine is expanded. Compared with macroporous hydrogel prepared by traditional methods, the macroporous hydrogel prepared by the method has the advantages that the gelation time is short, the gelation can be completed within a few seconds, the pore structure is controllable, uniform and mutually communicated, and the pore diameter range is 30-600 microns.

Description

technical field [0001] The invention belongs to the technical field of biomaterials, and in particular relates to a macroporous hydrogel and a preparation method and application thereof. Background technique [0002] Tissue engineering and regenerative medicine achieve tissue repair or regeneration by constructing tissue engineering scaffolds to simulate extracellular matrix and seed cells to form a complex. In order to better repair different damaged tissues, it is crucial to construct tissue engineering scaffolds with similar physical and chemical properties of extracellular matrix through biomimetic design. Hydrogel has been widely used in the field of tissue engineering and regenerative medicine because of its biomimetic structure highly similar to natural extracellular matrix, good mechanical properties, biocompatibility, and easy preparation. Three-dimensional culture and delivery system. [0003] However, the loaded cells are usually coated and confined in the submi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J9/26A61L27/24A61L27/22A61L27/20A61L27/52A61L27/56A61L27/58C08L5/08C08L89/00C08L5/10C08L5/04
CPCC08J9/26A61L27/24A61L27/222A61L27/227A61L27/20A61L27/52A61L27/56A61L27/58C08J2207/10C08J2201/0464C08J2205/022C08J2305/08C08J2489/00C08J2389/00C08J2305/10C08J2305/04A61L2400/08C08L5/08
Inventor 鲁道欢于珊耿志杰裴大婷纪禹行国翠平刘群峰
Owner GUANGDONG PROV MEDICAL INSTR INST
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