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Magnetic micro-module, preparation method thereof and cell culture method based on magnetic micro-module

A cell culture and micro-module technology, which is applied in the field of micron-scale operation and biological cell culture, can solve the problems of culture dish waste and cell damage, and achieve the effect of easy replacement

Active Publication Date: 2020-11-24
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the defects of the above-mentioned traditional cell culture method, the present invention provides a magnetic micromodule and its preparation method, and a cell culture method based on the magnetic micromodule, which can effectively solve the problem of a large number of culture dishes when culturing cells in a culture dish at the present stage. Waste issues, and cell damage when passaging cells by trypsinization, cell scraping, etc.

Method used

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  • Magnetic micro-module, preparation method thereof and cell culture method based on magnetic micro-module
  • Magnetic micro-module, preparation method thereof and cell culture method based on magnetic micro-module
  • Magnetic micro-module, preparation method thereof and cell culture method based on magnetic micro-module

Examples

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Effect test

Embodiment 1

[0052] A magnetic micromodule, comprising a magnetic parylene layer and a collagen layer attached to the magnetic parylene layer, wherein magnetic nanoparticles are mixed in the magnetic parylene layer.

[0053] Among them, the magnetic micromodule can be fixed in the culture dish under the adsorption of the magnetic adsorption plate, so as to achieve the purpose of not damaging the cells and the medium can be replaced.

Embodiment 2

[0055] Based on the above examples, this example produces micromodules suitable for single cell growth by smearing and photolithography. The chemical deposition method is used to precipitate a parylene solution mixed with magnetic nanoparticles with a diameter of 1-100nm; the metal Al layer 4 is attached to the parylene layer 3, and is produced by thermal evaporation and precipitation. Then use chemical etching method, that is, use strong acid or strong alkali solution to directly chemically etch the unprotected part of the metal Al layer 4, and obtain a specific micro-module shape on the bottom surface of the metal Al layer 4; use plasma air with flexible cutting and small deformation Cutting, cutting the sodium alginate layer 2 and the magnetic parylene layer 3 into the shape of the above-mentioned micromodule; the MPC hydrophobic layer 5 is used to prevent cells from growing on the wall of the non-micromodule part; the metal Al layer 4 and the MPC on the micromodule The hyd...

Embodiment 3

[0067] It can be seen from the above examples that the magnetic micromodules can be used for non-damage cell culture. Based on the above examples, this example provides a recyclable and low-damage cell culture method based on the magnetic micromodules, that is, in the magnetic adsorption plate. The micromodule is fixed under the action of adsorption, so that the medium can be replaced without damaging the cells; at the same time, the magnetic adsorption plate cooperates with micro-nano tweezers to realize the circular culture of cells, which specifically includes the following steps:

[0068] 1) if Figure 8 As shown, a plurality of micromodules are tiled irregularly in the petri dish 9 containing the cell culture medium, among which some micromodules 7 have cells attached to the surface, while some micromodules 8 are blank.

[0069] Wherein, the cell culture medium is a suspension containing cells to be cultured.

[0070] 2) if Figure 9 As shown, under suitable culture con...

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Abstract

The invention provides a magnetic micro-module, a preparation method thereof and a cell culture method based on the magnetic micro-module. The magnetic micro-module comprises a magnetic poly-p-xylylene layer and a collagen layer attached to the magnetic poly-p-xylylene layer, wherein to-be-cultured cells can be inoculated on the collagen layer, and the magnetic poly-p-xylylene layer can be matchedwith a magnetic adsorption plate placed below a culture dish, so that the micro-module can be fixed in the culture dish, replacement of a cell culture medium is facilitated, and the problem of wasteof a large number of culture dishes during cell culture in the culture dishes at the present stage is effectively solved; besides, during cell culture, the magnetic micro-module is flatly laid on theculture dish, the magnetic adsorption plate is fixed on the bottom of the culture dish, so that the micro-module cannot slip or move under magnetic adsorption, the defect of cell damage caused by cellpassage in modes of trypsin digestion, cell scrapers and the like in a traditional method is overcome, and the operation difficulty of a cell culture experiment is reduced.

Description

technical field [0001] The invention belongs to the technical field of micron-level operation and biological cell culture, and in particular relates to a magnetic micromodule, a preparation method thereof, and a cell culture method based on the magnetic micromodule. Background technique [0002] Today in the 21st century, while the material life has been greatly improved, people's attention to physical health is also increasing. The research in the field of biomedicine is dedicated to solving the existing medical problems, such as cancer, AIDS, various infectious diseases, etc., and providing a solid foundation for people's healthy life. Cell culture is the experimental basis of cell biology and an important research method in life research. [0003] Currently, researchers generally use cell culture dishes (bottles) to cultivate adherent cells. However, when the cell culture density reaches its peak, the growth and reproduction ability of the cells will decrease, which wil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M3/00C12M1/22C12N5/00
CPCC12N5/0068C12M23/40C12M23/10C12M23/50C12N2533/54
Inventor 刘晓明李磊李玉洋黄强新井健生
Owner BEIJING INSTITUTE OF TECHNOLOGYGY